DAPTA

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DAPTA

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

106362-34-9

D-Ala-peptide T-amide
Adaptavir

DAPTA 是一种合成肽，为CCR5抑制剂，同时具有抗 HIV 的活性。

生物活性

DAPTA is a synthetic peptide, functions as a viral entry inhibitor by targeting selectivelyCCR5, and shows potent anti-HIV activities.

IC₅₀& Target^[2]

gp120_BaL/sCD4-CCR5

55 pM (IC₅₀, in Cf2Th/synR5 cells)

gp120CM_CM235/sCD4-CCR5

0.32 nM (IC₅₀, in Cf2Th/synR5 cells)

体外研究
(In Vitro)

DAPTA (1 nM) inhibits HIV-1 replication in monocytes/macrophages (M/M) by >90%. DAPTA blocks HIV entry and prevents HIV-1 infection. DAPTA reduces CCR5 mAb binding in human primary macrophages. DAPTA potently blocks R5 gp120-mediated neuronal apoptosis. DAPTA is even more potent in preventing neuronal apoptosis than the CCR5 antagonist TAK-779^[1]. DAPTA potently inhibits specific CD4-dependent binding of gp120 Bal (IC₅₀= 0.06 nM) and CM235 (IC₅₀= 0.32 nM) to CCR5. DAPTA (1 nM) blocks formation of the gp120/sCD4 complex with CCR5. DAPTA inhibits the binding of gp120BaL/sCD4 to CCR5 (Cf2Th/synR5) cells with IC₅₀of 55 ± 0.08 pM^[2].

分子量

856.88

性状

Solid

Formula

C₃₅H₅₆N₁₀O₁₅

CAS 号

106362-34-9

Sequence

Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-NH2

Sequence Shortening

ASTTTNYT-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(58.35 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.1670 mL	5.8351 mL	11.6702 mL
5 mM	0.2334 mL	1.1670 mL	2.3340 mL
10 mM	0.1167 mL	0.5835 mL	1.1670 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 10 mg/mL (11.67 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。