Compstatin

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Compstatin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

206645-99-0

坎普他汀

Compstatin 是一个 13 残基环肽，是补体系统C3的有效抑制剂，具有物种特异性。Compstatin 结合到狒狒的 C3，并对狒狒血液中的蛋白水解裂解具有抗性（类似人类）。Compstatin 只抑制灵长类补体系统的激活。Compstatin 对补体经典途径和旁路途径的 IC₅₀值分别为 63 μM 和 12 μM。

生物活性

Compstatin, a 13-residue cyclic peptide, is a potent inhibitor of thecomplement systemC3with species specificity. Compstatin binds to baboon C3 and is resistant to proteolytic cleavage in baboon blood (similar to humans). Compstatin inhibits only the activation of primates’complement system. Compstatin exhibits IC₅₀values of 63 μM and 12 μM for classical and alterative complement pathway, respectively^[1]^[2]^[3]^[4].

体外研究
(In Vitro)

Compstatin exhibits an in vitro half-life in human blood of about2 hr^[2].
In solution, compstatin forms a β-turn at residues Gln-5–Gly-8 with the disulfide bridge Cys-2–Cys12, residues Ile-1–Val-4, and Thr-13, forming a hydrophobic cluster^[3].

体内研究
(In Vivo)

Compstatin (21 mg/kg) produces complete inhibition when given as a combination of bolus injection and infusion. Compstatin completely inhibits in vivo heparin/protamine-induced complement activation without adverse effects on heart rate or systemic arterial, central venous, and pulmonary arterial pressures^[1].
Compstatin is stable in baboon plasma for more than 24 h^[1].
Pig xenografts survival is significantly longer in the Compstatin perfused group than in the control group^[2].

Animal Model:	Juvenile baboons (P. Anubis) weighing 10.5-28.8 kg^[1].
Dosage:	50, 25 mg/kg 60 min after heparin and 2 min before protamine.
Administration:	A bolus injection.
Result:	Completely inhibited complement activation induced by heparin–protamine complexes.

分子量

1550.77

性状

Solid

Formula

C₆₆H₉₉N₂₃O₁₇S₂

CAS 号

206645-99-0

Sequence

Ile-Cys-Val-Val-Gln-Asp-Trp-Gly-His-His-Arg-Cys-Thr-NH2 (Disulfide bridge: Cys2-Cys12)

Sequence Shortening

ICVVQDWGHHRCT-NH2 (Disulfide bridge: Cys2-Cys12)

中文名称

坎普他汀

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-80°C	2 years
	-20°C	1 year
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 100 mg/mL(64.48 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.6448 mL	3.2242 mL	6.4484 mL
5 mM	0.1290 mL	0.6448 mL	1.2897 mL
10 mM	0.0645 mL	0.3224 mL	0.6448 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (64.48 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。