Ibuprofen ((±)-Ibuprofen) L-lysine 是一种口服有效的COX-1选择性抑制剂，IC50值为 13 μM。Ibuprofen L-lysine 抑制细胞增殖、血管生成，并诱导细胞凋亡 (apoptosis)。Ibuprofen L-lysine 是一种非甾体抗炎试剂和一氧化氮 (NO) 供体。Ibuprofen L-lysine 可用于疼痛、肿胀、炎症、感染、免疫学、癌症的研究。
| 生物活性 | Ibuprofen ((±)-Ibuprofen) L-lysine is a potent orally active, selectiveCOX-1inhibitor with anIC50value of 13 μM. Ibuprofen L-lysine inhibits cell proliferation, angiogenesis, and induces cellapoptosis. Ibuprofen L-lysine is a nonsteroidal anti-inflammatory agent and a nitric oxide (NO) donor. Ibuprofen L-lysine can be used in the research of pain, swelling, inflammation,infection, immunology, cancers[1][2][3][4][5][6][7][8]. |
| IC50& Target | IC50: 13 μM (COX-1), 370 μM (COX-2) |
体外研究
(In Vitro) | Ibuprofen (24 h) L-lysine inhibits COX-1 and COX-2 activity with IC50values of 13 μM and 370 μM[1].
Ibuprofen (500 μM, 48 h) L-lysine inhibits cell proliferation and angiogenesis, and induces apoptosis in AGS cells (Adenocarcinoma gastric cell line)[2].
Ibuprofen (500 μM, 48 h) L-lysine downregulates transcription of Akt, VEGF-A, PCNA, Bcl2, OCT3/4 and CD44 genes, but upregulates RNA levels of wild type P53 and Bax genes in AGS cell[2].
Ibuprofen (500 μM, 24 h) L-lysine restores microtubule reformation, microtubule-dependent intracellular cholesterol transport, and induces extension of microtubules to the cell periphery in both cystic fibrosis (CF) cell models and primary CF nasal epithelial cells[3].
Ibuprofen (500 μM, 24 h) L-lysine enhances UV-induced cell death in MCF-7 cells and MDA-MB-231 cells by a photosensitization process[4].
Cell Viability Assay[2] | Cell Line: | AGS cells | | Concentration: | 100-1000 μM | | Incubation Time: | 24 h, 48 h | | Result: | Inhibited AGS cell viability with IC50values of 630 μM (trypan blue staining, 24 h), 456 μM (neutral red assay, 24 h), 549 μM (trypan blue staining, 48 h) and 408 μM (neutral red assay, 48 h). |
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体内研究
(In Vivo) | Ibuprofen (300 mg/kg; p.o.; daily, for 14 days) L-lysine reduces overall tumor growth and enhances anti-tumor immune characteristics without adverse autoimmune reactions in a model of postpartum breast cancer[5].
Ibuprofen (60 mg/kg; i.h.; every second day for 15 days) L-lysine reduces the risk of neuropathy in a rat model of chronic Oxaliplatin-induced peripheral neuropathy[6].
Ibuprofen (20 mg/kg; p.o.; every 12 hours, 5 doses total) L-lysine decreases muscle growth (average muscle fiber cross-sectional area) without affecting regulation of supraspinatus tendon adaptions to exercise[7].
Ibuprofen (35 mg/kg; p.o.; twice daily) L-lysine attenuates the Inflammatory response topseudomonas aeruginosain a rat model of chronic pulmonary infection[8].
| Animal Model: | Syngeneic (D2A1) orthotopic Balb/c mouse model of PPBC (postpartum)[5] | | Dosage: | 300 mg/kg, daily for 14 days | | Administration: | Fed in animal feedings (added to pulverized standard chow and mixed dry, then mixed with water, made into chow pellets and dried thoroughly) | | Result: | Suppresed tumor growth, reduced presence of immature monocytes and increased numbers of T cells.
Enhanced Th1 associated cytokines as well as promoted tumor border accumulation of T cells. |
| Animal Model: | Oxaliplatin-induced peripheral neuropathy[6] | | Dosage: | 60 mg/kg, every second day for 15 days | | Administration: | Subcutaneous injection | | Result: | Lowered sensory nerve conduction velocity (SNCV). |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
