PACAP-38 (31-38), human, mouse, rat 是一种PAC1受体激活剂，增加 α-分泌酶活性。PACAP-38 (31-38), human, mouse, rat 升高胞浆 Ca2+，增加细胞外调节激酶 (ERK) 和表皮生长因子受体 (EGFR) 的增殖和磷酸化。PACAP-38 (31-38), human, mouse, rat 对交感神经 NPY 和儿茶酚胺的产生具有强效、持续的刺激作用。PACAP-38 (31-38), human, mouse, rat 可用于神经营养和神经保护研究。
| 生物活性 | PACAP-38 (31-38), human, mouse, rat is aPAC1receptoractivator and increases the α-secretase activity. PACAP-38 (31-38), human, mouse, rat elevates cytosolic Ca2+, increases proliferation and increases phosphorylation of extracellular regulates kinase (ERK) and the epidermal growth factor receptor (EGFR). PACAP-38 (31-38), human, mouse, rat demonstrates potent, efficacious, and sustained stimulatory effects on sympathetic neuronal NPY and catecholamine production. PACAP-38 (31-38), human, mouse, rat can be used for neurotrophic and neuroprotective research[1][2][3]. |
体外研究
(In Vitro) | PACAP-38 (31-38), human, mouse, rat (100 nM; 2 min; NCI-H838 cells) induces EGFR, HER2 and ERK tyrosine phosphorylation[1].
PACAP-38 (31-38), human, mouse, rat (100 nM; 30 min; NCI-H838 cells) induces EGFR tyrosine phosphorylation with generates ROS and increases ROS levels by 51%[1].
PACAP-38 (31-38), human, mouse, rat (10 nM; 48 h) stimulates the growth of NCI-H838 cells[1].
PACAP-38 (31-38), human, mouse, rat (300 nM; 4 h) stimulates generation of APPsα in neural cells[2].
PACAP-38 (31-38), human, mouse, rat (0.01-10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors by cAMP accumulation and by an increase in cytosolic free calcium and induces elevation of the intracellular Ca2+concentration in a dose-dependent manner with an EC50value of 0.81 nM[2].
PACAP-38 (31-38), human, mouse, rat (0.01 nM; 48 h) elicits potent and efficacious stimulation of NPY secretion from SCG neuronal cultures[3].
PACAP-38 (31-38), human, mouse, rat (100 nM; 14 d) produces sustained stimulated NPY and catecholamine secretionmpathetic neurons[3].
Cell Viability Assay[1] | Cell Line: | NCI-H838 cells | | Concentration: | 10 nM | | Incubation Time: | 48 hours | | Result: | Increased the number of NCI-H838 cells by 72%. |
Western Blot Analysis[1] | Cell Line: | 100 nM | | Concentration: | 100 nM | | Incubation Time: | 2 minutes | | Result: | Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively. |
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| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| Sequence | Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2 |
| Sequence Shortening | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Sealed storage, away from moisture | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 100 mg/mL(94.14 mM;Need ultrasonic) 配制储备液 | 1 mM | 0.9414 mL | 4.7069 mL | 9.4138 mL | | 5 mM | 0.1883 mL | 0.9414 mL | 1.8828 mL | | 10 mM | 0.0941 mL | 0.4707 mL | 0.9414 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
