ML334

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ML334

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1432500-66-7

LH601A

ML334 (LH601A) 是一种有效的，细胞可渗透的NRF2激活剂，通过抑制Keap1-NRF2蛋白质相互作用来发挥作用。ML334 以K_d值为 1μM 来结合 Keap1 Kelch 域。ML334 刺激NRF2表达和核易位并诱导抗氧化反应元件 (ARE) 活性。

生物活性

ML334 is a potent, cell permeable activator ofNRF2by inhibition ofKeap1-NRF2protein-protein interaction. ML334 binds to Keap1 Kelch domain with aK_dof 1 μM. ML334 stimulatesNRF2expression and nuclear translocation and induces antioxidant response elements (ARE) activity^[1]^[2].

IC₅₀& Target

Ki: 1 μM (Keap1)^[2]

体外研究
(In Vitro)

ML334 (LH601A; 50-100 μM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h^[1].
ML334 (LH601A; 50-100 μM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells^[1].
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells^[1].

RT-PCR^[1]

Cell Line:	HEK293 cells
Concentration:	50 μM or 100 μM
Incubation Time:	6 hours, 16 hours
Result:	Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h.

Western Blot Analysis^[1]

Cell Line:	HEK293 cells
Concentration:	50 μM or 100 μM
Incubation Time:	16 hours
Result:	Enhanced HO-1 and TRX1 protein expression at 16 h.

分子量

446.50

性状

Solid

Formula

C₂₆H₂₆N₂O₅

CAS 号

1432500-66-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据

In Vitro:

DMSO : 44.65 mg/mL(100.00 mM;Need ultrasonic)

Ethanol : 44.65 mg/mL(100.00 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.2396 mL	11.1982 mL	22.3964 mL
5 mM	0.4479 mL	2.2396 mL	4.4793 mL
10 mM	0.2240 mL	1.1198 mL	2.2396 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。