Isobavachalcone

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Isobavachalcone

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

20784-50-3

补骨脂乙素
Corylifolinin
Isobacachalcone

Isobavachalcone (Corylifolinin) 来源于补骨脂，是一种Akt信号通路的有效抑制剂，可诱导人类癌细胞凋亡 (以IC₅₀值为 7.92 μM 抑制 OVCAR-8 癌细胞生长)，具有抗癌和抗增殖活性。Isobavachalcone 还能诱导 OVCAR-8 细胞中活性氧的产生。

生物活性

Isobavachalcone (Corylifolinin) is derived fromPsoralea corylifolia Linn.and is a potent inhibitor ofAktsignaling pathway, which inducesapoptosisin humancancercells (Inhibits OVCAR-8 cell growth with anIC₅₀value of 7.92 μM). Isobavachalcone also inducesReactive Oxyen Species (ROS)generation in OVCAR-8 cells and has exhibitcanceranti-promotive and anti-proliferative activity^[1].

IC₅₀& Target

IC50: 7.92 μM (OVCAR-8 cell)^[1]

体外研究
(In Vitro)

Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC50 values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively^[1].
Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation^[1].
Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway^[1].

Cell Proliferation Assay^[1]

Cell Line:	OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells
Concentration:	6.0-48.0 μM
Incubation Time:	72 hours
Result:	Inhibited the proliferation of human cancer cells.

Western Blot Analysis^[1]

Cell Line:	OVCAR-8 or PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	6 hours
Result:	A concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	OVCAR-8 cells and PC3 cells
Concentration:	0 μM, 6 μM, 12 μM, and 18 μM
Incubation Time:	72 hours
Result:	Led to dose dependent increase of apoptosis.

体内研究
(In Vivo)

Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing^[1].

Animal Model:	Seven-week-old specific pathogen-free female Kunming mice (18-22 g)^[1]
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hours
Result:	Increased in blood glucose levels.

分子量

324.37

性状

Solid

Formula

C₂₀H₂₀O₄

CAS 号

20784-50-3

中文名称

补骨脂乙素；补骨酯乙素；异补骨脂查耳酮；异补骨脂查尔酮

结构分类

Flavonoids
Chalcones

Phenols
Polyphenols

来源

Plants
Leguminosae
Psoralea corylifoliaL.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 11 mg/mL(33.91 mM;Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.0829 mL	15.4145 mL	30.8290 mL
5 mM	0.6166 mL	3.0829 mL	6.1658 mL
10 mM	0.3083 mL	1.5414 mL	3.0829 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。