DER_PSEAE
ID DER_PSEAE Reviewed; 493 AA.
AC Q9HXJ8;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=GTPase Der {ECO:0000255|HAMAP-Rule:MF_00195};
DE AltName: Full=GTP-binding protein EngA {ECO:0000255|HAMAP-Rule:MF_00195};
GN Name=der {ECO:0000255|HAMAP-Rule:MF_00195}; Synonyms=engA;
GN OrderedLocusNames=PA3799;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: GTPase that plays an essential role in the late steps of
CC ribosome biogenesis. {ECO:0000255|HAMAP-Rule:MF_00195}.
CC -!- SUBUNIT: Associates with the 50S ribosomal subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngA (Der) GTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
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DR EMBL; AE004091; AAG07186.1; -; Genomic_DNA.
DR PIR; B83171; B83171.
DR RefSeq; NP_252488.1; NC_002516.2.
DR RefSeq; WP_003092786.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; Q9HXJ8; -.
DR SMR; Q9HXJ8; -.
DR STRING; 287.DR97_4070; -.
DR PaxDb; Q9HXJ8; -.
DR PRIDE; Q9HXJ8; -.
DR EnsemblBacteria; AAG07186; AAG07186; PA3799.
DR GeneID; 878229; -.
DR KEGG; pae:PA3799; -.
DR PATRIC; fig|208964.12.peg.3978; -.
DR PseudoCAP; PA3799; -.
DR HOGENOM; CLU_016077_6_2_6; -.
DR InParanoid; Q9HXJ8; -.
DR OMA; KFRFLEY; -.
DR PhylomeDB; Q9HXJ8; -.
DR BioCyc; PAER208964:G1FZ6-3870-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00195; GTPase_Der; 1.
DR InterPro; IPR031166; G_ENGA.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR016484; GTP-bd_EngA.
DR InterPro; IPR032859; KH_dom-like.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR Pfam; PF14714; KH_dom-like; 1.
DR Pfam; PF01926; MMR_HSR1; 2.
DR PIRSF; PIRSF006485; GTP-binding_EngA; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03594; GTPase_EngA; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 2.
DR PROSITE; PS51712; G_ENGA; 2.
PE 3: Inferred from homology;
KW GTP-binding; Nucleotide-binding; Reference proteome; Repeat;
KW Ribosome biogenesis.
FT CHAIN 1..493
FT /note="GTPase Der"
FT /id="PRO_0000179030"
FT DOMAIN 3..166
FT /note="EngA-type G 1"
FT DOMAIN 198..371
FT /note="EngA-type G 2"
FT DOMAIN 372..456
FT /note="KH-like"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT REGION 166..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 454..493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 468..493
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 9..16
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 56..60
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 118..121
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 204..211
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 251..255
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 316..319
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
SQ SEQUENCE 493 AA; 55006 MW; 6E65A3084939F7A0 CRC64;
MVPVIALVGR PNVGKSTLFN RLTKSRDAIV AEYAGLTRDR QYGEARWQGR TYIVIDTGGI
SGDEEGIDAK MAEQSLQAIE EADAVLFLVD SRAGMTAADQ MIAEHLRKRN KRSFLIANKV
DTIDPDLARA EFSPLGLGDA LPIAAAHGRG INHMLQEALG IFPKDNAEEE GEGEPASEEV
AEGEEPTRIP GPSEKDGIKI AIIGRPNVGK STLVNRMLGE ERVIVYDQAG TTRDSIYIPF
ERNEEKYTLI DTAGVRRRGK IFEAVEKFSV VKTLQAIQDA NVVIFVMDAR EGVVEHDLNL
LGFVLETGRA LVIALNKWDG MEAAERDYVK TELERRLLFV DFADIHFISA LHGTGVGHLY
KSVQESFRSA VTRWPTSRLT SILEDAVQVH QPPMVNGRRI KLRYAHLGGA NPPLIVIHGN
QVDAVPKAYT RYLEKTYRRV LKLVGTPIRI EYKGGENPYE GKKNSLTARQ VNKKRRLMSH
HKKAEKKKKD KRR
