DER_VIBCM
ID DER_VIBCM Reviewed; 494 AA.
AC C3LT16;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=GTPase Der {ECO:0000255|HAMAP-Rule:MF_00195};
DE AltName: Full=GTP-binding protein EngA {ECO:0000255|HAMAP-Rule:MF_00195};
GN Name=der {ECO:0000255|HAMAP-Rule:MF_00195}; Synonyms=engA;
GN OrderedLocusNames=VCM66_0721;
OS Vibrio cholerae serotype O1 (strain M66-2).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=579112;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M66-2;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: GTPase that plays an essential role in the late steps of
CC ribosome biogenesis. {ECO:0000255|HAMAP-Rule:MF_00195}.
CC -!- SUBUNIT: Associates with the 50S ribosomal subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngA (Der) GTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
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DR EMBL; CP001233; ACP05042.1; -; Genomic_DNA.
DR RefSeq; WP_000249403.1; NC_012578.1.
DR AlphaFoldDB; C3LT16; -.
DR SMR; C3LT16; -.
DR EnsemblBacteria; ACP05042; ACP05042; VCM66_0721.
DR GeneID; 57739473; -.
DR KEGG; vcm:VCM66_0721; -.
DR HOGENOM; CLU_016077_5_1_6; -.
DR OMA; KFRFLEY; -.
DR Proteomes; UP000001217; Chromosome I.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00195; GTPase_Der; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR031166; G_ENGA.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR016484; GTP-bd_EngA.
DR InterPro; IPR032859; KH_dom-like.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR Pfam; PF14714; KH_dom-like; 1.
DR Pfam; PF01926; MMR_HSR1; 2.
DR PIRSF; PIRSF006485; GTP-binding_EngA; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03594; GTPase_EngA; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 2.
DR PROSITE; PS51712; G_ENGA; 2.
PE 3: Inferred from homology;
KW GTP-binding; Nucleotide-binding; Repeat; Ribosome biogenesis.
FT CHAIN 1..494
FT /note="GTPase Der"
FT /id="PRO_1000124381"
FT DOMAIN 3..166
FT /note="EngA-type G 1"
FT DOMAIN 206..379
FT /note="EngA-type G 2"
FT DOMAIN 380..464
FT /note="KH-like"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 9..16
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 56..60
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 118..121
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 212..219
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 259..263
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 324..327
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
SQ SEQUENCE 494 AA; 55638 MW; A6F0020F23CE7645 CRC64;
MVPVVALVGR PNVGKSTLFN RLTRTRDALV ADFPGLTRDR KYGQAKLGEH EFIVIDTGGI
DGSEEGVETK MAQQSLAAID EADVVLFMVD GRAGLTVADE AIAQHLRRIE KPAILVVNKV
DGIDADAASA EFWQLGMDQM YQIAAAHGRG VGALIDRVLN PFAEQMESEQ AQLEDLTNEE
DPEEEQLEYS EEEAEAEYKR LQDLPIKLAI IGRPNVGKST LTNRILGEER VVVYDMPGTT
RDSIYIPMKR DEREYVLIDT AGVRRRKRIN ETVEKFSVVK TLQAIEDANV VLLVVDAREN
ISDQDLSLLG FALNSGRSIV IAVNKWDGLS FDVKEHVKKE LDRRLGFVDF ARIHFISALH
GTGVGHLFES VQEAYRSATT RVGTSVLTRI MKMATDDHQP PMVRGRRVKL KYAHAGGYNP
PIIVIHGNQV NELPDSYKRY LMNYYRKSLE IMGTPIRIQF QNSENPFEGK TNKMTLSQER
QRKRLMSMVK NRRK
