DES1L_ORYSJ
ID DES1L_ORYSJ Reviewed; 328 AA.
AC Q6H5U3; A0A0P0VMI9;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Sphingolipid delta(4)-desaturase DES1-like;
DE EC=1.14.19.17 {ECO:0000250|UniProtKB:Q9ZPH4};
GN OrderedLocusNames=Os02g0639600, LOC_Os02g42660;
GN ORFNames=OsJ_07673, OSJNBa0014E22.44, P0010C01.20;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Sphingolipid-delta-4-desaturase required for the biosynthesis
CC of delta-4-unsaturated sphingolipids and derivatives. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + 2 H(+) + O2
CC = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O;
CC Xref=Rhea:RHEA:46544, Rhea:RHEA-COMP:10438, Rhea:RHEA-COMP:10439,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:29033, ChEBI:CHEBI:29034, ChEBI:CHEBI:31488,
CC ChEBI:CHEBI:52639; EC=1.14.19.17;
CC Evidence={ECO:0000250|UniProtKB:Q9ZPH4};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family. DEGS
CC subfamily. {ECO:0000305}.
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DR EMBL; AP004768; BAD25351.1; -; Genomic_DNA.
DR EMBL; AP005513; BAD25906.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09454.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79967.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57453.1; -; Genomic_DNA.
DR EMBL; AK101968; BAG95317.1; -; mRNA.
DR RefSeq; XP_015623789.1; XM_015768303.1.
DR AlphaFoldDB; Q6H5U3; -.
DR STRING; 4530.OS02T0639600-01; -.
DR PaxDb; Q6H5U3; -.
DR PRIDE; Q6H5U3; -.
DR EnsemblPlants; Os02t0639600-01; Os02t0639600-01; Os02g0639600.
DR GeneID; 4330104; -.
DR Gramene; Os02t0639600-01; Os02t0639600-01; Os02g0639600.
DR KEGG; osa:4330104; -.
DR eggNOG; KOG2987; Eukaryota.
DR HOGENOM; CLU_032156_0_0_1; -.
DR InParanoid; Q6H5U3; -.
DR OMA; FTYIHLL; -.
DR OrthoDB; 1255438at2759; -.
DR PlantReactome; R-OSA-1119325; Sphingolipid metabolism.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6H5U3; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042284; F:sphingolipid delta-4 desaturase activity; IBA:GO_Central.
DR GO; GO:0046513; P:ceramide biosynthetic process; IBA:GO_Central.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd03508; Delta4-sphingolipid-FADS-like; 1.
DR InterPro; IPR011388; DES1/DES2.
DR InterPro; IPR005804; FA_desaturase_dom.
DR InterPro; IPR013866; Sphingolipid_d4-desaturase_N.
DR Pfam; PF00487; FA_desaturase; 1.
DR Pfam; PF08557; Lipid_DES; 1.
DR PIRSF; PIRSF017228; Sphnglp_dlt4_des; 1.
DR SMART; SM01269; Lipid_DES; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..328
FT /note="Sphingolipid delta(4)-desaturase DES1-like"
FT /id="PRO_0000430304"
FT TRANSMEM 50..70
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 98..102
FT /note="Histidine box-1"
FT /evidence="ECO:0000305"
FT MOTIF 135..139
FT /note="Histidine box-2"
FT /evidence="ECO:0000305"
FT MOTIF 266..270
FT /note="Histidine box-3"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 37869 MW; 98821042BDC2DFFB CRC64;
MGAAAGDGRE EEGVMATDFF WSYTDEPHAT RRREILAKHP QIKELFGPDP LAFLKIAAVV
SLQLWTATLL RDASWVKILT VAYFFGSFLN HNLFLAIHEL SHNLAFTTPS YNRWLGIFAN
LPIGVPMSIT FQKYHLEHHR FQGVDGIDMD IPSQAEAHAV KNTLSKSVWV VFQLFFYALR
PLFLKPKPPG LWEFTNLIIQ IALDASMVYF FGWKSLAYLI LSTFVGGGMH PMAGHFISEH
YVFNPDQETY SYYGPLNLMT WHVGYHNEHH DFPRIPGTRL YKVREIAPEY YNNLKSYKSW
SQVIYMYIMD QTVGPFSRMK RKAPKKDS
