DESI1_CAEEL
ID DESI1_CAEEL Reviewed; 334 AA.
AC H2KZK4; Q8MNS8;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Desumoylating isopeptidase 1 homolog;
DE Short=DeSI-1;
DE EC=3.4.-.-;
DE AltName: Full=Polyubiquitinated substrate transporter {ECO:0000303|PubMed:29666234};
DE Short=POST {ECO:0000303|PubMed:29666234};
GN ORFNames=F36D4.5 {ECO:0000312|WormBase:F36D4.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=29666234; DOI=10.1073/pnas.1711017115;
RA Hirayama S., Sugihara M., Morito D., Iemura S.I., Natsume T., Murata S.,
RA Nagata K.;
RT "Nuclear export of ubiquitinated proteins via the UBIN-POST system.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E4199-E4208(2018).
CC -!- FUNCTION: Protease which deconjugates SUMO from some substrate
CC proteins. Has isopeptidase but not SUMO-processing activity (By
CC similarity). Collaborates with ubql-1 in the export of ubiquitinated
CC proteins from the nucleus to the cytoplasm (PubMed:29666234).
CC {ECO:0000250|UniProtKB:Q9CQT7, ECO:0000269|PubMed:29666234}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6ICB0}. Nucleus
CC {ECO:0000250|UniProtKB:Q6ICB0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=H2KZK4-1; Sequence=Displayed;
CC Name=b;
CC IsoId=H2KZK4-2; Sequence=VSP_060089;
CC -!- TISSUE SPECIFICITY: Expressed in the pharynx, hypodermis, intestine,
CC head neuron and tail neuron. {ECO:0000269|PubMed:29666234}.
CC -!- DISRUPTION PHENOTYPE: Worms lacking both POST and ubql-1 have a shorter
CC lifespan and display an accumulation of ubiquitinated proteins in the
CC nucleus. {ECO:0000269|PubMed:29666234}.
CC -!- SIMILARITY: Belongs to the DeSI family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284605; CCD68607.1; -; Genomic_DNA.
DR EMBL; BX284605; CCD68608.1; -; Genomic_DNA.
DR RefSeq; NP_741591.1; NM_171506.1. [H2KZK4-1]
DR RefSeq; NP_741592.1; NM_171507.4. [H2KZK4-2]
DR AlphaFoldDB; H2KZK4; -.
DR SMR; H2KZK4; -.
DR STRING; 6239.F36D4.5a; -.
DR EPD; H2KZK4; -.
DR PaxDb; H2KZK4; -.
DR PeptideAtlas; H2KZK4; -.
DR EnsemblMetazoa; F36D4.5a.1; F36D4.5a.1; WBGene00018090. [H2KZK4-1]
DR EnsemblMetazoa; F36D4.5a.2; F36D4.5a.2; WBGene00018090. [H2KZK4-1]
DR EnsemblMetazoa; F36D4.5a.3; F36D4.5a.3; WBGene00018090. [H2KZK4-1]
DR EnsemblMetazoa; F36D4.5b.1; F36D4.5b.1; WBGene00018090. [H2KZK4-2]
DR EnsemblMetazoa; F36D4.5b.2; F36D4.5b.2; WBGene00018090. [H2KZK4-2]
DR EnsemblMetazoa; F36D4.5b.3; F36D4.5b.3; WBGene00018090. [H2KZK4-2]
DR EnsemblMetazoa; F36D4.5b.4; F36D4.5b.4; WBGene00018090. [H2KZK4-2]
DR GeneID; 179324; -.
DR KEGG; cel:CELE_F36D4.5; -.
DR UCSC; F36D4.5b.1; c. elegans.
DR CTD; 179324; -.
DR WormBase; F36D4.5a; CE28391; WBGene00018090; -. [H2KZK4-1]
DR WormBase; F36D4.5b; CE30778; WBGene00018090; -. [H2KZK4-2]
DR eggNOG; KOG0324; Eukaryota.
DR GeneTree; ENSGT00940000171392; -.
DR HOGENOM; CLU_076704_0_0_1; -.
DR InParanoid; H2KZK4; -.
DR OMA; SNSDRIC; -.
DR OrthoDB; 1083890at2759; -.
DR PhylomeDB; H2KZK4; -.
DR PRO; PR:H2KZK4; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00018090; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0101005; F:deubiquitinase activity; IBA:GO_Central.
DR GO; GO:0016579; P:protein deubiquitination; IBA:GO_Central.
DR GO; GO:0070646; P:protein modification by small protein removal; IBA:GO_Central.
DR Gene3D; 3.90.1720.30; -; 1.
DR InterPro; IPR008580; PPPDE_dom.
DR InterPro; IPR042266; PPPDE_sf.
DR PANTHER; PTHR12378; PTHR12378; 1.
DR Pfam; PF05903; Peptidase_C97; 1.
DR SMART; SM01179; DUF862; 1.
DR PROSITE; PS51858; PPPDE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Hydrolase; Nucleus; Protease;
KW Reference proteome.
FT CHAIN 1..334
FT /note="Desumoylating isopeptidase 1 homolog"
FT /id="PRO_0000446645"
FT DOMAIN 30..174
FT /note="PPPDE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT REGION 310..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 55
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT ACT_SITE 133
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT VAR_SEQ 1..19
FT /note="Missing (in isoform b)"
FT /id="VSP_060089"
SQ SEQUENCE 334 AA; 36408 MW; 897567EAF1B57958 CRC64;
MLTDLFYSTF GCLYSPTSTM DVMGTARRKT VVRLNVYDMY WLNDYASNIG VGIFHSGIEV
FGVEYAYGGH PYQFSGVFEN SPQDAEELGE TFKFKESIVV GETERSTSDI RKLIKSLGED
FRGDRYHLIS RNCNHFSAVL ARELTGKDIP GWINRLANLS GSIPFLEKCI PQEWLTPIVL
QASVDEKKRG SVDSAEEATE KLVVRSLNDS RTTILDNRTA NGAIIMSASS SNSDRICMSP
SSSSSASSCD TLDYDDLIVQ TPSTFSSEKK SRSNSPPIFR IWNTIKATIN GTQQTAPTGA
ATVIPASSAS NIGKTNSTPG TTSNGLAKPT CSEC
