ID   DESI1_RAT               Reviewed;         168 AA.
AC   Q4KM30;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Desumoylating isopeptidase 1;
DE            Short=DeSI-1;
DE            EC=3.4.-.-;
DE   AltName: Full=PPPDE peptidase domain-containing protein 2;
GN   Name=Desi1; Synonyms=Fam152b, Pppde2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Protease which deconjugates SUMO1, SUMO2 and SUMO3 from some
CC       substrate proteins. Has isopeptidase but not SUMO-processing activity.
CC       Desumoylates ZBTB46 (By similarity). Collaborates with UBQLN4 in the
CC       export of ubiquitinated proteins from the nucleus to the cytoplasm (By
CC       similarity). {ECO:0000250|UniProtKB:Q6ICB0,
CC       ECO:0000250|UniProtKB:Q9CQT7}.
CC   -!- SUBUNIT: Homodimer (By similarity). Interacts with UBQLN4; leading to
CC       the export of UBQLN4 from the nucleus (By similarity).
CC       {ECO:0000250|UniProtKB:Q6ICB0, ECO:0000250|UniProtKB:Q9CQT7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6ICB0}. Nucleus
CC       {ECO:0000250|UniProtKB:Q6ICB0}. Note=Shuttles between the nucleus and
CC       the cytoplasm; exported from the nucleus in a XPO1/CRM1-dependent
CC       manner via its nuclear export signal motifs.
CC       {ECO:0000250|UniProtKB:Q6ICB0}.
CC   -!- SIMILARITY: Belongs to the DeSI family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC098857; AAH98857.1; -; mRNA.
DR   RefSeq; NP_001020874.1; NM_001025703.1.
DR   AlphaFoldDB; Q4KM30; -.
DR   SMR; Q4KM30; -.
DR   STRING; 10116.ENSRNOP00000007424; -.
DR   MEROPS; C97.001; -.
DR   PaxDb; Q4KM30; -.
DR   PRIDE; Q4KM30; -.
DR   GeneID; 315160; -.
DR   KEGG; rno:315160; -.
DR   CTD; 27351; -.
DR   RGD; 1305776; Desi1.
DR   VEuPathDB; HostDB:ENSRNOG00000005577; -.
DR   eggNOG; KOG0324; Eukaryota.
DR   HOGENOM; CLU_101028_0_0_1; -.
DR   InParanoid; Q4KM30; -.
DR   OMA; LFDNNCN; -.
DR   OrthoDB; 1214552at2759; -.
DR   PhylomeDB; Q4KM30; -.
DR   PRO; PR:Q4KM30; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000005577; Expressed in testis and 19 other tissues.
DR   Genevisible; Q4KM30; RN.
DR   GO; GO:0005829; C:cytosol; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR   GO; GO:0016929; F:deSUMOylase activity; ISO:RGD.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0061676; F:importin-alpha family protein binding; ISO:RGD.
DR   GO; GO:0016926; P:protein desumoylation; ISO:RGD.
DR   GO; GO:0006611; P:protein export from nucleus; ISO:RGD.
DR   GO; GO:0070646; P:protein modification by small protein removal; IBA:GO_Central.
DR   GO; GO:0032434; P:regulation of proteasomal ubiquitin-dependent protein catabolic process; ISO:RGD.
DR   Gene3D; 3.90.1720.30; -; 1.
DR   InterPro; IPR008580; PPPDE_dom.
DR   InterPro; IPR042266; PPPDE_sf.
DR   PANTHER; PTHR12378; PTHR12378; 1.
DR   Pfam; PF05903; Peptidase_C97; 1.
DR   SMART; SM01179; DUF862; 1.
DR   PROSITE; PS51858; PPPDE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Nucleus; Protease; Reference proteome.
FT   CHAIN           1..168
FT                   /note="Desumoylating isopeptidase 1"
FT                   /id="PRO_0000318152"
FT   DOMAIN          7..149
FT                   /note="PPPDE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT   MOTIF           83..91
FT                   /note="Nuclear export signal 1"
FT                   /evidence="ECO:0000250|UniProtKB:Q6ICB0"
FT   MOTIF           139..153
FT                   /note="Nuclear export signal 2"
FT                   /evidence="ECO:0000250|UniProtKB:Q6ICB0"
FT   ACT_SITE        38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT   ACT_SITE        108
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
SQ   SEQUENCE   168 AA;  18385 MW;  5C81CE4CE5752421 CRC64;
     MEPPNLYPVK LYVYDLSKGL ARRLSPIMLG KQLEGIWHTS IVVHKDEFFF GSSGISSCTP
     GGTLLGPPDS VVDVGNTEVT EEIFLEYLSS LGESLFRGEA YNLFEHNCNT FSNEVAQFLT
     GRKIPSYITD LPSEVLSTPF GQALRPFLDS IQIQPPGGNS VGRPNGQS