ID   DESI2_CHICK             Reviewed;         193 AA.
AC   Q5ZIV7;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Deubiquitinase DESI2 {ECO:0000250|UniProtKB:Q9BSY9};
DE            EC=3.4.19.12 {ECO:0000250|UniProtKB:Q9BSY9};
DE   AltName: Full=Desumoylating isopeptidase 2;
DE            Short=DeSI-2;
DE   AltName: Full=PPPDE peptidase domain-containing protein 1;
DE   AltName: Full=Protein FAM152A;
GN   Name=DESI2; Synonyms=FAM152A, PPPDE1; ORFNames=RCJMB04_23d23;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Has deubiquitinating activity towards 'Lys-48'- and 'Lys-63'-
CC       linked polyubiquitin chains. {ECO:0000250|UniProtKB:Q9BSY9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q9BSY9};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9D291}.
CC   -!- SIMILARITY: Belongs to the DeSI family. {ECO:0000305}.
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DR   EMBL; AJ720677; CAG32336.1; -; mRNA.
DR   RefSeq; NP_001008460.1; NM_001008460.1.
DR   AlphaFoldDB; Q5ZIV7; -.
DR   SMR; Q5ZIV7; -.
DR   STRING; 9031.ENSGALP00000017350; -.
DR   MEROPS; C97.002; -.
DR   PaxDb; Q5ZIV7; -.
DR   Ensembl; ENSGALT00000017370; ENSGALP00000017350; ENSGALG00000010678.
DR   GeneID; 421494; -.
DR   KEGG; gga:421494; -.
DR   CTD; 51029; -.
DR   VEuPathDB; HostDB:geneid_421494; -.
DR   eggNOG; KOG0324; Eukaryota.
DR   GeneTree; ENSGT00730000111005; -.
DR   HOGENOM; CLU_069001_5_1_1; -.
DR   InParanoid; Q5ZIV7; -.
DR   OMA; GVYWTRP; -.
DR   OrthoDB; 1300278at2759; -.
DR   PhylomeDB; Q5ZIV7; -.
DR   TreeFam; TF313188; -.
DR   PRO; PR:Q5ZIV7; -.
DR   Proteomes; UP000000539; Chromosome 3.
DR   Bgee; ENSGALG00000010678; Expressed in spermatocyte and 12 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0101005; F:deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0016579; P:protein deubiquitination; IBA:GO_Central.
DR   GO; GO:0070646; P:protein modification by small protein removal; IBA:GO_Central.
DR   Gene3D; 3.90.1720.30; -; 1.
DR   InterPro; IPR008580; PPPDE_dom.
DR   InterPro; IPR042266; PPPDE_sf.
DR   PANTHER; PTHR12378; PTHR12378; 1.
DR   Pfam; PF05903; Peptidase_C97; 1.
DR   SMART; SM01179; DUF862; 1.
DR   PROSITE; PS51858; PPPDE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Protease; Reference proteome;
KW   Ubl conjugation pathway.
FT   CHAIN           1..193
FT                   /note="Deubiquitinase DESI2"
FT                   /id="PRO_0000317726"
FT   DOMAIN          4..148
FT                   /note="PPPDE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT   REGION          165..193
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        176..193
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        29
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01205"
FT   ACT_SITE        107
FT                   /evidence="ECO:0000250|UniProtKB:Q9BSY9,
FT                   ECO:0000255|PROSITE-ProRule:PRU01205"
SQ   SEQUENCE   193 AA;  21409 MW;  28DD7155F9AE0EB7 CRC64;
     MANQLVILNV YDMYWMNEYT SSLGIGVFHS GIEVYGREFA YGGHPYPFSG IFEISPGNAS
     ELGETFKFKE AVVLGSTDFM EDDIEKIVEE LGKEFKGNAY HLMHKNCNHF SSALSEILCG
     KEIPRWVNRL AYFSSCIPFL QSCLPKEWLT PAALQSSVSQ ELQDELEEAE DAAASASLAS
     SASGSRTGRH TKL