DESR_BACSU
ID DESR_BACSU Reviewed; 199 AA.
AC O34723; Q796C7;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Transcriptional regulatory protein DesR;
GN Name=desR; Synonyms=yocG; OrderedLocusNames=BSU19200;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequence analysis of the Bacillus subtilis chromosome region between the
RT terC and odhAB loci cloned in a yeast artificial chromosome.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RX PubMed=11285232; DOI=10.1093/emboj/20.7.1681;
RA Aguilar P.S., Hernandez-Arriaga A.M., Cybulski L.E., Erazo A.C.,
RA de Mendoza D.;
RT "Molecular basis of thermosensing: a two-component signal transduction
RT thermometer in Bacillus subtilis.";
RL EMBO J. 20:1681-1691(2001).
RN [4]
RP FUNCTION, AND GENE NAME.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [5]
RP FUNCTION.
RX PubMed=12207704; DOI=10.1046/j.1365-2958.2002.03103.x;
RA Cybulski L.E., Albanesi D., Mansilla M.C., Altabe S., Aguilar P.S.,
RA de Mendoza D.;
RT "Mechanism of membrane fluidity optimization: isothermal control of the
RT Bacillus subtilis acyl-lipid desaturase.";
RL Mol. Microbiol. 45:1379-1388(2002).
RN [6]
RP FUNCTION.
RX PubMed=14734164; DOI=10.1016/s0378-1097(03)00852-8;
RA Hunger K., Beckering C.L., Marahiel M.A.;
RT "Genetic evidence for the temperature-sensing ability of the membrane
RT domain of the Bacillus subtilis histidine kinase DesK.";
RL FEMS Microbiol. Lett. 230:41-46(2004).
RN [7]
RP FUNCTION.
RX PubMed=15090506; DOI=10.1128/jb.186.9.2655-2663.2004;
RA Albanesi D., Mansilla M.C., de Mendoza D.;
RT "The membrane fluidity sensor DesK of Bacillus subtilis controls the signal
RT decay of its cognate response regulator.";
RL J. Bacteriol. 186:2655-2663(2004).
RN [8]
RP REVIEW.
RX PubMed=15711796; DOI=10.1007/s00203-005-0759-8;
RA Mansilla M.C., de Mendoza D.;
RT "The Bacillus subtilis desaturase: a model to understand phospholipid
RT modification and temperature sensing.";
RL Arch. Microbiol. 183:229-235(2005).
CC -!- FUNCTION: Member of the two-component regulatory system DesR/DesK,
CC responsible for cold induction of the des gene coding for the Delta5
CC acyl-lipid desaturase. {ECO:0000269|PubMed:11285232,
CC ECO:0000269|PubMed:11717295, ECO:0000269|PubMed:12207704,
CC ECO:0000269|PubMed:14734164, ECO:0000269|PubMed:15090506}.
CC -!- INTERACTION:
CC O34723; O34757: desK; NbExp=3; IntAct=EBI-15806271, EBI-15806221;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by DesK. {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF027868; AAB84438.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13812.1; -; Genomic_DNA.
DR PIR; D69901; D69901.
DR RefSeq; NP_389801.1; NC_000964.3.
DR RefSeq; WP_004399356.1; NZ_JNCM01000036.1.
DR PDB; 4LDZ; X-ray; 2.31 A; A/B=1-199.
DR PDB; 4LE0; X-ray; 2.27 A; A/B=1-135.
DR PDB; 4LE1; X-ray; 1.95 A; A/B=1-135.
DR PDB; 4LE2; X-ray; 2.54 A; A/B/C/D=1-135.
DR PDB; 5IUJ; X-ray; 3.20 A; C/F=1-135.
DR PDB; 5IUK; X-ray; 2.90 A; C/F=1-135.
DR PDB; 5IUL; X-ray; 3.15 A; C/F=1-135.
DR PDB; 5IUN; X-ray; 2.79 A; C/D/F=1-135.
DR PDBsum; 4LDZ; -.
DR PDBsum; 4LE0; -.
DR PDBsum; 4LE1; -.
DR PDBsum; 4LE2; -.
DR PDBsum; 5IUJ; -.
DR PDBsum; 5IUK; -.
DR PDBsum; 5IUL; -.
DR PDBsum; 5IUN; -.
DR AlphaFoldDB; O34723; -.
DR SMR; O34723; -.
DR DIP; DIP-48967N; -.
DR IntAct; O34723; 1.
DR STRING; 224308.BSU19200; -.
DR PaxDb; O34723; -.
DR EnsemblBacteria; CAB13812; CAB13812; BSU_19200.
DR GeneID; 939664; -.
DR KEGG; bsu:BSU19200; -.
DR PATRIC; fig|224308.179.peg.2098; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; O34723; -.
DR OMA; LDRFAPM; -.
DR PhylomeDB; O34723; -.
DR BioCyc; BSUB:BSU19200-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..199
FT /note="Transcriptional regulatory protein DesR"
FT /id="PRO_0000360772"
FT DOMAIN 3..117
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 131..196
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 155..174
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:4LE1"
FT TURN 7..10
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 12..21
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 37..46
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 58..60
FT /evidence="ECO:0007829|PDB:4LE0"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:4LE1"
FT TURN 68..71
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 87..93
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 98..101
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 106..117
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:4LE1"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:4LE1"
FT HELIX 140..151
FT /evidence="ECO:0007829|PDB:4LDZ"
FT HELIX 155..161
FT /evidence="ECO:0007829|PDB:4LDZ"
FT HELIX 166..192
FT /evidence="ECO:0007829|PDB:4LDZ"
SQ SEQUENCE 199 AA; 22177 MW; 8D27B10320FA4974 CRC64;
MISIFIAEDQ QMLLGALGSL LNLEDDMEVV GKGTTGQDAV DFVKKRQPDV CIMDIEMPGK
TGLEAAEELK DTGCKIIILT TFARPGYFQR AIKAGVKGYL LKDSPSEELA NAIRSVMNGK
RIYAPELMED LYSEANPLTD REKEVLELVA DGKNTKEIAQ ELSIKSGTVR NYISMILEKL
EVKNRIEAIT RSKEKGWFK
