DET1_HUMAN
ID DET1_HUMAN Reviewed; 550 AA.
AC Q7L5Y6; B3KNN6; Q2VPC0; Q9NWD5;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=DET1 homolog;
DE AltName: Full=De-etiolated-1 homolog;
GN Name=DET1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo, and Neuroblastoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND IDENTIFICATION IN THE DCX DET1-COP1
RP COMPLEX WITH RBX1; CUL4A; COP1 AND DDB1.
RX PubMed=14739464; DOI=10.1126/science.1093549;
RA Wertz I.E., O'Rourke K.M., Zhang Z., Dornan D., Arnott D., Deshaies R.J.,
RA Dixit V.M.;
RT "Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin
RT ligase.";
RL Science 303:1371-1374(2004).
CC -!- FUNCTION: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex,
CC which is required for ubiquitination and subsequent degradation of
CC target proteins. The complex is involved in JUN ubiquitination and
CC degradation. {ECO:0000269|PubMed:14739464}.
CC -!- SUBUNIT: Component of the DCX DET1-COP1 ubiquitin ligase complex at
CC least composed of RBX1, DET1, DDB1, CUL4A and COP1.
CC {ECO:0000269|PubMed:14739464}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7L5Y6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7L5Y6-2; Sequence=VSP_043012;
CC -!- TISSUE SPECIFICITY: Highly expressed in the ovary, some lymphoid organs
CC and resting leukocytes. {ECO:0000269|PubMed:14739464}.
CC -!- SIMILARITY: Belongs to the DET1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA91447.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK000965; BAA91447.1; ALT_INIT; mRNA.
DR EMBL; AK054603; BAG51398.1; -; mRNA.
DR EMBL; BC001242; AAH01242.2; -; mRNA.
DR EMBL; BC109060; AAI09061.1; -; mRNA.
DR EMBL; BC109061; AAI09062.1; -; mRNA.
DR CCDS; CCDS45343.1; -. [Q7L5Y6-2]
DR CCDS; CCDS45344.1; -. [Q7L5Y6-1]
DR RefSeq; NP_001137546.1; NM_001144074.2. [Q7L5Y6-1]
DR RefSeq; NP_001308525.1; NM_001321596.1. [Q7L5Y6-1]
DR RefSeq; NP_060466.2; NM_017996.4. [Q7L5Y6-2]
DR AlphaFoldDB; Q7L5Y6; -.
DR BioGRID; 120387; 48.
DR CORUM; Q7L5Y6; -.
DR IntAct; Q7L5Y6; 39.
DR STRING; 9606.ENSP00000456340; -.
DR iPTMnet; Q7L5Y6; -.
DR PhosphoSitePlus; Q7L5Y6; -.
DR BioMuta; DET1; -.
DR DMDM; 55976471; -.
DR EPD; Q7L5Y6; -.
DR MassIVE; Q7L5Y6; -.
DR MaxQB; Q7L5Y6; -.
DR PaxDb; Q7L5Y6; -.
DR PeptideAtlas; Q7L5Y6; -.
DR PRIDE; Q7L5Y6; -.
DR ProteomicsDB; 68817; -. [Q7L5Y6-1]
DR ProteomicsDB; 68818; -. [Q7L5Y6-2]
DR Antibodypedia; 43705; 73 antibodies from 15 providers.
DR DNASU; 55070; -.
DR Ensembl; ENST00000268148.13; ENSP00000268148.8; ENSG00000140543.15. [Q7L5Y6-1]
DR Ensembl; ENST00000444300.1; ENSP00000408994.1; ENSG00000140543.15. [Q7L5Y6-2]
DR Ensembl; ENST00000564406.5; ENSP00000456340.1; ENSG00000140543.15. [Q7L5Y6-2]
DR GeneID; 55070; -.
DR KEGG; hsa:55070; -.
DR MANE-Select; ENST00000268148.13; ENSP00000268148.8; NM_001144074.3; NP_001137546.1.
DR UCSC; uc002bmq.3; human. [Q7L5Y6-1]
DR CTD; 55070; -.
DR DisGeNET; 55070; -.
DR GeneCards; DET1; -.
DR HGNC; HGNC:25477; DET1.
DR HPA; ENSG00000140543; Low tissue specificity.
DR MIM; 608727; gene.
DR neXtProt; NX_Q7L5Y6; -.
DR OpenTargets; ENSG00000140543; -.
DR PharmGKB; PA134943899; -.
DR VEuPathDB; HostDB:ENSG00000140543; -.
DR eggNOG; KOG2558; Eukaryota.
DR GeneTree; ENSGT00390000005224; -.
DR HOGENOM; CLU_036725_1_0_1; -.
DR InParanoid; Q7L5Y6; -.
DR OrthoDB; 603320at2759; -.
DR PhylomeDB; Q7L5Y6; -.
DR TreeFam; TF324729; -.
DR PathwayCommons; Q7L5Y6; -.
DR Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q7L5Y6; -.
DR SIGNOR; Q7L5Y6; -.
DR BioGRID-ORCS; 55070; 179 hits in 1090 CRISPR screens.
DR ChiTaRS; DET1; human.
DR GenomeRNAi; 55070; -.
DR Pharos; Q7L5Y6; Tbio.
DR PRO; PR:Q7L5Y6; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q7L5Y6; protein.
DR Bgee; ENSG00000140543; Expressed in hindlimb stylopod muscle and 106 other tissues.
DR ExpressionAtlas; Q7L5Y6; baseline and differential.
DR Genevisible; Q7L5Y6; HS.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0031464; C:Cul4A-RING E3 ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0031461; C:cullin-RING ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:UniProtKB.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IPI:UniProtKB.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IMP:UniProtKB.
DR GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IPI:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IPI:UniProtKB.
DR GO; GO:0065003; P:protein-containing complex assembly; IDA:UniProtKB.
DR InterPro; IPR019138; De-etiolated_protein_1_Det1.
DR PANTHER; PTHR13374; PTHR13374; 1.
DR Pfam; PF09737; Det1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..550
FT /note="DET1 homolog"
FT /id="PRO_0000129026"
FT VAR_SEQ 1
FT /note="M -> MVGQILKRDVIM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043012"
SQ SEQUENCE 550 AA; 63848 MW; A164AC844F897DF7 CRC64;
MDHHVSTIKP RRIQNQNVIH RLERRRISSG KAGTHWHQVR VFHQNVFPNF TVVNVEKPPC
FLRKFSPDGR YFIAFSSDQT SLEIYEYQGC QAAEDLLQGY EGEILSNGND QRSVNIRGRL
FERFFVLLHI TNVAANGEHL NRECSLFTDD CRCVIVGSAA YLPDEPHPPF FEVYRNSESV
TPNPRSPLED YSLHIIDLHT GRLCDTRTFK CDKVVLSHNQ GLYLYKNILA ILSVQQQTIH
VFQVTPEGTF IDVRTIGRFC YEDDLLTVSA VFPEVQRDSQ TGMANPFRDP FINSLKHRLL
VYLWRRAEQD GSAMAKRRFF QYFDQLRQLR MWKMQLLDEN HLFIKYTSED VVTLRVTDPS
QASFFVVYNM VTTEVIAVFE NTSDELLELF ENFCDLFRNA TLHSEVQFPC SASSNNFARQ
IQRRFKDTII NAKYGGHTEA VRRLLGQLPI SAQSYSGSPY LDLSLFSYDD KWVSVMERPK
TCGDHPIRFY ARDSGLLKFE IQAGLLGRPI NHTVRRLVAF TFHPFEPFAI SVQRTNAEYV
VNFHMRHCCT
