DET1_SOLLC
ID DET1_SOLLC Reviewed; 523 AA.
AC Q9ZNU6;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Light-mediated development protein DET1;
DE AltName: Full=Deetiolated1 homolog;
DE AltName: Full=High pigmentation protein 2;
DE AltName: Full=Protein dark green;
DE AltName: Full=tDET1;
GN Name=DET1; Synonyms=dg, hp2;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND MUTAGENESIS OF
RP 478-GLY--GLU-480 AND PRO-498.
RX PubMed=9927635; DOI=10.2307/3870847;
RA Mustilli A.C., Fenzi F., Ciliento R., Alfano F., Bowler C.;
RT "Phenotype of the tomato high pigment-2 mutant is caused by a mutation in
RT the tomato homolog of DEETIOLATED1.";
RL Plant Cell 11:145-157(1999).
RN [2]
RP MUTAGENESIS OF ASN-34.
RX PubMed=12589545; DOI=10.1007/s00122-002-1080-4;
RA Levin I., Frankel P., Gilboa N., Tanny S., Lalazar A.;
RT "The tomato dark green mutation is a novel allele of the tomato homolog of
RT the DEETIOLATED1 gene.";
RL Theor. Appl. Genet. 106:454-460(2003).
CC -!- FUNCTION: Component of light signal transduction machinery. Involved in
CC fruit pigmentation and fruit nutritional quality. Acts as a negative
CC regulator of fruit pigmentation. Probably acts by participating in the
CC CDD complex, a complex probably required to regulate the activity of
CC ubiquitin conjugating enzymes. Repression of photomorphogenesis is
CC probably mediated by ubiquitination and subsequent degradation of
CC photomorphogenesis-promoting factors such as HY5.
CC -!- SUBUNIT: Probable component of the CDD complex, which probably also
CC contains DDB1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9ZNU6; Q8LGH4: CUL4; Xeno; NbExp=2; IntAct=EBI-2029363, EBI-541750;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DET1 family. {ECO:0000305}.
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DR EMBL; AJ224356; CAA11914.1; -; Genomic_DNA.
DR EMBL; AJ224357; CAA11914.1; JOINED; Genomic_DNA.
DR EMBL; AJ222798; CAA10993.1; -; mRNA.
DR RefSeq; NP_001234148.1; NM_001247219.2.
DR AlphaFoldDB; Q9ZNU6; -.
DR IntAct; Q9ZNU6; 2.
DR STRING; 4081.Solyc01g056340.2.1; -.
DR PaxDb; Q9ZNU6; -.
DR PRIDE; Q9ZNU6; -.
DR EnsemblPlants; Solyc01g056340.3.1; Solyc01g056340.3.1; Solyc01g056340.3.
DR GeneID; 778329; -.
DR Gramene; Solyc01g056340.3.1; Solyc01g056340.3.1; Solyc01g056340.3.
DR KEGG; sly:778329; -.
DR eggNOG; KOG2558; Eukaryota.
DR HOGENOM; CLU_036725_2_0_1; -.
DR InParanoid; Q9ZNU6; -.
DR OMA; AFSYVFF; -.
DR OrthoDB; 603320at2759; -.
DR PhylomeDB; Q9ZNU6; -.
DR Proteomes; UP000004994; Chromosome 1.
DR ExpressionAtlas; Q9ZNU6; baseline.
DR GO; GO:0031461; C:cullin-RING ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0009585; P:red, far-red light phototransduction; IEA:UniProtKB-KW.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR019138; De-etiolated_protein_1_Det1.
DR PANTHER; PTHR13374; PTHR13374; 1.
DR Pfam; PF09737; Det1; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phytochrome signaling pathway; Reference proteome.
FT CHAIN 1..523
FT /note="Light-mediated development protein DET1"
FT /id="PRO_0000129029"
FT MOTIF 401..416
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 472..490
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MUTAGEN 34
FT /note="N->I: In dg; induces a much darker mature-green due
FT to higher total chlorophyll content."
FT /evidence="ECO:0000269|PubMed:12589545"
FT MUTAGEN 478..480
FT /note="Missing: In hp-2; induces higher fruit and doliage
FT pigmentation due to higher levels of carotenoids, primarily
FT lycopene, and flavonoids in the mature ripe-red fruit."
FT /evidence="ECO:0000269|PubMed:9927635"
FT MUTAGEN 498
FT /note="P->S: In hp-2j; induces higher fruit and doliage
FT pigmentation due to higher levels of carotenoids, primarily
FT lycopene, and flavonoids in the mature ripe-red fruit."
FT /evidence="ECO:0000269|PubMed:9927635"
SQ SEQUENCE 523 AA; 60418 MW; 24DAEE4F991EC5AB CRC64;
MFKTNNVTAR LFERQICTPA PGTSIHRARR FYENVVPSYT IYDVECPDHS FRKFTDDGLY
FVSFSRNHQD LVVYRPTWLT FSCKEEDCDT HDLPLKARKF ESFFTQLYSV TLASSGELIC
KDFFLYMESN QFGLFATSTA QIHDAPPTGG AIQGVPSVEK ITFHLLRLVD GAILDERVFH
NDYVNLAHSI GAFLYDDLLA IVSLRYQRIH ILQIRDSGDL VDVRAIGEFC REDDELFLNS
NSQVLVNHVG NGFHHSLPQS ETSFLSGIKQ RLLSYIFRGI WNEADQTMRV QCLKKKFYFH
FQDYIDLIIW KVQFLDRHHL LIKFGSVDGG VSRNADIHPS FFAVYNMETT EIVAFYQNSA
DELYFLFELF SDHFHVSSKS SLHMNFMSSH SNNIHALEQL RCTKNKATNF SQFVKKMMAS
LPCSCQSQSP SPYFDQSLFR FDEKLISAID RHRQSTDHPI KFISRRQPNI LKFKMKPGPE
AGSTDGRTKK ICSFLFHPIL PLALSVQQTL FLQASVVNIH FRR
