DET2_BRADI
ID DET2_BRADI Reviewed; 271 AA.
AC I1HTF7;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 25-MAY-2022, entry version 51.
DE RecName: Full=Protein DEETIOLATED 2 {ECO:0000305};
DE Short=BdDET2 {ECO:0000303|PubMed:32146811};
DE AltName: Full=(22S,24R)-22-hydroxy-5alpha-ergostan-3-one synthase {ECO:0000305};
DE EC=1.3.1.22 {ECO:0000269|PubMed:32146811};
DE AltName: Full=Campest-3-one synthase {ECO:0000305};
DE EC=1.3.1.22 {ECO:0000269|PubMed:32146811};
DE AltName: Full=Steroid 5-alpha-reductase DET2 {ECO:0000305};
DE EC=1.3.1.22 {ECO:0000269|PubMed:32146811};
GN Name=DET2 {ECO:0000303|PubMed:32146811};
GN OrderedLocusNames=BRADI_2g55110v3 {ECO:0000312|EMBL:KQK10601.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bd21;
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=32146811; DOI=10.1021/acs.jafc.9b07963;
RA Roh J., Moon J., Youn J.-H., Seo C., Park Y.J., Kim S.-K.;
RT "Establishment of biosynthetic pathways to generate castasterone as the
RT biologically active brassinosteroid in Brachypodium distachyon.";
RL J. Agric. Food Chem. 68:3912-3923(2020).
CC -!- FUNCTION: Involved in reduction steps of the biosynthesis of plant
CC campesterol-derivative steroids, ending to castasterone (CS) but
CC missing brassinolide (BL) (PubMed:32146811). Catalyzes the conversion
CC of campest-4-en-3-one (campesta-4-en-3-one, 4-en-3-one) to campest-3-
CC one (campesta-3-one, 3-one) and of (22S,24R)-22-hydroxyergost-4-en-3-
CC one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-one) to (22S,24R)-22-
CC hydroxy-5alpha-ergostan-3-one (22-hydroxy-campesta-3-one, 22-OH-3-one)
CC (PubMed:32146811). {ECO:0000269|PubMed:32146811}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid +
CC H(+) + NADPH; Xref=Rhea:RHEA:54384, ChEBI:CHEBI:13601,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47909, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-campestan-3-one + NADP(+) = campest-4-en-3-one + H(+) +
CC NADPH; Xref=Rhea:RHEA:54416, ChEBI:CHEBI:15378, ChEBI:CHEBI:18533,
CC ChEBI:CHEBI:18534, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:54418;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(22S,24R)-22-hydroxy-5alpha-ergostan-3-one + NADP(+) = (22S)-
CC 22-hydroxycampest-4-en-3-one + H(+) + NADPH; Xref=Rhea:RHEA:69987,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:59411, ChEBI:CHEBI:72330;
CC Evidence={ECO:0000269|PubMed:32146811};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:69989;
CC Evidence={ECO:0000269|PubMed:32146811};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000269|PubMed:32146811}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
CC {ECO:0000305}.
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DR EMBL; CM000881; KQK10601.1; -; Genomic_DNA.
DR RefSeq; XP_003567273.1; XM_003567225.3.
DR STRING; 15368.BRADI2G55110.1; -.
DR EnsemblPlants; KQK10601; KQK10601; BRADI_2g55110v3.
DR GeneID; 100827766; -.
DR Gramene; KQK10601; KQK10601; BRADI_2g55110v3.
DR KEGG; bdi:100827766; -.
DR eggNOG; KOG1638; Eukaryota.
DR HOGENOM; CLU_065395_1_0_1; -.
DR InParanoid; I1HTF7; -.
DR OMA; SYGKHTE; -.
DR OrthoDB; 1574389at2759; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000008810; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0050213; F:progesterone 5-alpha-reductase activity; IEA:EnsemblPlants.
DR GO; GO:0009917; F:sterol 5-alpha reductase activity; IEA:EnsemblPlants.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IEA:EnsemblPlants.
DR InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
DR InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR InterPro; IPR039357; SRD5A/TECR.
DR PANTHER; PTHR10556; PTHR10556; 1.
DR Pfam; PF02544; Steroid_dh; 1.
DR PIRSF; PIRSF015596; 5_alpha-SR2; 1.
DR PROSITE; PS50244; S5A_REDUCTASE; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid biosynthesis; Lipid biosynthesis; Lipid metabolism;
KW Membrane; Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..271
FT /note="Protein DEETIOLATED 2"
FT /id="PRO_0000455303"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 271 AA; 29768 MW; C01217E9DE63C11F CRC64;
MSAAAGGDAL FSRCLLTLFL LSPITVFLLR FVSAPYGRLS RPGWGPAIPA AVAWCLMESP
TLWLPPLLLL ASSPFSRSPL LTAATTSPLA ALPPALYAVH YVHRTLVHPL RLLRLRRAPA
PVPLLVAACA FAFNLLNAYV QARSWALHAA HPASASLFAL ARCLVGLALF AWGMRVNIAS
DKELLRLKEA GGGYKIPRGG WFDAVTCPNY FGEVVEWLGY SLVAWTPAAW AFFLYTCANL
GPRARDHRQW YLQKFGREYP ASRTALVPYI Y
