DET2_GOSHI
ID DET2_GOSHI Reviewed; 258 AA.
AC Q2QDF6; Q8H1T2;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=Steroid 5-alpha-reductase DET2 {ECO:0000303|PubMed:17565582};
DE Short=GhDET2 {ECO:0000303|PubMed:17565582};
DE EC=1.3.1.22 {ECO:0000269|PubMed:17565582};
GN Name=DET2 {ECO:0000303|PubMed:16461577, ECO:0000303|PubMed:17565582};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Xuzhou142;
RX PubMed=16461577; DOI=10.1105/tpc.105.040303;
RA Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J.,
RA Wei L.-P., Wang Z.-Y., Zhu Y.-X.;
RT "Transcriptome profiling, molecular biological, and physiological studies
RT reveal a major role for ethylene in cotton fiber cell elongation.";
RL Plant Cell 18:651-664(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC
RP ACTIVITY, TISSUE SPECIFICITY, AND PATHWAY.
RC STRAIN=cv. Xuzhou142; TISSUE=Fiber;
RX PubMed=17565582; DOI=10.1111/j.1365-313x.2007.03144.x;
RA Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y.,
RA Pei Y.;
RT "GhDET2, a steroid 5alpha-reductase, plays an important role in cotton
RT fiber cell initiation and elongation.";
RL Plant J. 51:419-430(2007).
CC -!- FUNCTION: Involved in a reduction step in the biosynthesis of the plant
CC steroid, brassinolide (BL) (PubMed:17565582). Promotes cotton fibers
CC (seed trichomes) initiation and elongation (PubMed:17565582).
CC {ECO:0000269|PubMed:17565582}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid +
CC H(+) + NADPH; Xref=Rhea:RHEA:54384, ChEBI:CHEBI:13601,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47909, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000269|PubMed:17565582};
CC -!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
CC {ECO:0000269|PubMed:17565582}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Accumulates in fibers (seed trichomes) during both
CC their initiation and elongation phases. Also present in roots,
CC hypocotyls, leaves, flowers and ovules, and barely in cotyledons.
CC {ECO:0000269|PubMed:16461577, ECO:0000269|PubMed:17565582}.
CC -!- DISRUPTION PHENOTYPE: Impaired fiber initiation and elongation.
CC {ECO:0000269|PubMed:17565582}.
CC -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
CC {ECO:0000305}.
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DR EMBL; DQ116446; AAZ83346.1; -; mRNA.
DR EMBL; AY141136; AAN28012.1; -; mRNA.
DR RefSeq; NP_001314480.1; NM_001327551.1.
DR AlphaFoldDB; Q2QDF6; -.
DR SMR; Q2QDF6; -.
DR STRING; 3635.Q2QDF6; -.
DR GeneID; 107948867; -.
DR KEGG; ghi:107948867; -.
DR OMA; WLEWLGF; -.
DR UniPathway; UPA00381; -.
DR Proteomes; UP000189702; Genome assembly.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IEA:InterPro.
DR GO; GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0050213; F:progesterone 5-alpha-reductase activity; IDA:UniProtKB.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR GO; GO:0090378; P:seed trichome elongation; IMP:UniProtKB.
DR GO; GO:0090377; P:seed trichome initiation; IMP:UniProtKB.
DR InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
DR InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR InterPro; IPR039357; SRD5A/TECR.
DR PANTHER; PTHR10556; PTHR10556; 1.
DR Pfam; PF02544; Steroid_dh; 1.
DR PIRSF; PIRSF015596; 5_alpha-SR2; 1.
DR PROSITE; PS50244; S5A_REDUCTASE; 1.
PE 1: Evidence at protein level;
KW Membrane; NADP; Oxidoreductase; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..258
FT /note="Steroid 5-alpha-reductase DET2"
FT /id="PRO_0000418516"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 258 AA; 30421 MW; 17489CA9E27964B2 CRC64;
MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA WFLMESPTLW
LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA RNTTQTRGFP VSVAFMAFGF
NLLNGYLQAR WVSHYKDDYE NEELFWWRFL AGLLIFVVGM WVNVRADKVL VGLKKQGDGG
YKIPRGGLFE LVSCPNYFGE IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE
KFKDDYPKDR KAVIPFIY
