DEUP1_HUMAN
ID DEUP1_HUMAN Reviewed; 604 AA.
AC Q05D60; E9PJR5; Q8NEF1; Q96LL7;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Deuterosome assembly protein 1 {ECO:0000312|HGNC:HGNC:26344};
DE AltName: Full=Coiled-coil domain-containing protein 67;
GN Name=DEUP1 {ECO:0000312|HGNC:HGNC:26344}; Synonyms=CCDC67;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lung, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-604 (ISOFORM 1), AND VARIANT
RP LYS-504.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
CC -!- FUNCTION: Key structural component of the deuterosome, a structure that
CC promotes de novo centriole amplification in multiciliated cells.
CC Deuterosome-mediated centriole amplification occurs in terminally
CC differentiated multiciliated cells and can generate more than 100
CC centrioles. Probably sufficient for the specification and formation of
CC the deuterosome inner core. Interacts with CEP152 and recruits PLK4 to
CC activate centriole biogenesis (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CEP152; the interaction is mutually exclusive
CC with CEP63. {ECO:0000250}.
CC -!- INTERACTION:
CC Q05D60; P49418: AMPH; NbExp=3; IntAct=EBI-748597, EBI-7121510;
CC Q05D60; Q0P5N6: ARL16; NbExp=3; IntAct=EBI-748597, EBI-10186132;
CC Q05D60; Q5PSV4: BRMS1L; NbExp=3; IntAct=EBI-748597, EBI-5666615;
CC Q05D60; Q8TD31-3: CCHCR1; NbExp=3; IntAct=EBI-748597, EBI-10175300;
CC Q05D60; Q86XR8-3: CEP57; NbExp=3; IntAct=EBI-748597, EBI-11752486;
CC Q05D60; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-748597, EBI-742054;
CC Q05D60; Q05D60: DEUP1; NbExp=8; IntAct=EBI-748597, EBI-748597;
CC Q05D60; Q9BY27: DGCR6L; NbExp=9; IntAct=EBI-748597, EBI-742953;
CC Q05D60; O60941-5: DTNB; NbExp=3; IntAct=EBI-748597, EBI-11984733;
CC Q05D60; P62508: ESRRG; NbExp=3; IntAct=EBI-748597, EBI-2834260;
CC Q05D60; P62508-3: ESRRG; NbExp=3; IntAct=EBI-748597, EBI-12001340;
CC Q05D60; O00471: EXOC5; NbExp=11; IntAct=EBI-748597, EBI-949824;
CC Q05D60; Q9Y247: FAM50B; NbExp=3; IntAct=EBI-748597, EBI-742802;
CC Q05D60; Q8TES7-6: FBF1; NbExp=6; IntAct=EBI-748597, EBI-10244131;
CC Q05D60; P55040: GEM; NbExp=6; IntAct=EBI-748597, EBI-744104;
CC Q05D60; Q08379: GOLGA2; NbExp=6; IntAct=EBI-748597, EBI-618309;
CC Q05D60; Q9BT25: HAUS8; NbExp=3; IntAct=EBI-748597, EBI-2558143;
CC Q05D60; P42858: HTT; NbExp=15; IntAct=EBI-748597, EBI-466029;
CC Q05D60; O14879: IFIT3; NbExp=4; IntAct=EBI-748597, EBI-745127;
CC Q05D60; Q8IY31: IFT20; NbExp=3; IntAct=EBI-748597, EBI-744203;
CC Q05D60; Q8WYH8: ING5; NbExp=3; IntAct=EBI-748597, EBI-488533;
CC Q05D60; Q7L273: KCTD9; NbExp=3; IntAct=EBI-748597, EBI-4397613;
CC Q05D60; Q9BVG8: KIFC3; NbExp=3; IntAct=EBI-748597, EBI-2125614;
CC Q05D60; Q9BVG8-5: KIFC3; NbExp=5; IntAct=EBI-748597, EBI-14069005;
CC Q05D60; P19012: KRT15; NbExp=6; IntAct=EBI-748597, EBI-739566;
CC Q05D60; Q7Z3Y7: KRT28; NbExp=3; IntAct=EBI-748597, EBI-11980489;
CC Q05D60; P12035: KRT3; NbExp=3; IntAct=EBI-748597, EBI-2430095;
CC Q05D60; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-748597, EBI-739832;
CC Q05D60; Q8IVT4: MGC50722; NbExp=3; IntAct=EBI-748597, EBI-14086479;
CC Q05D60; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-748597, EBI-741158;
CC Q05D60; O75928-2: PIAS2; NbExp=3; IntAct=EBI-748597, EBI-348567;
CC Q05D60; Q96KQ4: PPP1R13B; NbExp=3; IntAct=EBI-748597, EBI-1105153;
CC Q05D60; Q06455-2: RUNX1T1; NbExp=3; IntAct=EBI-748597, EBI-11984663;
CC Q05D60; P12757: SKIL; NbExp=3; IntAct=EBI-748597, EBI-2902468;
CC Q05D60; P63165: SUMO1; NbExp=3; IntAct=EBI-748597, EBI-80140;
CC Q05D60; Q9NU19: TBC1D22B; NbExp=4; IntAct=EBI-748597, EBI-8787464;
CC Q05D60; P0C1Z6-2: TFPT; NbExp=3; IntAct=EBI-748597, EBI-10178002;
CC Q05D60; Q9BYV2: TRIM54; NbExp=6; IntAct=EBI-748597, EBI-2130429;
CC Q05D60; P40222: TXLNA; NbExp=11; IntAct=EBI-748597, EBI-359793;
CC Q05D60; Q8N3L3: TXLNB; NbExp=11; IntAct=EBI-748597, EBI-6116822;
CC Q05D60; Q8N6Y0: USHBP1; NbExp=3; IntAct=EBI-748597, EBI-739895;
CC Q05D60; Q15911-2: ZFHX3; NbExp=3; IntAct=EBI-748597, EBI-10237226;
CC Q05D60; Q9UQR1: ZNF148; NbExp=3; IntAct=EBI-748597, EBI-2688184;
CC Q05D60; Q14592: ZNF460; NbExp=3; IntAct=EBI-748597, EBI-2555738;
CC Q05D60; Q7Z3I7: ZNF572; NbExp=9; IntAct=EBI-748597, EBI-10172590;
CC Q05D60; Q8N720: ZNF655; NbExp=3; IntAct=EBI-748597, EBI-625509;
CC Q05D60; Q9Y2P0: ZNF835; NbExp=3; IntAct=EBI-748597, EBI-5667516;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Localizes to the
CC deuterosome. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q05D60-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q05D60-2; Sequence=VSP_027367, VSP_027368;
CC -!- MISCELLANEOUS: CEP63 and DEUP1 paralogs are both involved in centriole
CC amplification: while CEP63 mediates mother-centriole-dependent
CC centriole duplication, DEUP1 mediates de novo centriole amplification
CC in multiciliated cells.
CC -!- SIMILARITY: Belongs to the CEP63 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP003969; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP004242; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017929; AAH17929.1; ALT_SEQ; mRNA.
DR EMBL; BC031247; AAH31247.2; -; mRNA.
DR EMBL; AK058122; BAB71673.1; ALT_SEQ; mRNA.
DR CCDS; CCDS44707.1; -. [Q05D60-1]
DR RefSeq; NP_857596.2; NM_181645.3. [Q05D60-1]
DR RefSeq; XP_005273859.1; XM_005273802.1. [Q05D60-1]
DR AlphaFoldDB; Q05D60; -.
DR SMR; Q05D60; -.
DR BioGRID; 127746; 77.
DR IntAct; Q05D60; 59.
DR MINT; Q05D60; -.
DR STRING; 9606.ENSP00000298050; -.
DR iPTMnet; Q05D60; -.
DR PhosphoSitePlus; Q05D60; -.
DR BioMuta; DEUP1; -.
DR DMDM; 156630476; -.
DR EPD; Q05D60; -.
DR MassIVE; Q05D60; -.
DR MaxQB; Q05D60; -.
DR PaxDb; Q05D60; -.
DR PeptideAtlas; Q05D60; -.
DR PRIDE; Q05D60; -.
DR ProteomicsDB; 21226; -.
DR ProteomicsDB; 58400; -. [Q05D60-1]
DR ProteomicsDB; 58401; -. [Q05D60-2]
DR Antibodypedia; 2572; 95 antibodies from 19 providers.
DR DNASU; 159989; -.
DR Ensembl; ENST00000298050.9; ENSP00000298050.3; ENSG00000165325.15. [Q05D60-1]
DR GeneID; 159989; -.
DR KEGG; hsa:159989; -.
DR MANE-Select; ENST00000298050.9; ENSP00000298050.3; NM_181645.4; NP_857596.2.
DR UCSC; uc001pdq.5; human. [Q05D60-1]
DR UCSC; uc058gma.1; human.
DR CTD; 159989; -.
DR DisGeNET; 159989; -.
DR GeneCards; DEUP1; -.
DR HGNC; HGNC:26344; DEUP1.
DR HPA; ENSG00000165325; Group enriched (fallopian tube, testis).
DR neXtProt; NX_Q05D60; -.
DR OpenTargets; ENSG00000165325; -.
DR PharmGKB; PA143485419; -.
DR VEuPathDB; HostDB:ENSG00000165325; -.
DR eggNOG; ENOG502QRBJ; Eukaryota.
DR GeneTree; ENSGT00940000153190; -.
DR InParanoid; Q05D60; -.
DR OMA; ARTSPCE; -.
DR OrthoDB; 943593at2759; -.
DR PhylomeDB; Q05D60; -.
DR TreeFam; TF330595; -.
DR PathwayCommons; Q05D60; -.
DR SignaLink; Q05D60; -.
DR BioGRID-ORCS; 159989; 9 hits in 1041 CRISPR screens.
DR ChiTaRS; DEUP1; human.
DR GenomeRNAi; 159989; -.
DR Pharos; Q05D60; Tbio.
DR PRO; PR:Q05D60; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q05D60; protein.
DR Bgee; ENSG00000165325; Expressed in sperm and 92 other tissues.
DR ExpressionAtlas; Q05D60; baseline and differential.
DR Genevisible; Q05D60; HS.
DR GO; GO:0005814; C:centriole; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0098536; C:deuterosome; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0007099; P:centriole replication; IBA:GO_Central.
DR GO; GO:0098535; P:de novo centriole assembly involved in multi-ciliated epithelial cell differentiation; ISS:UniProtKB.
DR GO; GO:1903251; P:multi-ciliated epithelial cell differentiation; ISS:UniProtKB.
DR InterPro; IPR031470; Cep63/Deup1_N.
DR InterPro; IPR029611; Deup1.
DR PANTHER; PTHR18875:SF5; PTHR18875:SF5; 1.
DR Pfam; PF17045; CEP63; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..604
FT /note="Deuterosome assembly protein 1"
FT /id="PRO_0000297829"
FT COILED 14..59
FT /evidence="ECO:0000255"
FT COILED 85..197
FT /evidence="ECO:0000255"
FT COILED 226..278
FT /evidence="ECO:0000255"
FT COILED 336..399
FT /evidence="ECO:0000255"
FT COILED 558..601
FT /evidence="ECO:0000255"
FT MOD_RES 547
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5U3Z6"
FT VAR_SEQ 1..262
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027367"
FT VAR_SEQ 263
FT /note="Q -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027368"
FT VARIANT 175
FT /note="S -> F (in dbSNP:rs34205920)"
FT /id="VAR_050759"
FT VARIANT 440
FT /note="E -> G (in dbSNP:rs12282288)"
FT /id="VAR_059601"
FT VARIANT 440
FT /note="E -> Q (in dbSNP:rs12288277)"
FT /id="VAR_050760"
FT VARIANT 504
FT /note="Q -> K (in dbSNP:rs2259633)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_050761"
FT CONFLICT 440
FT /note="E -> R (in Ref. 3; BAB71673)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 604 AA; 70977 MW; 0BC0B1C207D2EA2A CRC64;
MENQAHNTMG TSPCEAELQE LMEQIDIMVS NKKMDWERKM RALETRLDLR DQELANAQTC
LDQKGQEVGL LRQKLDSLEK CNLAMTQNYE GQLQSLKAQF SKLTNNFEKL RLHQMKQNKV
PRKELPHLKE EIPFELSNLN QKLEEFRAKS REWDKQEILY QTHLISLDAQ QKLLSEKCNQ
FQKQAQSYQT QLNGKKQCLE DSSSEIPRLI CDPDPNCEIN ERDEFIIEKL KSAVNEIALS
RNKLQDENQK LLQELKMYQR QCQAMEAGLS EVKSELQSRD DLLRIIEMER LQLHRELLKI
GECQNAQGNK TRLESSYLPS IKEPERKIKE LFSVMQDQPN HEKELNKIRS QLQQVEEYHN
SEQERMRNEI SDLTEELHQK EITIATVTKK AALLEKQLKM ELEIKEKMLA KQKVSDMKYK
AVRTENTHLK GMMGDLDPGE YMSMDFTNRE QSRHTSINKL QYENERLRND LAKLHVNGKS
TWTNQNTYEE TGRYAYQSQI KVEQNEERLS HDCEPNRSTM PPLPPSTFQA KEMTSPLVSD
DDVFPLSPPD MSFPASLAAQ HFLLEEEKRA KELEKLLNTH IDELQRHTEF TLNKYSKLKQ
NRHI
