ID   DEUP1_MOUSE             Reviewed;         601 AA.
AC   Q7M6Y5; E9QL16; Q4VA46;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 3.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Deuterosome assembly protein 1 {ECO:0000250|UniProtKB:Q05D60};
DE   AltName: Full=Coiled-coil domain-containing protein 67;
GN   Name=Deup1 {ECO:0000250|UniProtKB:Q05D60}; Synonyms=Ccdc67;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 388-394, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=OF1; TISSUE=Hippocampus;
RA   Lubec G., Sunyer B., Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [4]
RP   IDENTIFICATION OF 11-601.
RC   STRAIN=C57BL/6J;
RX   PubMed=12783626; DOI=10.1186/1471-2164-4-22;
RA   Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.;
RT   "Gene discovery in the hamster: a comparative genomics approach for gene
RT   annotation by sequencing of hamster testis cDNAs.";
RL   BMC Genomics 4:22-22(2003).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CEP152, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=24240477; DOI=10.1038/ncb2880;
RA   Zhao H., Zhu L., Zhu Y., Cao J., Li S., Huang Q., Xu T., Huang X., Yan X.,
RA   Zhu X.;
RT   "The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for
RT   vertebrate multiciliogenesis.";
RL   Nat. Cell Biol. 15:1434-1444(2013).
CC   -!- FUNCTION: Key structural component of the deuterosome, a structure that
CC       promotes de novo centriole amplification in multiciliated cells.
CC       Deuterosome-mediated centriole amplification occurs in terminally
CC       differentiated multiciliated cells and can generate more than 100
CC       centrioles. Probably sufficient for the specification and formation of
CC       the deuterosome inner core. Interacts with CEP152 and recruits PLK4 to
CC       activate centriole biogenesis. {ECO:0000269|PubMed:24240477}.
CC   -!- SUBUNIT: Interacts with CEP152; the interaction is mutually exclusive
CC       with CEP63. {ECO:0000269|PubMed:24240477}.
CC   -!- INTERACTION:
CC       Q7M6Y5; A2AUM9: Cep152; NbExp=2; IntAct=EBI-16081624, EBI-2554268;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24240477}.
CC       Note=Localizes to the deuterosome.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7M6Y5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7M6Y5-2; Sequence=VSP_041617;
CC   -!- TISSUE SPECIFICITY: Highly enriched in multicilia-abundant tissues
CC       (trachea and oviduct). {ECO:0000269|PubMed:24240477}.
CC   -!- MISCELLANEOUS: CEP63 and DEUP1 paralogs are both involved in centriole
CC       amplification: while CEP63 mediates mother-centriole-dependent
CC       centriole duplication, DEUP1 mediates de novo centriole amplification
CC       in multiciliated cells. {ECO:0000305|PubMed:24240477}.
CC   -!- SIMILARITY: Belongs to the CEP63 family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-22 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH96547.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=DAA01466.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC155249; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC096547; AAH96547.1; ALT_INIT; mRNA.
DR   EMBL; BK001332; DAA01466.1; ALT_INIT; mRNA.
DR   CCDS; CCDS22839.2; -. [Q7M6Y5-2]
DR   CCDS; CCDS90502.1; -. [Q7M6Y5-1]
DR   RefSeq; NP_861537.2; NM_181816.2. [Q7M6Y5-2]
DR   RefSeq; XP_006510222.1; XM_006510159.2. [Q7M6Y5-1]
DR   RefSeq; XP_006510223.1; XM_006510160.1.
DR   AlphaFoldDB; Q7M6Y5; -.
DR   SMR; Q7M6Y5; -.
DR   DIP; DIP-61755N; -.
DR   IntAct; Q7M6Y5; 2.
DR   STRING; 10090.ENSMUSP00000039912; -.
DR   iPTMnet; Q7M6Y5; -.
DR   PhosphoSitePlus; Q7M6Y5; -.
DR   PaxDb; Q7M6Y5; -.
DR   PRIDE; Q7M6Y5; -.
DR   ProteomicsDB; 279404; -. [Q7M6Y5-1]
DR   ProteomicsDB; 279405; -. [Q7M6Y5-2]
DR   Antibodypedia; 2572; 95 antibodies from 19 providers.
DR   DNASU; 234964; -.
DR   Ensembl; ENSMUST00000045513; ENSMUSP00000039912; ENSMUSG00000039977. [Q7M6Y5-1]
DR   Ensembl; ENSMUST00000115592; ENSMUSP00000111255; ENSMUSG00000039977. [Q7M6Y5-2]
DR   Ensembl; ENSMUST00000115593; ENSMUSP00000111256; ENSMUSG00000039977. [Q7M6Y5-2]
DR   GeneID; 234964; -.
DR   KEGG; mmu:234964; -.
DR   UCSC; uc009ogd.1; mouse. [Q7M6Y5-2]
DR   UCSC; uc009oge.1; mouse. [Q7M6Y5-1]
DR   CTD; 159989; -.
DR   MGI; MGI:2443026; Deup1.
DR   VEuPathDB; HostDB:ENSMUSG00000039977; -.
DR   eggNOG; ENOG502QRBJ; Eukaryota.
DR   GeneTree; ENSGT00940000153190; -.
DR   HOGENOM; CLU_027471_1_0_1; -.
DR   InParanoid; Q7M6Y5; -.
DR   OMA; ARTSPCE; -.
DR   OrthoDB; 943593at2759; -.
DR   PhylomeDB; Q7M6Y5; -.
DR   TreeFam; TF330595; -.
DR   BioGRID-ORCS; 234964; 1 hit in 58 CRISPR screens.
DR   ChiTaRS; Deup1; mouse.
DR   PRO; PR:Q7M6Y5; -.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; Q7M6Y5; protein.
DR   Bgee; ENSMUSG00000039977; Expressed in spermatid and 48 other tissues.
DR   ExpressionAtlas; Q7M6Y5; baseline and differential.
DR   Genevisible; Q7M6Y5; MM.
DR   GO; GO:0005814; C:centriole; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0098536; C:deuterosome; IDA:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   GO; GO:0007099; P:centriole replication; IBA:GO_Central.
DR   GO; GO:0098535; P:de novo centriole assembly involved in multi-ciliated epithelial cell differentiation; IDA:UniProtKB.
DR   GO; GO:1903251; P:multi-ciliated epithelial cell differentiation; IDA:UniProtKB.
DR   InterPro; IPR031470; Cep63/Deup1_N.
DR   InterPro; IPR029611; Deup1.
DR   PANTHER; PTHR18875:SF5; PTHR18875:SF5; 1.
DR   Pfam; PF17045; CEP63; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cilium biogenesis/degradation; Coiled coil;
KW   Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome.
FT   CHAIN           1..601
FT                   /note="Deuterosome assembly protein 1"
FT                   /id="PRO_0000297831"
FT   REGION          305..329
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          14..59
FT                   /evidence="ECO:0000255"
FT   COILED          86..196
FT                   /evidence="ECO:0000255"
FT   COILED          226..277
FT                   /evidence="ECO:0000255"
FT   COILED          354..397
FT                   /evidence="ECO:0000255"
FT   COILED          555..586
FT                   /evidence="ECO:0000255"
FT   MOD_RES         544
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U3Z6"
FT   VAR_SEQ         347..440
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_041617"
SQ   SEQUENCE   601 AA;  69678 MW;  66D8F0BC32D07A39 CRC64;
     MENQAHTTAG ASPCEAELQE LMEQIDIMVS NKKLDWERKM RALETRLDLR DQELANAQTC
     LDQKGQEVGL LRQKLDSLEK CNLVMTQNYE GQLQTLKAQF SKLTSNFEKL RLHQMKQNQI
     HRKESSSKEE LPFELSSLNQ KLEEFRAKSR EWDKQEVLYQ THLVSLDAQQ KLLSEKCSQF
     QKQAQNYQTQ LNGKKQCAED SSSEIPRLVC ESDPGCEATQ RDEFIIEKLK SAVSEIALSR
     NKLQDENQKL LQELKMYQRQ CQAMEAGLSE VKSELQSRDD LLRIIEMERL HLHRELLRMG
     EVQTAQDNRK RVESSYSPSP KEAERKRKEL FPMVSDQPNH EKELSKMRSQ LYQEEGLCSE
     QERLRSEISE LTQELHQKEV TIATVMKKAA LLERQLKIEL EIKERMLAKQ QVSDRRYKAV
     RTENTHLKGM MGDLDPARYL AVDLSNKKHS QCTSINKLEY ENERLRSDLA KLHGNGKAAW
     PNQSSYGEAG AYVFQSQLKT ETSGDRISQD CELNRSPTPL SPLPFQTKEM ASPLVGDNEV
     LALSPPDISF RASLAAQHFL MEEERRAKEL EKLLNTHIDE LQRHTEFTLN KYTKLKQSRH
     I