ID   DEVS_MYXXD              Reviewed;         214 AA.
AC   Q07766; Q1CW49;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=CRISPR-associated protein Cas5;
DE   AltName: Full=Fruiting body developmental protein S;
GN   Name=devS; Synonyms=cas5; OrderedLocusNames=MXAN_7261;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DK1622;
RX   PubMed=7693658; DOI=10.1128/jb.175.22.7450-7462.1993;
RA   Thoeny-Meyer L., Kaiser D.;
RT   "devRS, an autoregulated and essential genetic locus for fruiting body
RT   development in Myxococcus xanthus.";
RL   J. Bacteriol. 175:7450-7462(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN   [3]
RP   FUNCTION IN NEGATIVE AUTOREGULATION, INDUCTION, OPERON STRUCTURE, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=DK1622;
RX   PubMed=17369305; DOI=10.1128/jb.00187-07;
RA   Viswanathan P., Murphy K., Julien B., Garza A.G., Kroos L.;
RT   "Regulation of dev, an operon that includes genes essential for Myxococcus
RT   xanthus development and CRISPR-associated genes and repeats.";
RL   J. Bacteriol. 189:3738-3750(2007).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- FUNCTION: Has a role in fruiting body development, sporulation and
CC       aggregation. {ECO:0000269|PubMed:17369305}.
CC   -!- DEVELOPMENTAL STAGE: Operon expression begins by 6 hours after
CC       starvation has initiated development and is under strong negative
CC       autoregulation.
CC   -!- INDUCTION: Part of an operon going from at least MXAN_7266 to MXAN_7259
CC       that includes a CRISPR operon with transcription continuing into the
CC       pre-crRNA locus. {ECO:0000269|PubMed:17369305,
CC       ECO:0000269|PubMed:7693658}.
CC   -!- DISRUPTION PHENOTYPE: Essential for sporulation, decreases sporulation
CC       100 to 1000-fold. Negative autoregulation by this gene product relies
CC       on DNA both upstream and downstream of the operon's promoter.
CC       {ECO:0000269|PubMed:17369305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas5 family.
CC       {ECO:0000305}.
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DR   EMBL; L19029; AAA16135.1; -; Unassigned_DNA.
DR   EMBL; CP000113; ABF89647.1; -; Genomic_DNA.
DR   PIR; C49941; C49941.
DR   RefSeq; WP_011557180.1; NC_008095.1.
DR   AlphaFoldDB; Q07766; -.
DR   STRING; 246197.MXAN_7261; -.
DR   EnsemblBacteria; ABF89647; ABF89647; MXAN_7261.
DR   GeneID; 41364427; -.
DR   KEGG; mxa:MXAN_7261; -.
DR   eggNOG; ENOG502Z8P6; Bacteria.
DR   HOGENOM; CLU_1325024_0_0_7; -.
DR   OMA; LPYWVDH; -.
DR   OrthoDB; 1400944at2; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR013415; Cas5_bac.
DR   InterPro; IPR013422; CRISPR-assoc_prot_Cas5_N.
DR   TIGRFAMs; TIGR02586; cas5_cmx5_devS; 1.
DR   TIGRFAMs; TIGR02593; CRISPR_cas5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Fruiting body; Reference proteome; Sporulation.
FT   CHAIN           1..214
FT                   /note="CRISPR-associated protein Cas5"
FT                   /id="PRO_0000079872"
SQ   SEQUENCE   214 AA;  23303 MW;  748EA24915A86675 CRC64;
     MIALELSVPV ACWRKGRARE LVETEVLPPP ATCYGALLSL VGEQDRERHR GCRVTAGVLN
     APVISTVLRT FWRSKNLKVA KGNDENAAPD QQQLVIDARL VVWCDSREEP DSGESLEDRV
     VRAMREPGSV TRAGGWSLGE STHLINDARL LPEGRPPAGC RAFLTASTGA LTLPVWVDHV
     GTRGTRYEVG RLEEVLAAPE VQRLPRIPLA EGAG