DEVT_MYXXD
ID DEVT_MYXXD Reviewed; 570 AA.
AC P38586; Q1CW47;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=CRISPR-associated protein Cas8a1/Csx13;
DE AltName: Full=CRISPR-associated protein Cas8a1/Csx13, Myxan subtype;
DE AltName: Full=Fruiting body developmental protein T;
GN Name=devT; Synonyms=cas8a1; OrderedLocusNames=MXAN_7263;
OS Myxococcus xanthus (strain DK1622).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=246197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DK1622;
RX PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 346-570.
RC STRAIN=DK1622;
RX PubMed=7693658; DOI=10.1128/jb.175.22.7450-7462.1993;
RA Thoeny-Meyer L., Kaiser D.;
RT "devRS, an autoregulated and essential genetic locus for fruiting body
RT development in Myxococcus xanthus.";
RL J. Bacteriol. 175:7450-7462(1993).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=DK1622;
RX PubMed=11872704; DOI=10.1128/jb.184.6.1540-1546.2002;
RA Boysen A., Ellehauge E., Julien B., Sogaard-Andersen L.;
RT "The DevT protein stimulates synthesis of FruA, a signal transduction
RT protein required for fruiting body morphogenesis in Myxococcus xanthus.";
RL J. Bacteriol. 184:1540-1546(2002).
RN [4]
RP INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=DK1622;
RX PubMed=17369305; DOI=10.1128/jb.00187-07;
RA Viswanathan P., Murphy K., Julien B., Garza A.G., Kroos L.;
RT "Regulation of dev, an operon that includes genes essential for Myxococcus
RT xanthus development and CRISPR-associated genes and repeats.";
RL J. Bacteriol. 189:3738-3750(2007).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC similarity). {ECO:0000250}.
CC -!- FUNCTION: Functions in an unknown fashion to stimulate transcription of
CC fruA independently of the intracellular A- and E-developmental signals.
CC -!- DEVELOPMENTAL STAGE: Operon expression begins by 6 hours after
CC starvation has initiated development and is under strong negative
CC regulation by DevS.
CC -!- INDUCTION: Part of an operon going from at least MXAN_7266 to MXAN_7259
CC that includes a CRISPR operon with transcription continuing into the
CC pre-crRNA locus. {ECO:0000269|PubMed:17369305}.
CC -!- DISRUPTION PHENOTYPE: Decreases sporulation 1000 to 10000-fold,
CC aggregation is delayed. Delays for C-signal-dependent methylation of
CC FrczCD and impairs fruA transcription. This mutant should not have
CC polar effects on downstream genes. {ECO:0000269|PubMed:11872704}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas8a1/Csx13
CC family. Myxan subtype subfamily. {ECO:0000305}.
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DR EMBL; CP000113; ABF87502.1; -; Genomic_DNA.
DR EMBL; L19029; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR PIR; A49941; A49941.
DR AlphaFoldDB; P38586; -.
DR STRING; 246197.MXAN_7263; -.
DR EnsemblBacteria; ABF87502; ABF87502; MXAN_7263.
DR KEGG; mxa:MXAN_7263; -.
DR eggNOG; ENOG502ZADP; Bacteria.
DR HOGENOM; CLU_531807_0_0_7; -.
DR OMA; QHNKMRP; -.
DR Proteomes; UP000002402; Chromosome.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR CDD; cd09713; Cas8c_I-C; 1.
DR InterPro; IPR027811; CRISPR-assoc_Csx13_C.
DR InterPro; IPR019989; CRISPR-assoc_Csx13_N.
DR TIGRFAMs; TIGR03486; cas_csx13_C; 1.
DR TIGRFAMs; TIGR03485; cas_csx13_N; 1.
PE 2: Evidence at transcript level;
KW Antiviral defense; Fruiting body; Reference proteome; Sporulation.
FT CHAIN 1..570
FT /note="CRISPR-associated protein Cas8a1/Csx13"
FT /id="PRO_0000066192"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 551..570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 376..377
FT /note="EA -> DR (in Ref. 2; L19029)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 61287 MW; B6DD202432EBA706 CRC64;
MACMAPRGPA AIPHPSSERA GLRRPARCAM AKAVNPRKKA LPAPLSIRLY APGMTPLLRA
GAGGLAASLR AILGSASPAA PWPSPVRLGP GTATVEQEAI HLDWGGKAPE ATLRALFGAS
FRVKQGFIDL PGTRPPGAPE PPPELAAALH DALKVTFLQH GKSTQGGARR RVTFEVDARP
VIVESQGYDS FVHQTAWQSV LEALEVGSTS LASWAYPGAA ERHIGVRVTK VEYTAAEALC
ACFALVGCVS YKLPQLRGGA FVALAPTNLV RFAELRPGLT PKRLRDVAVA GASDAVLAAQ
LVMAQEAGKK RLGAVLGTTE AVALRQMPWN AQQKIRGAVV RQDAVLEEVL DRYEAAAAAL
PHTLRVRKPE GKATGEASYF IAISALRAFI TENLAASRPW YADFATATTA EGRFIHDYRD
RDNLGALLWH ERKGLIAMHP YLGEAEQWLV QSVHLALRSR FKSIYADTKE SAPATRSNRL
KGERERLRLS FAGAKTPEQV RAALADLWSR AGTNRELQEH WRDILQLLGP ERWRAARDLA
LVALASYQGK GGEAAELEDA DEAAGASEQS
