DEXH1_ARATH
ID DEXH1_ARATH Reviewed; 995 AA.
AC F4ILR7; Q56WR8; Q9SJ58;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH1 {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
GN OrderedLocusNames=At2g35920 {ECO:0000312|Araport:AT2G35920};
GN ORFNames=F11F19.17 {ECO:0000312|EMBL:AAD21465.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 415-995.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21465.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AY070430; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC007017; AAD21465.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09177.1; -; Genomic_DNA.
DR EMBL; AY070430; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK221966; BAD94478.1; -; mRNA.
DR PIR; F84774; F84774.
DR RefSeq; NP_850255.1; NM_179924.2.
DR AlphaFoldDB; F4ILR7; -.
DR SMR; F4ILR7; -.
DR STRING; 3702.AT2G35920.1; -.
DR iPTMnet; F4ILR7; -.
DR PaxDb; F4ILR7; -.
DR PRIDE; F4ILR7; -.
DR ProteomicsDB; 224249; -.
DR EnsemblPlants; AT2G35920.1; AT2G35920.1; AT2G35920.
DR GeneID; 818165; -.
DR Gramene; AT2G35920.1; AT2G35920.1; AT2G35920.
DR KEGG; ath:AT2G35920; -.
DR Araport; AT2G35920; -.
DR TAIR; locus:2039280; AT2G35920.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_001832_1_0_1; -.
DR InParanoid; F4ILR7; -.
DR PRO; PR:F4ILR7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4ILR7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..995
FT /note="DExH-box ATP-dependent RNA helicase DExH1"
FT /id="PRO_0000435291"
FT DOMAIN 238..405
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 484..663
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 156..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 352..355
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT COMPBIAS 156..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 251..258
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 571
FT /note="E -> K (in Ref. 4; BAD94478)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 995 AA; 111039 MW; F31AB681EAD155A2 CRC64;
MPPHGPNSQG GRRGGGHSSG RRGGRGGGGR GGGGGGRGEQ RWWDPVWRAE RLRQQQAEME
VLDENEWWNK IEQWKTGGEQ EMLIKRNFSR GDQQTLSDMA LQMGLYFHAY NKGKALVVSK
VPLPDYRADL DERHGSTQKE IKMSTETERK LGSLLKTTQE SGSSGASASA FNDQQDRTST
LGLKRPDSAS KLPDSLEKEK FSFALKERQE KLKATESVKA LKAFREKLPA FKMKEEFLNS
VSQNQVLVVS GETGCGKTTQ LPQFILEEEI SSLRGADCNI ICTQPRRISA ISVASRISAE
RGESIGESVG YQIRLESKRS DQTRLLFCTT GVLLRRLIED PNLTNVSHLL VDEIHERGMN
EDFLLIILRD LLPRRPDLRL ILMSATINAD MFSTYFGNSP TMHIPGFTFP VAELFLEDVL
EKSRYNIKSS DSGNYQGSSR GRRRESESKK DDLTTLFEDI DINSHYKSYS SATRNSLEAW
SGAQIDVDLV EATIEHICRL EGGGAILVFL TGWDEISKLL EKINMNNFLG DSSKFLVLPL
HGSMPTVNQR EIFDRPPPNK RKIVLATNIA ESSITIDDVV YVVDCGKAKE TSYDALNKVA
CLLPSWISKA SAHQRRGRAG RVQAGVCYRL YPKVIYDAFP QYQLPEIIRT PLQELCLHIK
SLQVGSIGSF LAKALQPPDA LAVENAIELL KTIGALNDVE ELTPLGRHLC TLPVDPNIGK
MLLIGAIFQC VNPALTIAAA LAYRSPFVLP LNRKEEADEA KRYFAGDSCS DHIALLKAYE
GYRDAKRGGN EKDFCWQNFL SPVTLRMMED MRNQFLDLLS DIGFVDKSKP NAYNQYSYDM
EMISAVLCAG LYPNVVQCKR RGKRTAFYTK ELGKVDIHPG SVNARVNLFS LPYLVYSEKV
KTTSVYIRDS TNISDYALLM FGGNLIPSKT GEGIEMLGGY LHFSASKNIL ELIQRLRGEV
DKLLNKKIED PSLDITVEGK GVVSAVVELL RSQKH
