DEXH5_ARATH
ID DEXH5_ARATH Reviewed; 1113 AA.
AC F4IM84; Q8S8F6; Q9ZU53;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH5, mitochondrial {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=At2g01130 {ECO:0000312|Araport:AT2G01130};
GN ORFNames=F10A8.1 {ECO:0000312|EMBL:AAD14515.3},
GN F23H14 {ECO:0000312|EMBL:AAM15307.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD14515.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006200; AAD14515.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006837; AAM15307.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05405.1; -; Genomic_DNA.
DR PIR; A84421; A84421.
DR RefSeq; NP_178223.2; NM_126175.4.
DR AlphaFoldDB; F4IM84; -.
DR SMR; F4IM84; -.
DR STRING; 3702.AT2G01130.1; -.
DR iPTMnet; F4IM84; -.
DR PaxDb; F4IM84; -.
DR PRIDE; F4IM84; -.
DR ProteomicsDB; 224131; -.
DR EnsemblPlants; AT2G01130.1; AT2G01130.1; AT2G01130.
DR GeneID; 814642; -.
DR Gramene; AT2G01130.1; AT2G01130.1; AT2G01130.
DR KEGG; ath:AT2G01130; -.
DR Araport; AT2G01130; -.
DR TAIR; locus:2038796; AT2G01130.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_001832_1_0_1; -.
DR InParanoid; F4IM84; -.
DR OrthoDB; 278674at2759; -.
DR PRO; PR:F4IM84; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IM84; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding;
KW Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 34..1113
FT /note="DExH-box ATP-dependent RNA helicase DExH5,
FT mitochondrial"
FT /id="PRO_0000435295"
FT DOMAIN 230..397
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 484..658
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 344..347
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT BINDING 243..250
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1113 AA; 125749 MW; 86B23A1FDDDB18C1 CRC64;
MTNRLPPSLY VPPHQRLGSD YGFNPVPLSP VRYVSAYDDR VSEDRQPQEG TFHCADLDDW
NKRFSMLLKD SLKQEVISRE KKDRRDFDKL AALATTLGLY SHAYAKVVVF SKIPLPNYRF
DLDDKKPQRE VNLHTDLLQR VEAYLTEYLS KSSNRIDRVP ANSVSRTSSI SSTDEWFSEQ
PLPISATKIL WQRSLQLRDR QQYWQASVEG QRMLDSRTSL PAFKQRHSVL TAISQNQVIV
ISGETGCGKT TQIPQFILES EIEANRGAFS SIICTQPRRI SAMSVSERVA YERGEQLGES
VGYKVRLEGV KGRDTRLLFC TTGILLRRLL VDRNLRGVTH VIVDEIHERG MNEDFLLIIL
KDLLSRRSEL KLILMSATLD AELFSSYFGG AGVIYIPGFT YPVRSHFLED ILEMTRYRLT
PYNQIDDYGQ ERTWKMNKQI PKKRKSQITF VVEDALRAAD FKEFSPETRE SLSCWYPDCI
GFNLIEFLLC NICENEGPGG ILIFLTGWDD ISSLKEKLQI HPIFGNPDLV MLLACHGSME
TFEQRLIFEE PASGVRKIVL ATNIAETSIT INDVAFVIDC GKAKETSYDA LNNTPCLLPS
WISKVSAQQR RGRAGRVRPG QCYHLYPKCV YDAFAEYQLP EILRTPLHSL CLQIKSLNLG
SISEFLSRAL QSPELLAVQK AIAFLKIIGA LDENEDLTTL GRYLSKLPME PKLGKMLILG
AILGCLDPIL TVAAGLSVRD PFLTPQDKKD LAEAAKSQFS RDHSDHLALV RAYEGWKKAE
EESAVYDYCW KNFLSIQSLR AIDSLRKEFF SLLKDTGLID GNPSICNSEG NDANLTRAVI
CYGMYPGICS VVHNERSFSL KTMEDGQVLL YSNSENARET KIPYPWLVFN EKIKVNSVFL
RDSTACSDST LILFGGSISK GDTDGHLKML GGYLEFFMKP DVAEIYQTLK KELDELIQNK
LLNPKVDMQA HRELLSAIRL LVSEDGCDGR FVFGHQILRP LEISALSTKP SLFSRTESGP
GGDNSKSQLQ TILTRAGYTV PMYKTKQLKN NKFQTTVEFN ETQIMGQPCS NKKSAEKDAA
AEAIQWLKGG AKESHEQVNH MSKLLKKGKK DHL
