DEXH7_ARATH
ID DEXH7_ARATH Reviewed; 1459 AA.
AC F4I9Q5; Q0WTQ8; Q84VZ2; Q9C6F9;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH7, chloroplastic {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=At1g58060 {ECO:0000312|Araport:AT1G58060};
GN ORFNames=T15M6.7 {ECO:0000312|EMBL:AAG50701.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1172-1459.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1200-1459.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50701.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC079604; AAG50701.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33493.1; -; Genomic_DNA.
DR EMBL; AK227490; BAE99490.1; -; mRNA.
DR EMBL; BT004601; AAO42847.1; -; mRNA.
DR PIR; G96613; G96613.
DR RefSeq; NP_176103.2; NM_104588.4.
DR AlphaFoldDB; F4I9Q5; -.
DR SMR; F4I9Q5; -.
DR STRING; 3702.AT1G58060.1; -.
DR PaxDb; F4I9Q5; -.
DR PRIDE; F4I9Q5; -.
DR ProteomicsDB; 224251; -.
DR EnsemblPlants; AT1G58060.1; AT1G58060.1; AT1G58060.
DR GeneID; 842173; -.
DR Gramene; AT1G58060.1; AT1G58060.1; AT1G58060.
DR KEGG; ath:AT1G58060; -.
DR Araport; AT1G58060; -.
DR TAIR; locus:2196265; AT1G58060.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_001832_1_4_1; -.
DR InParanoid; F4I9Q5; -.
DR OMA; HQQNEKK; -.
DR OrthoDB; 278674at2759; -.
DR PRO; PR:F4I9Q5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I9Q5; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Helicase; Hydrolase; Nucleotide-binding; Plastid;
KW Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..37
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 38..1459
FT /note="DExH-box ATP-dependent RNA helicase DExH7,
FT chloroplastic"
FT /id="PRO_0000435297"
FT DOMAIN 624..801
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 901..1076
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 742..745
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT COMPBIAS 9..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 637..644
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1459 AA; 163600 MW; CFC795CC532223C9 CRC64;
MAPKKKPQKQ SNKAASSSSS SKSNYQKPSS GPKLQISAEN EDRLRRLLLN SGRSGPSIPA
PISNSLSKAQ KTKKLNNVYE KLSCEGFVDD QIELALSSLR DGATFEAALD WLCLNLPSHE
LPVKFSTGAS RFPSTGGSVG VISTSRDDWN DSTDSSVRVE EEEPAVFVRV KGKQDEEDTL
SSDKSSQADW IRQYMMRQEE EELECWEDEV DGIDPRNKVS GPRPFDVIAK EYYSARSDAI
KAKEKRDKRG QEQAGLAIRK LKQEISDLGL SEAMLESEFQ REHAFESATE QESTCPISDN
LHESVDADDV SVQMLDNLTL NTNPAESYES EEIQTKALPS SSSGQDFVAS DEDSEDVELG
DTFFEEIPPS EISPHELLEL QKEEKMRELR SEKNLGKLDG IWKKGEAQKI PKAFLHQLCQ
RSGWEAPKFN KETGEGRNFS YTVSILRKAS GRGKNRQAGG LVTLQLPPKD ENFESIEDAQ
NKVAAFALHK LFSDLPVHFA ITEPYASLVL IWKQEELLCT TIQSTEEDRR ANFVDKLLEE
DSFSLTTSSS SFENSLPLVD SYVKDKDDLG VVKSNNRAKR DSYIEAECLS LQRKQENKKR
TQKYKDMLKT RTALPISEVK NGILQHLKEK DVLVVCGETG SGKTTQVPQF ILDDMIDSGH
GGYCNIICTQ PRRIAAISVA QRVADERCES SPGLDDSLVG YQVRLESARS DKTRLLFCTT
GILLRKLAGD RTLNDVTHII VDEVHERSLL GDFLLIILKS LIEKQSCDNT SRKLKVILMS
ATVDADLFSR YFGHCPVITA QGRTHPVTTH FLEEIYESIN YLLAPDSPAA LRSDTSIKDK
LGSVNDRRGK KNLVLAGWGD DYLLSEDCLN PFYVSSNYNS YSDQTQQNLK RLNEDRIDYE
LLEELICHID DTCEEGAILI FLPGVAEIYM LLDMLAASYR FRGPAADWLL PLHSSIASSE
QRKVFLRPPK GLRKVIAATN IAETSITIDD VVYVIDSGKH KENRYNPQKK LSSMVEDWIS
QANARQRTGR AGRVKPGICF SLYTRYRFEK LMRPYQVPEM LRMPLVELCL QIKLLGLGHI
KPFLSRALEP PSEGAMTSAI SLLHEVGAVE GDEELTPLGH HLAKLPVDVL IGKMLLYGGI
FGCLSPILSI AAFLSYKSPF IYPKDEKQNV DRVKLALLSD NGVSSSDLNN NDRQSDHLLM
MVAYDKWVKI LQERGMKAAQ RFCESKFLSS SVMRMIRDMR VQFGTLLADI GLINLPKTGE
FSGRKKENLD VWFSDPTQPF NMYSQQPEVV KAILCAGLYP NIAANDKGIT ETTFNSLTKQ
GNQTKSYSAW YDGRREVHIH PSSINSNFKA FQNPFLVFLE KVETNKVYLR DTTIVSPFSI
LLFGGSINVH HQSGSVTIDG WLKVAAPAQT AVLFKELRLT LHSILKDLIR KPEKSGIVHN
EVVKSMVHLL IEEGKPQHK
