DEXHE_ARATH
ID DEXHE_ARATH Reviewed; 2157 AA.
AC Q9FNQ1; Q8RY91;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH14 {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
DE AltName: Full=BRR2 homolog C {ECO:0000305};
DE Short=AtBRR2C {ECO:0000305};
DE AltName: Full=Pre-mRNA-splicing helicase BRR2C {ECO:0000305};
GN Name=BRR2C {ECO:0000305};
GN OrderedLocusNames=At5g61140 {ECO:0000312|Araport:AT5G61140};
GN ORFNames=MAF19.14 {ECO:0000312|EMBL:BAB10376.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1353-2157.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- FUNCTION: RNA helicase that plays an essential role in pre-mRNA
CC splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes.
CC Involved in spliceosome assembly, activation and disassembly.
CC {ECO:0000250|UniProtKB:P32639}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FNQ1-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
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DR EMBL; AB006696; BAB10376.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97426.1; -; Genomic_DNA.
DR EMBL; AY074505; AAL69489.2; -; mRNA.
DR RefSeq; NP_001190584.1; NM_001203655.2. [Q9FNQ1-1]
DR AlphaFoldDB; Q9FNQ1; -.
DR SMR; Q9FNQ1; -.
DR IntAct; Q9FNQ1; 1.
DR STRING; 3702.AT5G61140.2; -.
DR iPTMnet; Q9FNQ1; -.
DR PaxDb; Q9FNQ1; -.
DR PRIDE; Q9FNQ1; -.
DR ProteomicsDB; 224134; -. [Q9FNQ1-1]
DR EnsemblPlants; AT5G61140.2; AT5G61140.2; AT5G61140. [Q9FNQ1-1]
DR GeneID; 836235; -.
DR Gramene; AT5G61140.2; AT5G61140.2; AT5G61140. [Q9FNQ1-1]
DR KEGG; ath:AT5G61140; -.
DR Araport; AT5G61140; -.
DR TAIR; locus:2159451; AT5G61140.
DR eggNOG; KOG0952; Eukaryota.
DR HOGENOM; CLU_000335_2_1_1; -.
DR InParanoid; Q9FNQ1; -.
DR OMA; GTHHAGM; -.
DR OrthoDB; 154891at2759; -.
DR PhylomeDB; Q9FNQ1; -.
DR PRO; PR:Q9FNQ1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNQ1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; HDA:TAIR.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 2.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.300; -; 4.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 2.
DR Pfam; PF00271; Helicase_C; 2.
DR Pfam; PF02889; Sec63; 2.
DR SMART; SM00382; AAA; 2.
DR SMART; SM00487; DEXDc; 2.
DR SMART; SM00490; HELICc; 2.
DR SMART; SM00973; Sec63; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
DR SUPFAM; SSF52540; SSF52540; 4.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR PROSITE; PS51194; HELICASE_CTER; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Helicase; Hydrolase; mRNA processing;
KW mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome;
KW RNA-binding; Spliceosome.
FT CHAIN 1..2157
FT /note="DExH-box ATP-dependent RNA helicase DExH14"
FT /id="PRO_0000435301"
FT DOMAIN 517..699
FT /note="Helicase ATP-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 734..932
FT /note="Helicase C-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 1008..1315
FT /note="SEC63 1"
FT /evidence="ECO:0000255"
FT DOMAIN 1365..1540
FT /note="Helicase ATP-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 1571..1780
FT /note="Helicase C-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 1839..2150
FT /note="SEC63 2"
FT /evidence="ECO:0000255"
FT REGION 374..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 641..644
FT /note="DEVH box"
FT /evidence="ECO:0000305"
FT MOTIF 1482..1485
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT BINDING 530..537
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT BINDING 1378..1385
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 2157 AA; 244528 MW; B1E68E0663BF559F CRC64;
MVHNSPCNKH KTTSISWTRI LAGRCTGLFV QVSNRRRFRT QAIAFFLGEP TRGENTTEEK
NPKRHKYRDF VMLVQLPRLT SSLREPFDID QAYLRRKTIL QTLNKPRSSG NRLDESDLAK
RIVHQWEGAS LEVRQAYKQF IGAVVELIDR EVPSDEFREV AFSAYRLFNN PVEEDDSDIN
DNISISGKKL ELQNLVGHAV SDANVKNVAS FAQALYSIQP THQSETYADE VNGGAEFGAD
LVFNLPARFL VEASLDETGF VDVESNDAHT SFSEGWSGVS DTKNNLSAGK FNLSWLRDAC
GRMVRETNSQ LSREELAMAI CRFLDSDKPG EEIAGDLLDL VGDGAFETVQ DLIMHRKEIV
DAIHHGQMIL KSDKAASNTQ SRMPTYGTQV TVQTESAKQI EKLRRKEEKK NKRNADLGLE
SEISEANFSS LLEASEKKTA FEDLIGSGEA NSLALALPQG TVRKHLKGYE EVFIPPTPTA
QMKPGEKLIE IKELDDFAQA AFHGYKSLNR IQSRIFQTVY HTNENILVCA PTGAGKTNIA
MISVLHEIKQ HFRDGYLHKN EFKIVYVAPM KALAAEVTSA FSRRLAPLNM VVKELTGDMQ
LTKTELEETQ MIVTTPEKWD VITRKSSDMS MSMLVKLLII DEVHLLNDDR GAVIEALVAR
TLRQVESTQT MIRIVGLSAT LPSYLQVAQF LRVNTDTGLF YFDSSYRPVP LAQQYIGITE
HNFAARNELL NEICYKKVVD SIKQGHQAMI FVHSRKDTSK TAEKLVDLAR QYETLDLFTN
ETHPQFQLMK KDVMKSRNKD LVKFFEAGFG IHHAGMLRSD RTLTERLFSD GLLKVLVCTA
TLAWGVNLPA HTVVIKGTQL YDAKAGGWKD LGMLDVMQIF GRAGRPQFDK SGEGIIITSH
DKLAYYLRLL TSQLPIESQF ISSLKDNLNA EVVLGTVTNV KEACAWLGYT YLSIRMKLNP
LAYGIGWEEI IADPSLSLKQ RALVADAARS LDKAKMMRFD EKSGNFYCTE LGRVASHFYI
QYSSVETYNE MLKRHMNESE IINMVAHSSE FENIVVREEE QHELETLARS CCPLEVKGGP
SNKHGKISIL IQLYISRGSI DAFSLVSDAS YISASLARIM RALFEICLRK GWCEMTLFML
EYCKAVDRQL WPHQHPLRQF ERDLPSDILR KLEERRDDLD HLYEMEEKEI GALIRYNPGG
RLVKQHLGYF PSIQLAATVS PITRTVLKVD LLITPNFIWK DRFHGTALRW WILIEDTEND
YIYHSDLFTL TKRMARGEPQ KLSFTVPIFE PHPPQYYVHA VSDSWLHAET YFTISFHNLA
LPEARTSHTE LLDLKPLPVT SLGNKLYESL YKFSHFNPIQ TQIFHVLYHT DNNVLVGAPT
GSGKTISAEL AMLRLFSTQP DMKVVYIAPL KAIVRERMND WKKHLVAPLG KEMVEMTGDY
TPDLVALLSA DIIISTPEKW DGISRNWHTR SYVKKVGLVI LDEIHLLGAD RGPILEVIVS
RMRYISSQTE RSVRFVGLST ALANAGDLAD WLGVGEIGLF NFKPSVRPVP IEVHIQGYPG
KYYCPRMNSM NKPAYAAICT HSPTKPVLIF VSSRRQTRLT ALDLIQFAAS DEHPRQFLSV
SEEDLQMVLS QITDQNLRHT LQFGIGLHHA GLNDHDRSAV EELFTNNKIQ VLVSTSTLAW
GVNLPAHLVI IKGTEYFDGK TKRYVDFPLT EILQMMGRAG RPQFDQHGKA VILVHEPKKS
FYKKFLYEPF PVESSLKEKL HDHFNAEIVS GTIGNKEDAV HYLTWTYLFR RLMANPAYYG
LEGTQDETIC SYLSRLVQTT FEDLEDSGCL KVNEDSVEPT MLGTIASQYY LCYMTVSMFG
SNIGPDTSLE AFLHILAGAS EYDELPVRHN EENYNKTLSD RVRYPVDNNH LDDPHVKANL
LFQAHFSQLA LPISDYNTDL KSVLDQSIRI LQAMIDICAN SGWLSSSLTC MRLLQMVMQG
MWSDQDSSLW MIPCMNDLLL GSLTARGIHT LHQLLNLPRE TLQSVTENFP ASRLSQDLQR
FPRIQMNVRL QKKDSDGKKK PSTLEIRLEK TSKRNSSRAL APRFPKVKDE AWWLVLGDTS
TSELFAVKRV SFTGRLITRM ELPPNITSFQ DTKLILVSDC YLGFEQEHSI EQLARRG
