ID   DEXT_BACLI              Reviewed;          10 AA.
AC   C0HJE3;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   02-JUN-2021, entry version 6.
DE   RecName: Full=Dextranase {ECO:0000303|Ref.1};
DE            EC=3.2.1.11 {ECO:0000269|Ref.1};
DE   AltName: Full=Alpha-1,6-glucan-6-glucanohydrolase {ECO:0000250|UniProtKB:P39653};
DE   Flags: Fragment;
OS   Bacillus licheniformis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1402;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=KIBGE-IB25 {ECO:0000269|Ref.1};
RA   Zohra R.R., Sattar H., Karim A., Aman A., Qader S.A.;
RT   "Dextranase: isolation, purification and characterization of dextran
RT   degrading enzyme from Bacillus licheniformis KIBGE-IB25.";
RL   Submitted (AUG-2013) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in
CC         dextran.; EC=3.2.1.11; Evidence={ECO:0000269|Ref.1};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Note=Because of the indeterminate molecular weight of dextran, an
CC         approximate KM value of 0.3738 mg/ml was determined.
CC         {ECO:0000269|Ref.1};
CC       pH dependence:
CC         Optimum pH is 4.5. {ECO:0000269|Ref.1};
CC       Temperature dependence:
CC         Optimum temperature is 35 degrees Celsius. {ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 4.5,
CC       its MW is: 158 kDa. {ECO:0000269|Ref.1}.
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DR   BRENDA; 3.2.1.11; 669.
DR   GO; GO:0033904; F:dextranase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN           1..>10
FT                   /note="Dextranase"
FT                   /id="PRO_0000424175"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   10 AA;  1128 MW;  924BD23B5731B2D1 CRC64;
     AYTVTLYLQG