DEXT_TALMI
ID DEXT_TALMI Reviewed; 608 AA.
AC P48845;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Dextranase;
DE EC=3.2.1.11;
DE AltName: Full=Alpha-1,6-glucan-6-glucanohydrolase;
DE Flags: Precursor;
GN Name=DEX;
OS Talaromyces minioluteus (Filamentous fungus) (Penicillium minioluteum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC Talaromyces sect. Trachyspermi.
OX NCBI_TaxID=28574;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 35-50.
RC STRAIN=Sp. Dierckx / MUCL 38929;
RX PubMed=8905923;
RX DOI=10.1002/(sici)1097-0061(19960930)12:12<1187::aid-yea986>3.0.co;2-u;
RA Roca H., Garcia B.M., Rodriguez E., Mateu D., Coroas L., Cremata J.A.,
RA Garcia R., Pons T., Delgado J.;
RT "Cloning of the Penicillium minioluteum gene encoding dextranase and its
RT expression in Pichia pastoris.";
RL Yeast 12:1187-1200(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in
CC dextran.; EC=3.2.1.11;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: N-glycosylated.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 49 family. {ECO:0000305}.
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DR EMBL; L41562; AAB47720.1; -; Genomic_DNA.
DR PIR; S72177; S72177.
DR PDB; 1OGM; X-ray; 1.80 A; X=35-608.
DR PDB; 1OGO; X-ray; 1.65 A; X=35-608.
DR PDBsum; 1OGM; -.
DR PDBsum; 1OGO; -.
DR AlphaFoldDB; P48845; -.
DR SMR; P48845; -.
DR CAZy; GH49; Glycoside Hydrolase Family 49.
DR CLAE; DEX49A_PENMI; -.
DR BRENDA; 3.2.1.11; 7460.
DR EvolutionaryTrace; P48845; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033904; F:dextranase activity; IEA:UniProtKB-EC.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.160.20.10; -; 1.
DR Gene3D; 2.60.350.10; -; 1.
DR InterPro; IPR041402; B_solenoid_dext.
DR InterPro; IPR035953; Dextranase_N-ter.
DR InterPro; IPR005192; Glyco_hydro_49_C.
DR InterPro; IPR023226; Glyco_hydro_49_N_dom.
DR InterPro; IPR041274; IPU_b_solenoid.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF18841; B_solenoid_dext; 1.
DR Pfam; PF03718; Glyco_hydro_49; 1.
DR Pfam; PF17433; Glyco_hydro_49N; 1.
DR Pfam; PF18783; IPU_b_solenoid; 1.
DR SUPFAM; SSF101596; SSF101596; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycoprotein; Glycosidase;
KW Hydrolase; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..34
FT /evidence="ECO:0000269|PubMed:8905923"
FT /id="PRO_0000012213"
FT CHAIN 35..608
FT /note="Dextranase"
FT /id="PRO_0000012214"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 571
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 574
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT STRAND 43..48
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 72..80
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 96..99
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 126..129
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 132..142
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 144..150
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 159..161
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 172..175
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 181..185
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 192..198
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 199..201
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 209..234
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 238..240
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 246..248
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 254..257
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 259..262
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 264..270
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 272..277
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 288..290
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 301..304
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 308..312
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 314..316
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 322..327
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 329..331
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 341..345
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 351..353
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 357..360
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 365..373
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 375..377
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 383..386
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 388..390
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 392..402
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 419..429
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 431..433
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 440..448
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 450..452
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 454..456
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 464..476
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 484..487
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 489..493
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 497..500
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 504..518
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 520..522
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 524..528
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 531..544
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 558..560
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 568..577
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 584..586
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 591..593
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 595..597
FT /evidence="ECO:0007829|PDB:1OGO"
FT HELIX 599..601
FT /evidence="ECO:0007829|PDB:1OGO"
FT TURN 602..604
FT /evidence="ECO:0007829|PDB:1OGO"
FT STRAND 606..608
FT /evidence="ECO:0007829|PDB:1OGO"
SQ SEQUENCE 608 AA; 65961 MW; 6309B5FB372D381D CRC64;
MATMLKLLAL TLAISESAIG AVMHPPGNSH PGTHMGTTNN THCGADFCTW WHDSGEINTQ
TPVQPGNVRQ SHKYSVQVSL AGTNNFHDSF VYESIPRNGN GRIYAPTDPP NSNTLDSSVD
DGISIEPSIG LNMAWSQFEY SHDVDVKILA TDGSSLGSPS DVVIRPVSIS YAISQSDDGG
IVIRVPADAN GRKFSVEFKT DLYTFLSDGN EYVTSGGSVV GVEPTNALVI FASPFLPSGM
IPHMTPDNTQ TMTPGPINNG DWGAKSILYF PPGVYWMNQD QSGNSGKLGS NHIRLNSNTY
WVYLAPGAYV KGAIEYFTKQ NFYATGHGIL SGENYVYQAN AGDNYIAVKS DSTSLRMWWH
NNLGGGQTWY CVGPTINAPP FNTMDFNGNS GISSQISDYK QVGAFFFQTD GPEIYPNSVV
HDVFWHVNDD AIKIYYSGAS VSRATIWKCH NDPIIQMGWT SRDISGVTID TLNVIHTRYI
KSETVVPSAI IGASPFYASG MSPDSRKSIS MTVSNVVCEG LCPSLFRITP LQNYKNFVVK
NVAFPDGLQT NSIGTGESII PAASGLTMGL NISNWTVGGQ KVTMENFQAN SLGQFNIDGS
YWGEWQIS
