DF182_ARATH
ID DF182_ARATH Reviewed; 129 AA.
AC P82773; A0MFB0; Q1PE34; Q4VNZ1;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Defensin-like protein 182;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 59;
DE Short=Protein LCR59;
DE AltName: Full=Plant defensin 3.2;
DE Flags: Precursor;
GN Name=PDF3.2; Synonyms=LCR59; OrderedLocusNames=At4g30070;
GN ORFNames=F6G3.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000305};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 39-107, AND GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [5] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- CAUTION: Contains 8 disulfide bonds instead of the 4 disulfide bonds,
CC which are conserved features of the family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28659.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL078464; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161576; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE85716.1; -; Genomic_DNA.
DR EMBL; DQ446884; ABE66103.1; -; mRNA.
DR EMBL; DQ653236; ABK28659.1; ALT_SEQ; mRNA.
DR EMBL; AY803271; AAX39312.1; -; mRNA.
DR RefSeq; NP_567840.1; NM_119153.2.
DR AlphaFoldDB; P82773; -.
DR STRING; 3702.AT4G30070.1; -.
DR PaxDb; P82773; -.
DR PRIDE; P82773; -.
DR ProteomicsDB; 224242; -.
DR EnsemblPlants; AT4G30070.1; AT4G30070.1; AT4G30070.
DR GeneID; 829130; -.
DR Gramene; AT4G30070.1; AT4G30070.1; AT4G30070.
DR KEGG; ath:AT4G30070; -.
DR Araport; AT4G30070; -.
DR TAIR; locus:2126545; AT4G30070.
DR eggNOG; ENOG502SD1Z; Eukaryota.
DR HOGENOM; CLU_158287_1_1_1; -.
DR InParanoid; P82773; -.
DR OMA; NCDERCK; -.
DR OrthoDB; 1411313at2759; -.
DR PhylomeDB; P82773; -.
DR PRO; PR:P82773; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; P82773; baseline and differential.
DR Genevisible; P82773; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR InterPro; IPR039641; LCR.
DR PANTHER; PTHR36788; PTHR36788; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..129
FT /note="Defensin-like protein 182"
FT /id="PRO_0000017297"
FT DISULFID 29..70
FT /evidence="ECO:0000250"
FT DISULFID 36..55
FT /evidence="ECO:0000250"
FT DISULFID 39..64
FT /evidence="ECO:0000250"
FT DISULFID 43..66
FT /evidence="ECO:0000250"
FT DISULFID 83..129
FT /evidence="ECO:0000250"
FT DISULFID 94..114
FT /evidence="ECO:0000250"
FT DISULFID 99..123
FT /evidence="ECO:0000250"
FT DISULFID 103..125
FT /evidence="ECO:0000250"
SQ SEQUENCE 129 AA; 13863 MW; AD70E01548AC2C3F CRC64;
METVTSLVFI VNLLIIFTSV VNQARGDTCI DGLGYCNNCD ERCKAKHGPS SESSCDRSVG
VPLCKCYYEC ESPPSPPAPP KKCDGGAGIC SQRCQGQCCD MNCAQKYIGG HGFCNTLGTF
SFCQCEYPC
