DF193_ARATH
ID DF193_ARATH Reviewed; 90 AA.
AC O22866; Q67YC6; Q6ZZT9;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Defensin-like protein 193;
DE AltName: Full=Trypsin inhibitor ATTI-2;
DE Flags: Precursor;
GN Name=ATTI2; OrderedLocusNames=At2g43520; ORFNames=T01O24.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0,
RC cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0,
RC cv. Sah-0, cv. Ta-0, cv. Wassilewskija, cv. Wei-0, and cv. Wil-2;
RC TISSUE=Leaf;
RX PubMed=15082560; DOI=10.1534/genetics.166.3.1419;
RA Clauss M.J., Mitchell-Olds T.;
RT "Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin
RT inhibitor genes.";
RL Genetics 166:1419-1436(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC (RTI/MTI-2) subfamily. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to be a protease inhibitor.
CC {ECO:0000305|PubMed:15082560}.
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DR EMBL; AJ632250; CAG15156.1; -; Genomic_DNA.
DR EMBL; AJ632251; CAG15161.1; -; Genomic_DNA.
DR EMBL; AJ632252; CAG15166.1; -; Genomic_DNA.
DR EMBL; AJ632253; CAG15171.1; -; Genomic_DNA.
DR EMBL; AJ632254; CAG15176.1; -; Genomic_DNA.
DR EMBL; AJ632255; CAG15182.1; -; Genomic_DNA.
DR EMBL; AJ632256; CAG15188.1; -; Genomic_DNA.
DR EMBL; AJ632257; CAG15194.1; -; Genomic_DNA.
DR EMBL; AJ632258; CAG15199.1; -; Genomic_DNA.
DR EMBL; AJ632259; CAG15204.1; -; Genomic_DNA.
DR EMBL; AJ632260; CAG15209.1; -; Genomic_DNA.
DR EMBL; AJ632261; CAG15214.1; -; Genomic_DNA.
DR EMBL; AJ632262; CAG15219.1; -; Genomic_DNA.
DR EMBL; AJ632263; CAG15224.1; -; Genomic_DNA.
DR EMBL; AJ632264; CAG15229.1; -; Genomic_DNA.
DR EMBL; AJ632265; CAG15234.1; -; Genomic_DNA.
DR EMBL; AJ632266; CAG15239.1; -; Genomic_DNA.
DR EMBL; AC002335; AAB64324.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10285.1; -; Genomic_DNA.
DR EMBL; AK175705; BAD43468.1; -; mRNA.
DR EMBL; AK176542; BAD44305.1; -; mRNA.
DR EMBL; AK176838; BAD44601.1; -; mRNA.
DR EMBL; AY085600; AAM62821.1; -; mRNA.
DR PIR; B84867; B84867.
DR RefSeq; NP_181880.1; NM_129913.3.
DR AlphaFoldDB; O22866; -.
DR SMR; O22866; -.
DR STRING; 3702.AT2G43520.1; -.
DR PaxDb; O22866; -.
DR PRIDE; O22866; -.
DR ProteomicsDB; 224184; -.
DR EnsemblPlants; AT2G43520.1; AT2G43520.1; AT2G43520.
DR GeneID; 818953; -.
DR Gramene; AT2G43520.1; AT2G43520.1; AT2G43520.
DR KEGG; ath:AT2G43520; -.
DR Araport; AT2G43520; -.
DR TAIR; locus:2058256; AT2G43520.
DR HOGENOM; CLU_181760_0_0_1; -.
DR InParanoid; O22866; -.
DR OMA; TICYIRC; -.
DR OrthoDB; 1807866at2759; -.
DR PhylomeDB; O22866; -.
DR PRO; PR:O22866; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22866; baseline and differential.
DR Genevisible; O22866; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISS:TAIR.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; TAS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 3: Inferred from homology;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..90
FT /note="Defensin-like protein 193"
FT /id="PRO_0000031094"
FT SITE 48..49
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT DISULFID 32..86
FT /evidence="ECO:0000250"
FT DISULFID 45..69
FT /evidence="ECO:0000250"
FT DISULFID 54..81
FT /evidence="ECO:0000250"
FT DISULFID 58..83
FT /evidence="ECO:0000250"
FT VARIANT 3
FT /note="M -> T (in strain: cv. Sah-0)"
SQ SEQUENCE 90 AA; 9660 MW; 405F8A927F5A9F6A CRC64;
MAMKSVSTLA VFAILFLVIV EMPEIKAQGS KCLKEYGGNV GFSYCAPRIF PSFCYRNCRK
NKGAKGGRCR SGGAGAGSMI CLCDYCSDKP
