DF194_ARATH
ID DF194_ARATH Reviewed; 83 AA.
AC O22867; B9DGH7; F4IR67; Q6ZZP9; Q6ZZQ4; Q6ZZQ9; Q6ZZR4; Q6ZZR9; Q6ZZT8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Defensin-like protein 194;
DE AltName: Full=Trypsin inhibitor ATTI-3;
DE Flags: Precursor;
GN Name=ATTI3; OrderedLocusNames=At2g43530; ORFNames=T01O24.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0,
RC cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0,
RC cv. Sah-0, cv. Ta-0, cv. Wassilewskija, cv. Wei-0, and cv. Wil-2;
RC TISSUE=Leaf;
RX PubMed=15082560; DOI=10.1534/genetics.166.3.1419;
RA Clauss M.J., Mitchell-Olds T.;
RT "Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin
RT inhibitor genes.";
RL Genetics 166:1419-1436(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC (RTI/MTI-2) subfamily. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to be a protease inhibitor.
CC {ECO:0000305|PubMed:15082560}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64336.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAH19844.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAG15162.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ632250; CAG15157.1; -; Genomic_DNA.
DR EMBL; AJ632251; CAG15162.1; ALT_INIT; Genomic_DNA.
DR EMBL; AJ632252; CAG15167.1; -; Genomic_DNA.
DR EMBL; AJ632253; CAG15172.1; -; Genomic_DNA.
DR EMBL; AJ632254; CAG15177.1; -; Genomic_DNA.
DR EMBL; AJ632255; CAG15183.1; -; Genomic_DNA.
DR EMBL; AJ632256; CAG15189.1; -; Genomic_DNA.
DR EMBL; AJ632257; CAG15195.1; -; Genomic_DNA.
DR EMBL; AJ632258; CAG15200.1; -; Genomic_DNA.
DR EMBL; AJ632259; CAG15205.1; -; Genomic_DNA.
DR EMBL; AJ632260; CAG15210.1; -; Genomic_DNA.
DR EMBL; AJ632261; CAG15215.1; -; Genomic_DNA.
DR EMBL; AJ632262; CAG15220.1; -; Genomic_DNA.
DR EMBL; AJ632263; CAG15225.1; -; Genomic_DNA.
DR EMBL; AJ632264; CAG15230.1; -; Genomic_DNA.
DR EMBL; AJ632265; CAG15235.1; -; Genomic_DNA.
DR EMBL; AJ632266; CAG15240.1; -; Genomic_DNA.
DR EMBL; AC002335; AAB64336.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC10286.2; -; Genomic_DNA.
DR EMBL; BX819403; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK317158; BAH19844.1; ALT_INIT; mRNA.
DR PIR; C84867; C84867.
DR RefSeq; NP_181881.2; NM_129914.4.
DR AlphaFoldDB; O22867; -.
DR SMR; O22867; -.
DR STRING; 3702.AT2G43530.1; -.
DR PaxDb; O22867; -.
DR PRIDE; O22867; -.
DR ProteomicsDB; 224185; -.
DR EnsemblPlants; AT2G43530.1; AT2G43530.1; AT2G43530.
DR GeneID; 818954; -.
DR Gramene; AT2G43530.1; AT2G43530.1; AT2G43530.
DR KEGG; ath:AT2G43530; -.
DR Araport; AT2G43530; -.
DR HOGENOM; CLU_181760_0_0_1; -.
DR OMA; CMARIYP; -.
DR OrthoDB; 1822151at2759; -.
DR PhylomeDB; O22867; -.
DR PRO; PR:O22867; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22867; baseline and differential.
DR Genevisible; O22867; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 3: Inferred from homology;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..83
FT /note="Defensin-like protein 194"
FT /id="PRO_0000031095"
FT SITE 47..48
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT DISULFID 32..78
FT /evidence="ECO:0000250"
FT DISULFID 44..68
FT /evidence="ECO:0000250"
FT DISULFID 53..73
FT /evidence="ECO:0000250"
FT DISULFID 57..75
FT /evidence="ECO:0000250"
FT VARIANT 11
FT /note="I -> T (in strain: cv. Cvi-1 and cv. Nd-1)"
FT VARIANT 20
FT /note="S -> Y (in strain: cv. Nok-0)"
FT VARIANT 30
FT /note="K -> S (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
FT VARIANT 59
FT /note="F -> S (in strain: cv. Cvi-1, cv. Di-0, cv. Fe-1a,
FT cv. Goe-0, cv. Ita-0, cv. Kas-1, cv. Landsberg erecta, cv.
FT Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0, cv. Sah-0, cv. Ta-0,
FT cv. Wei-0 and cv. Wil-2)"
FT CONFLICT 80
FT /note="D -> H (in Ref. 4; BX819403)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 83 AA; 9408 MW; A7130584F290F934 CRC64;
MAMKSVSNFA IFLILFLVTS EISEIEAKDK ECLKGYIDAP LSYCMARIYP SLCYRNCRFY
KGAKGGKCDG LKCFCDFCSD KPF
