DF196_ARATH
ID DF196_ARATH Reviewed; 97 AA.
AC Q8RYE7; Q6ZZS3;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Defensin-like protein 196;
DE AltName: Full=Trypsin inhibitor ATTI-4;
DE Flags: Precursor;
GN Name=ATTI4; OrderedLocusNames=At2g43535; ORFNames=T1O24.45;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0,
RC cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0,
RC cv. Ta-0, cv. Wassilewskija, cv. Wei-0, and cv. Wil-2; TISSUE=Leaf;
RX PubMed=15082560; DOI=10.1534/genetics.166.3.1419;
RA Clauss M.J., Mitchell-Olds T.;
RT "Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin
RT inhibitor genes.";
RL Genetics 166:1419-1436(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC (RTI/MTI-2) subfamily. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to be a protease inhibitor.
CC {ECO:0000305|PubMed:15082560}.
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DR EMBL; AJ632250; CAG15158.1; -; Genomic_DNA.
DR EMBL; AJ632252; CAG15168.1; -; Genomic_DNA.
DR EMBL; AJ632253; CAG15173.1; -; Genomic_DNA.
DR EMBL; AJ632254; CAG15178.1; -; Genomic_DNA.
DR EMBL; AJ632255; CAG15184.1; -; Genomic_DNA.
DR EMBL; AJ632256; CAG15190.1; -; Genomic_DNA.
DR EMBL; AJ632257; CAG15196.1; -; Genomic_DNA.
DR EMBL; AJ632258; CAG15201.1; -; Genomic_DNA.
DR EMBL; AJ632259; CAG15206.1; -; Genomic_DNA.
DR EMBL; AJ632260; CAG15211.1; -; Genomic_DNA.
DR EMBL; AJ632261; CAG15216.1; -; Genomic_DNA.
DR EMBL; AJ632262; CAG15221.1; -; Genomic_DNA.
DR EMBL; AJ632263; CAG15226.1; -; Genomic_DNA.
DR EMBL; AJ632264; CAG15231.1; -; Genomic_DNA.
DR EMBL; AJ632265; CAG15236.1; -; Genomic_DNA.
DR EMBL; AJ632266; CAG15241.1; -; Genomic_DNA.
DR EMBL; AC002335; AAM14812.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10287.1; -; Genomic_DNA.
DR EMBL; BT009648; AAP75798.1; -; mRNA.
DR EMBL; AK228120; BAF00078.1; -; mRNA.
DR EMBL; AY088677; AAM66999.1; -; mRNA.
DR RefSeq; NP_566000.1; NM_129915.4.
DR AlphaFoldDB; Q8RYE7; -.
DR SMR; Q8RYE7; -.
DR STRING; 3702.AT2G43535.1; -.
DR MEROPS; I18.001; -.
DR MEROPS; I18.002; -.
DR PaxDb; Q8RYE7; -.
DR PRIDE; Q8RYE7; -.
DR ProteomicsDB; 224626; -.
DR EnsemblPlants; AT2G43535.1; AT2G43535.1; AT2G43535.
DR GeneID; 818955; -.
DR Gramene; AT2G43535.1; AT2G43535.1; AT2G43535.
DR KEGG; ath:AT2G43535; -.
DR Araport; AT2G43535; -.
DR TAIR; locus:505006315; AT2G43535.
DR HOGENOM; CLU_181760_0_0_1; -.
DR OMA; CRHESGQ; -.
DR OrthoDB; 1828022at2759; -.
DR PhylomeDB; Q8RYE7; -.
DR PRO; PR:Q8RYE7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8RYE7; baseline and differential.
DR Genevisible; Q8RYE7; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 3: Inferred from homology;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..97
FT /note="Defensin-like protein 196"
FT /id="PRO_0000379690"
FT SITE 49..50
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT DISULFID 33..85
FT /evidence="ECO:0000250"
FT DISULFID 46..70
FT /evidence="ECO:0000250"
FT DISULFID 55..80
FT /evidence="ECO:0000250"
FT DISULFID 59..82
FT /evidence="ECO:0000250"
FT VARIANT 3
FT /note="K -> T (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
FT VARIANT 6
FT /note="A -> V (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
FT VARIANT 14
FT /note="I -> T (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
FT VARIANT 77
FT /note="K -> N (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
FT VARIANT 87
FT /note="N -> K (in strain: cv. Cvi-1, cv. Fe-1a, cv. Nd-1
FT and cv. Wei-0)"
SQ SEQUENCE 97 AA; 10671 MW; 62C66F740E8BF8C9 CRC64;
MAKMSALSIF AIFIILVLVI FEIPEIEAHD SECLKEYGGD VGFGFCAPKI FPTICYRNCQ
KDKGANGGKC LWGEGGKVKC LCDFCSNESF NQFISLT
