DF197_ARATH
ID DF197_ARATH Reviewed; 89 AA.
AC O22869; F4IR70; Q6ZZS7; Q6ZZU1; Q8GWN0;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=Defensin-like protein 197;
DE AltName: Full=Trypsin inhibitor ATTI-6;
DE Flags: Precursor;
GN Name=ATTI6; OrderedLocusNames=At2g43550; ORFNames=T01O24.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0,
RC cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0,
RC cv. Sah-0, cv. Ta-0, cv. Wassilewskija, cv. Wei-0, and cv. Wil-2;
RC TISSUE=Leaf;
RX PubMed=15082560; DOI=10.1534/genetics.166.3.1419;
RA Clauss M.J., Mitchell-Olds T.;
RT "Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin
RT inhibitor genes.";
RL Genetics 166:1419-1436(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC (RTI/MTI-2) subfamily. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to be a protease inhibitor.
CC {ECO:0000305|PubMed:15082560}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64322.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ632250; CAG15159.1; -; Genomic_DNA.
DR EMBL; AJ632251; CAG15164.1; -; Genomic_DNA.
DR EMBL; AJ632252; CAG15169.1; -; Genomic_DNA.
DR EMBL; AJ632253; CAG26677.1; -; Genomic_DNA.
DR EMBL; AJ632254; CAG15180.1; -; Genomic_DNA.
DR EMBL; AJ632255; CAG15186.1; -; Genomic_DNA.
DR EMBL; AJ632256; CAG15192.1; -; Genomic_DNA.
DR EMBL; AJ632257; CAG15197.1; -; Genomic_DNA.
DR EMBL; AJ632258; CAG15202.1; -; Genomic_DNA.
DR EMBL; AJ632259; CAG15207.1; -; Genomic_DNA.
DR EMBL; AJ632260; CAG15212.1; -; Genomic_DNA.
DR EMBL; AJ632261; CAG15217.1; -; Genomic_DNA.
DR EMBL; AJ632262; CAG15222.1; -; Genomic_DNA.
DR EMBL; AJ632263; CAG15227.1; -; Genomic_DNA.
DR EMBL; AJ632264; CAG15232.1; -; Genomic_DNA.
DR EMBL; AJ632265; CAG15237.1; -; Genomic_DNA.
DR EMBL; AJ632266; CAG15242.1; -; Genomic_DNA.
DR EMBL; AC002335; AAB64322.2; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC10289.2; -; Genomic_DNA.
DR EMBL; AK118750; BAC43343.1; -; mRNA.
DR PIR; E84867; E84867.
DR RefSeq; NP_566002.2; NM_129917.3.
DR AlphaFoldDB; O22869; -.
DR SMR; O22869; -.
DR STRING; 3702.AT2G43550.1; -.
DR PaxDb; O22869; -.
DR PRIDE; O22869; -.
DR ProteomicsDB; 224186; -.
DR EnsemblPlants; AT2G43550.1; AT2G43550.1; AT2G43550.
DR GeneID; 818957; -.
DR Gramene; AT2G43550.1; AT2G43550.1; AT2G43550.
DR KEGG; ath:AT2G43550; -.
DR Araport; AT2G43550; -.
DR HOGENOM; CLU_181760_0_0_1; -.
DR InParanoid; O22869; -.
DR OMA; ICVVEYG; -.
DR OrthoDB; 1570782at2759; -.
DR PhylomeDB; O22869; -.
DR PRO; PR:O22869; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22869; baseline and differential.
DR Genevisible; O22869; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 3: Inferred from homology;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..89
FT /note="Defensin-like protein 197"
FT /id="PRO_0000031096"
FT SITE 49..50
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT DISULFID 33..86
FT /evidence="ECO:0000250"
FT DISULFID 46..70
FT /evidence="ECO:0000250"
FT DISULFID 55..81
FT /evidence="ECO:0000250"
FT DISULFID 59..83
FT /evidence="ECO:0000250"
FT VARIANT 3
FT /note="F -> S (in strain: cv. Cvi-1, cv. Fe-1a, cv. Goe-0,
FT cv. Ita-0, cv. Nd-1 and cv. Wei-0)"
FT VARIANT 8
FT /note="L -> F (in strain: cv. Cvi-1, cv. Fe-1a, cv. Goe-0,
FT cv. Ita-0, cv. Nd-1 and cv. Wei-0)"
FT VARIANT 13
FT /note="F -> I (in strain: cv. Cvi-1, cv. Fe-1a, cv. Goe-0,
FT cv. Ita-0, cv. Nd-1 and cv. Wei-0)"
FT VARIANT 19
FT /note="Missing (in strain: cv. Col-1)"
FT CONFLICT 3
FT /note="F -> L (in Ref. 4; BAC43343)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 89 AA; 10192 MW; C0964810F33099A3 CRC64;
MKFVSVFLVL FIFFLVVLEA PEKIEAKDDK FICVVEYGGD VGPTFCNPKF FPTLCRQNCR
SFKGAKGGKC VKQPKHKHIK CFCDYCKDD
