DF1_ARATH
ID DF1_ARATH Reviewed; 603 AA.
AC Q9C6K3;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=Trihelix transcription factor DF1 {ECO:0000303|PubMed:22735692};
GN Name=DF1 {ECO:0000303|PubMed:22735692};
GN OrderedLocusNames=At1g76880 {ECO:0000312|Araport:AT1G76880};
GN ORFNames=F7O12.5 {ECO:0000312|EMBL:AAG51144.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22735692; DOI=10.1039/c2mb25096a;
RA Vasilevski A., Giorgi F.M., Bertinetti L., Usadel B.;
RT "LASSO modeling of the Arabidopsis thaliana seed/seedling transcriptome: a
RT model case for detection of novel mucilage and pectin metabolism genes.";
RL Mol. Biosyst. 8:2566-2574(2012).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25658798; DOI=10.3390/ijms16023452;
RA Voiniciuc C., Yang B., Schmidt M.H.-W., Guenl M., Usadel B.;
RT "Starting to gel: how Arabidopsis seed coat epidermal cells produce
RT specialized secondary cell walls.";
RL Int. J. Mol. Sci. 16:3452-3473(2015).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=29439132; DOI=10.1242/dev.159707;
RA Shibata M., Breuer C., Kawamura A., Clark N.M., Rymen B., Braidwood L.,
RA Morohashi K., Busch W., Benfey P.N., Sozzani R., Sugimoto K.;
RT "GTL1 and DF1 regulate root hair growth through transcriptional repression
RT of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis.";
RL Development 145:0-0(2018).
CC -!- FUNCTION: Transcription repressor that negatively regulates root hair
CC growth by directly binding RSL4 promoter and repressing RSL4 expression
CC (PubMed:29439132). Required for the synthesis of seed coat mucilage
CC (PubMed:22735692, PubMed:25658798). {ECO:0000269|PubMed:22735692,
CC ECO:0000269|PubMed:25658798, ECO:0000269|PubMed:29439132}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:29439132}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutant seedlings gtl1-1 and df1-1 exhibit
CC increased root hair length (PubMed:29439132). Reduced amount of seed
CC coat mucilage (PubMed:22735692, PubMed:25658798).
CC {ECO:0000269|PubMed:22735692, ECO:0000269|PubMed:25658798,
CC ECO:0000269|PubMed:29439132}.
CC -!- MISCELLANEOUS: Plants overexpressing DF1 exhibit very short root hairs.
CC {ECO:0000269|PubMed:29439132}.
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DR EMBL; AC079283; AAG51144.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35898.1; -; Genomic_DNA.
DR PIR; F96797; F96797.
DR RefSeq; NP_177814.1; NM_106339.4.
DR AlphaFoldDB; Q9C6K3; -.
DR SMR; Q9C6K3; -.
DR IntAct; Q9C6K3; 4.
DR STRING; 3702.AT1G76880.1; -.
DR PaxDb; Q9C6K3; -.
DR PRIDE; Q9C6K3; -.
DR ProteomicsDB; 189103; -.
DR EnsemblPlants; AT1G76880.1; AT1G76880.1; AT1G76880.
DR GeneID; 844023; -.
DR Gramene; AT1G76880.1; AT1G76880.1; AT1G76880.
DR KEGG; ath:AT1G76880; -.
DR Araport; AT1G76880; -.
DR TAIR; locus:2036267; AT1G76880.
DR eggNOG; KOG4282; Eukaryota.
DR HOGENOM; CLU_013796_1_1_1; -.
DR InParanoid; Q9C6K3; -.
DR OMA; AKTDNGD; -.
DR OrthoDB; 896806at2759; -.
DR PhylomeDB; Q9C6K3; -.
DR PRO; PR:Q9C6K3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6K3; differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0019760; P:glucosinolate metabolic process; IMP:TAIR.
DR GO; GO:0010192; P:mucilage biosynthetic process; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR044822; Myb_DNA-bind_4.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF13837; Myb_DNA-bind_4; 2.
DR SMART; SM00717; SANT; 2.
DR PROSITE; PS50090; MYB_LIKE; 2.
PE 4: Predicted;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..603
FT /note="Trihelix transcription factor DF1"
FT /id="PRO_0000450106"
FT DOMAIN 60..118
FT /note="Myb-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT DOMAIN 401..465
FT /note="Myb-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT REGION 149..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..377
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..559
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 560..587
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..603
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 603 AA; 67879 MW; 3786E91295D5F42E CRC64;
MMQLGGGTPT TTAAATTVTT ATAPPPQSNN NDSAATEAAA AAVGAFEVSE EMHDRGFGGN
RWPRQETLAL LKIRSDMGIA FRDASVKGPL WEEVSRKMAE HGYIRNAKKC KEKFENVYKY
HKRTKEGRTG KSEGKTYRFF DQLEALESQS TTSLHHHQQQ TPLRPQQNNN NNNNNNNNSS
IFSTPPPVTT VMPTLPSSSI PPYTQQINVP SFPNISGDFL SDNSTSSSSS YSTSSDMEMG
GGTATTRKKR KRKWKVFFER LMKQVVDKQE ELQRKFLEAV EKREHERLVR EESWRVQEIA
RINREHEILA QERSMSAAKD AAVMAFLQKL SEKQPNQPQP QPQPQQVRPS MQLNNNNQQQ
PPQRSPPPQP PAPLPQPIQA VVSTLDTTKT DNGGDQNMTP AASASSSRWP KVEIEALIKL
RTNLDSKYQE NGPKGPLWEE ISAGMRRLGF NRNSKRCKEK WENINKYFKK VKESNKKRPE
DSKTCPYFHQ LDALYRERNK FHSNNNIAAS SSSSGLVKPD NSVPLMVQPE QQWPPAVTTA
TTTPAAAQPD QQSQPSEQNF DDEEGTDEEY DDEDEEEENE EEEGGEFELV PSNNNNNKTT
NNL
