ADA1B_HUMAN
ID ADA1B_HUMAN Reviewed; 520 AA.
AC P35368; B0LPE1;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 3.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Alpha-1B adrenergic receptor;
DE AltName: Full=Alpha-1B adrenoreceptor;
DE Short=Alpha-1B adrenoceptor;
GN Name=ADRA1B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1328250; DOI=10.1016/s0021-9258(19)36703-1;
RA Ramarao C.S., Denker J.M., Perez D.M., Gaivin R.J., Riek R.P., Graham R.M.;
RT "Genomic organization and expression of the human alpha 1B-adrenergic
RT receptor.";
RL J. Biol. Chem. 267:21936-21945(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=8183249;
RA Forray C., Bard J.A., Wetzel J.M., Chiu G., Shapiro E., Tang R., Lepor H.,
RA Hartig P.R., Weinshank R.L., Branchek T.A., Gluchowski C.;
RT "The alpha 1-adrenergic receptor that mediates smooth muscle contraction in
RT human prostate has the pharmacological properties of the cloned human alpha
RT 1c subtype.";
RL Mol. Pharmacol. 45:703-708(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7815325;
RA Schwinn D.A., Johnston G.I., Page S.O., Mosley M.J., Wilson K.H.,
RA Worman N.P., Campbell S., Fidock M.D., Furness L.M., Parry-Smith D.J.,
RA Peter B., Bailey D.S.;
RT "Cloning and pharmacological characterization of human alpha-1 adrenergic
RT receptors: sequence corrections and direct comparison with other species
RT homologues.";
RL J. Pharmacol. Exp. Ther. 272:134-142(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RG NIEHS SNPs program;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=18802028; DOI=10.1161/circresaha.108.176024;
RA Wright C.D., Chen Q., Baye N.L., Huang Y., Healy C.L., Kasinathan S.,
RA O'Connell T.D.;
RT "Nuclear alpha1-adrenergic receptors signal activated ERK localization to
RT caveolae in adult cardiac myocytes.";
RL Circ. Res. 103:992-1000(2008).
RN [7]
RP SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, AND MUTAGENESIS OF
RP 368-ARG--ARG-380.
RX PubMed=22120526; DOI=10.1016/j.cellsig.2011.11.014;
RA Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D.;
RT "Nuclear localization drives alpha1-adrenergic receptor oligomerization and
RT signaling in cardiac myocytes.";
RL Cell. Signal. 24:794-802(2012).
RN [8]
RP INTERACTION WITH CAVIN4, AND SUBCELLULAR LOCATION.
RX PubMed=24567387; DOI=10.1073/pnas.1315359111;
RA Ogata T., Naito D., Nakanishi N., Hayashi Y.K., Taniguchi T., Miyagawa K.,
RA Hamaoka T., Maruyama N., Matoba S., Ikeda K., Yamada H., Oh H., Ueyama T.;
RT "MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric
RT cardiac hypertrophy induced by alpha1-adrenergic receptors.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:3811-3816(2014).
CC -!- FUNCTION: This alpha-adrenergic receptor mediates its action by
CC association with G proteins that activate a phosphatidylinositol-
CC calcium second messenger system. Its effect is mediated by G(q) and
CC G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate
CC phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
CC {ECO:0000269|PubMed:18802028, ECO:0000269|PubMed:22120526}.
CC -!- SUBUNIT: Homo- and heterooligomer. Heterooligomerizes with ADRA1B
CC homooligomers in cardiac myocytes (PubMed:22120526). Interacts with
CC CAVIN4 (PubMed:24567387). {ECO:0000269|PubMed:22120526,
CC ECO:0000269|PubMed:24567387}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane; Multi-pass membrane protein.
CC Cell membrane {ECO:0000269|PubMed:24567387}; Multi-pass membrane
CC protein {ECO:0000255}. Cytoplasm {ECO:0000269|PubMed:24567387}.
CC Membrane, caveola {ECO:0000269|PubMed:24567387}. Note=Location at the
CC nuclear membrane facilitates heterooligomerization and regulates ERK-
CC mediated signaling in cardiac myocytes. signaling in cardiac myocytes.
CC Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of
CC cardiac myocytes.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Adrenergic receptor subfamily. ADRA1B sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M99589; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; M99590; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U03865; AAB60352.1; -; mRNA.
DR EMBL; L31773; AAB59485.1; -; mRNA.
DR EMBL; EU332831; ABY87520.1; -; Genomic_DNA.
DR EMBL; BC136568; AAI36569.1; -; mRNA.
DR EMBL; BC136569; AAI36570.1; -; mRNA.
DR CCDS; CCDS4347.1; -.
DR PIR; A45121; A45121.
DR RefSeq; NP_000670.1; NM_000679.3.
DR AlphaFoldDB; P35368; -.
DR SMR; P35368; -.
DR BioGRID; 106657; 6.
DR DIP; DIP-33399N; -.
DR IntAct; P35368; 9.
DR MINT; P35368; -.
DR STRING; 9606.ENSP00000306662; -.
DR BindingDB; P35368; -.
DR ChEMBL; CHEMBL232; -.
DR DrugBank; DB01614; Acepromazine.
DR DrugBank; DB00346; Alfuzosin.
DR DrugBank; DB00321; Amitriptyline.
DR DrugBank; DB00543; Amoxapine.
DR DrugBank; DB00182; Amphetamine.
DR DrugBank; DB09229; Aranidipine.
DR DrugBank; DB01238; Aripiprazole.
DR DrugBank; DB14185; Aripiprazole lauroxil.
DR DrugBank; DB09204; Arotinolol.
DR DrugBank; DB09128; Brexpiprazole.
DR DrugBank; DB01200; Bromocriptine.
DR DrugBank; DB00490; Buspirone.
DR DrugBank; DB00248; Cabergoline.
DR DrugBank; DB01136; Carvedilol.
DR DrugBank; DB00477; Chlorpromazine.
DR DrugBank; DB09202; Cirazoline.
DR DrugBank; DB00575; Clonidine.
DR DrugBank; DB00363; Clozapine.
DR DrugBank; DB00298; Dapiprazole.
DR DrugBank; DB01151; Desipramine.
DR DrugBank; DB01576; Dextroamphetamine.
DR DrugBank; DB11273; Dihydroergocornine.
DR DrugBank; DB13345; Dihydroergocristine.
DR DrugBank; DB00320; Dihydroergotamine.
DR DrugBank; DB11278; DL-Methylephedrine.
DR DrugBank; DB00841; Dobutamine.
DR DrugBank; DB09167; Dosulepin.
DR DrugBank; DB00590; Doxazosin.
DR DrugBank; DB01142; Doxepin.
DR DrugBank; DB04855; Dronedarone.
DR DrugBank; DB06262; Droxidopa.
DR DrugBank; DB05492; Epicept NP-1.
DR DrugBank; DB00668; Epinephrine.
DR DrugBank; DB01049; Ergoloid mesylate.
DR DrugBank; DB00696; Ergotamine.
DR DrugBank; DB01175; Escitalopram.
DR DrugBank; DB09194; Etoperidone.
DR DrugBank; DB00800; Fenoldopam.
DR DrugBank; DB00458; Imipramine.
DR DrugBank; DB11577; Indigotindisulfonic acid.
DR DrugBank; DB00598; Labetalol.
DR DrugBank; DB09195; Lorpiprazole.
DR DrugBank; DB00408; Loxapine.
DR DrugBank; DB00934; Maprotiline.
DR DrugBank; DB01365; Mephentermine.
DR DrugBank; DB01403; Methotrimeprazine.
DR DrugBank; DB00723; Methoxamine.
DR DrugBank; DB06148; Mianserin.
DR DrugBank; DB00211; Midodrine.
DR DrugBank; DB00370; Mirtazapine.
DR DrugBank; DB00745; Modafinil.
DR DrugBank; DB09205; Moxisylyte.
DR DrugBank; DB01149; Nefazodone.
DR DrugBank; DB00622; Nicardipine.
DR DrugBank; DB00368; Norepinephrine.
DR DrugBank; DB00540; Nortriptyline.
DR DrugBank; DB06229; Ocaperidone.
DR DrugBank; DB00334; Olanzapine.
DR DrugBank; DB00935; Oxymetazoline.
DR DrugBank; DB01267; Paliperidone.
DR DrugBank; DB00715; Paroxetine.
DR DrugBank; DB01186; Pergolide.
DR DrugBank; DB01608; Periciazine.
DR DrugBank; DB08922; Perospirone.
DR DrugBank; DB01579; Phendimetrazine.
DR DrugBank; DB00925; Phenoxybenzamine.
DR DrugBank; DB00692; Phentolamine.
DR DrugBank; DB00388; Phenylephrine.
DR DrugBank; DB09286; Pipamperone.
DR DrugBank; DB06153; Pizotifen.
DR DrugBank; DB00457; Prazosin.
DR DrugBank; DB00433; Prochlorperazine.
DR DrugBank; DB01069; Promethazine.
DR DrugBank; DB01224; Quetiapine.
DR DrugBank; DB00908; Quinidine.
DR DrugBank; DB05469; R450.
DR DrugBank; DB11124; Racepinephrine.
DR DrugBank; DB00243; Ranolazine.
DR DrugBank; DB00734; Risperidone.
DR DrugBank; DB00268; Ropinirole.
DR DrugBank; DB06144; Sertindole.
DR DrugBank; DB06207; Silodosin.
DR DrugBank; DB09203; Synephrine.
DR DrugBank; DB00706; Tamsulosin.
DR DrugBank; DB01162; Terazosin.
DR DrugBank; DB06764; Tetryzoline.
DR DrugBank; DB01622; Thioproperazine.
DR DrugBank; DB00679; Thioridazine.
DR DrugBank; DB13025; Tiapride.
DR DrugBank; DB00697; Tizanidine.
DR DrugBank; DB00726; Trimipramine.
DR DrugBank; DB00661; Verapamil.
DR DrugBank; DB06694; Xylometazoline.
DR DrugBank; DB00246; Ziprasidone.
DR DrugCentral; P35368; -.
DR GuidetoPHARMACOLOGY; 23; -.
DR GlyGen; P35368; 4 sites.
DR iPTMnet; P35368; -.
DR PhosphoSitePlus; P35368; -.
DR BioMuta; ADRA1B; -.
DR DMDM; 116241241; -.
DR MassIVE; P35368; -.
DR MaxQB; P35368; -.
DR PaxDb; P35368; -.
DR PeptideAtlas; P35368; -.
DR PRIDE; P35368; -.
DR ProteomicsDB; 55037; -.
DR Antibodypedia; 16635; 510 antibodies from 38 providers.
DR DNASU; 147; -.
DR Ensembl; ENST00000306675.5; ENSP00000306662.3; ENSG00000170214.5.
DR GeneID; 147; -.
DR KEGG; hsa:147; -.
DR MANE-Select; ENST00000306675.5; ENSP00000306662.3; NM_000679.4; NP_000670.1.
DR UCSC; uc003lxt.2; human.
DR CTD; 147; -.
DR DisGeNET; 147; -.
DR GeneCards; ADRA1B; -.
DR HGNC; HGNC:278; ADRA1B.
DR HPA; ENSG00000170214; Tissue enhanced (brain, liver, lymphoid tissue).
DR MIM; 104220; gene.
DR neXtProt; NX_P35368; -.
DR OpenTargets; ENSG00000170214; -.
DR PharmGKB; PA33; -.
DR VEuPathDB; HostDB:ENSG00000170214; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000156944; -.
DR HOGENOM; CLU_009579_11_6_1; -.
DR InParanoid; P35368; -.
DR OMA; STRAKGH; -.
DR OrthoDB; 1095345at2759; -.
DR PhylomeDB; P35368; -.
DR TreeFam; TF331895; -.
DR PathwayCommons; P35368; -.
DR Reactome; R-HSA-390696; Adrenoceptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR Reactome; R-HSA-416482; G alpha (12/13) signalling events.
DR SignaLink; P35368; -.
DR SIGNOR; P35368; -.
DR BioGRID-ORCS; 147; 11 hits in 1078 CRISPR screens.
DR ChiTaRS; ADRA1B; human.
DR GeneWiki; Alpha-1B_adrenergic_receptor; -.
DR GenomeRNAi; 147; -.
DR Pharos; P35368; Tclin.
DR PRO; PR:P35368; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; P35368; protein.
DR Bgee; ENSG00000170214; Expressed in right lobe of liver and 93 other tissues.
DR ExpressionAtlas; P35368; baseline and differential.
DR Genevisible; P35368; HS.
DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0004937; F:alpha1-adrenergic receptor activity; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
DR GO; GO:0150099; P:neuron-glial cell signaling; ISS:ARUK-UCL.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0001996; P:positive regulation of heart rate by epinephrine-norepinephrine; IBA:GO_Central.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IDA:UniProtKB.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; IBA:GO_Central.
DR GO; GO:0055117; P:regulation of cardiac muscle contraction; IEA:InterPro.
DR InterPro; IPR002233; ADR_fam.
DR InterPro; IPR001115; ADRA1B_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR PANTHER; PTHR24248:SF17; PTHR24248:SF17; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01103; ADRENERGICR.
DR PRINTS; PR00556; ADRENRGCA1BR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Lipoprotein; Membrane; Nucleus; Palmitate; Phosphoprotein;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..520
FT /note="Alpha-1B adrenergic receptor"
FT /id="PRO_0000069069"
FT TOPO_DOM 1..45
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 46..70
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 71..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 84..105
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 106..115
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 116..141
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 142..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 162..184
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 185..201
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 202..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 225..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 296..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 320..326
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 327..351
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 352..520
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 394..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 479..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 368..380
FT /note="Nuclear localization signal"
FT COMPBIAS 407..425
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 264
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P97717"
FT LIPID 365
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 118..195
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 51
FT /note="V -> G (in dbSNP:rs8192448)"
FT /id="VAR_019510"
FT MUTAGEN 368..380
FT /note="RGRGRRRRRRRRR->AGAGAAAAAAAAA: Abolishes targeting to
FT the nuclear membrane of cardiac myocytes."
FT /evidence="ECO:0000269|PubMed:22120526"
FT CONFLICT 371
FT /note="Missing (in Ref. 1 and 3; AAB59485)"
FT /evidence="ECO:0000305"
FT CONFLICT 498..501
FT /note="AAAD -> PRH (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 520 AA; 56836 MW; F081A23D2F943F7F CRC64;
MNPDLDTGHN TSAPAHWGEL KNANFTGPNQ TSSNSTLPQL DITRAISVGL VLGAFILFAI
VGNILVILSV ACNRHLRTPT NYFIVNLAMA DLLLSFTVLP FSAALEVLGY WVLGRIFCDI
WAAVDVLCCT ASILSLCAIS IDRYIGVRYS LQYPTLVTRR KAILALLSVW VLSTVISIGP
LLGWKEPAPN DDKECGVTEE PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG
VMKEMSNSKE LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV
GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC SSKEFKRAFV
RILGCQCRGR GRRRRRRRRR LGGCAYTYRP WTRGGSLERS QSRKDSLDDS GSCLSGSQRT
LPSASPSPGY LGRGAPPPVE LCAFPEWKAP GALLSLPAPE PPGRRGRHDS GPLFTFKLLT
EPESPGTDGG ASNGGCEAAA DVANGQPGFK SNMPLAPGQF
