ID   DF322_SOLTU             Reviewed;          74 AA.
AC   P20346;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Defensin-like protein P322;
DE   AltName: Full=Probable protease inhibitor P322;
DE   Flags: Precursor;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bintje;
RX   AGRICOLA=IND92000050; DOI=10.1007/BF00027383;
RA   Stiekema W.J., Heidekamp F., Dirkse W.G., van Beckum J., de Haan P.,
RA   ten Bosch C., Louwerse J.D.;
RT   "Molecular cloning and analysis of four potato tuber mRNAs.";
RL   Plant Mol. Biol. 11:255-269(1988).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Tuber.
CC   -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC       (RTI/MTI-2) subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought (Ref.1) to be a protease inhibitor.
CC       {ECO:0000305}.
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DR   EMBL; X13180; CAA31577.1; -; mRNA.
DR   PIR; S05594; S05594.
DR   AlphaFoldDB; P20346; -.
DR   SMR; P20346; -.
DR   STRING; 4113.PGSC0003DMT400039131; -.
DR   eggNOG; ENOG502S7HM; Eukaryota.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P20346; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..74
FT                   /note="Defensin-like protein P322"
FT                   /id="PRO_0000007055"
FT   DISULFID        30..74
FT                   /evidence="ECO:0000250"
FT   DISULFID        41..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..68
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..70
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8414 MW;  FAFE1A87038F4B5A CRC64;
     MRFFATFFLL AMLVVATKMG PMRIAEARHC ESLSHRFKGP CTRDSNCASV CETERFSGGN
     CHGFRRRCFC TKPC