DFA20_MOUSE
ID DFA20_MOUSE Reviewed; 95 AA.
AC Q45VN2; Q8BH68;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Alpha-defensin 20;
DE AltName: Full=Cryptdin-4;
DE AltName: Full=Defensin-related cryptdin-20;
DE Flags: Precursor;
GN Name=Defa20; Synonyms=Defcr20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Kunming;
RA Chen B., Zha Y., Xu X., Deng X.;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: May have microbicidal activities. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ103703; AAZ17407.1; -; mRNA.
DR EMBL; AK008107; BAC25200.1; -; mRNA.
DR EMBL; AK008275; BAC25212.1; -; mRNA.
DR EMBL; AC239604; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40273.1; -.
DR RefSeq; NP_001170992.1; NM_001177521.1.
DR RefSeq; NP_899091.2; NM_183268.4.
DR AlphaFoldDB; Q45VN2; -.
DR PaxDb; Q45VN2; -.
DR PeptideAtlas; Q45VN2; -.
DR PRIDE; Q45VN2; -.
DR DNASU; 68009; -.
DR Ensembl; ENSMUST00000084041; ENSMUSP00000081054; ENSMUSG00000094818.
DR Ensembl; ENSMUST00000084042; ENSMUSP00000081055; ENSMUSG00000095066.
DR GeneID; 100041890; -.
DR GeneID; 68009; -.
DR KEGG; mmu:100041890; -.
DR KEGG; mmu:68009; -.
DR UCSC; uc009lbq.2; mouse.
DR CTD; 100041890; -.
DR CTD; 68009; -.
DR MGI; MGI:1915259; Defa20.
DR VEuPathDB; HostDB:ENSMUSG00000094818; -.
DR VEuPathDB; HostDB:ENSMUSG00000095066; -.
DR GeneTree; ENSGT00940000153268; -.
DR HOGENOM; CLU_160803_1_0_1; -.
DR InParanoid; Q45VN2; -.
DR OMA; QARIVCH; -.
DR OrthoDB; 1610714at2759; -.
DR PhylomeDB; Q45VN2; -.
DR TreeFam; TF338414; -.
DR Reactome; R-MMU-1461973; Defensins.
DR Reactome; R-MMU-1462054; Alpha-defensins.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 100041890; 1 hit in 23 CRISPR screens.
DR BioGRID-ORCS; 68009; 1 hit in 29 CRISPR screens.
DR ChiTaRS; Defa20; mouse.
DR PRO; PR:Q45VN2; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q45VN2; protein.
DR Bgee; ENSMUSG00000094818; Expressed in ileum and 16 other tissues.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IDA:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IBA:GO_Central.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0051873; P:killing by host of symbiont cells; IDA:CACAO.
DR GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
PE 3: Inferred from homology;
KW Antibiotic; Antimicrobial; Defensin; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..58
FT /evidence="ECO:0000250"
FT /id="PRO_0000300066"
FT PEPTIDE 59..95
FT /note="Alpha-defensin 20"
FT /id="PRO_0000300067"
FT REGION 22..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 64..89
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..88
FT /evidence="ECO:0000250"
FT CONFLICT 87
FT /note="F -> Y (in Ref. 1; AAZ17407)"
FT /evidence="ECO:0000305"
FT CONFLICT 90..95
FT /note="RRRHRH -> PRR (in Ref. 1; AAZ17407)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 95 AA; 10601 MW; 46724F6A336EF7C1 CRC64;
MKTLVLLSAL VLLAFQVQAD PIQNTDEETN TEEQPGEEDQ AVSVSFGDPE GSALHEKSSR
DLICYCRKGG CNRGEQVYGT CSGRLLFCCR RRHRH
