ID   DFA4_NOSS1              Reviewed;         575 AA.
AC   Q8YNW7;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Putative diflavin flavoprotein A 4;
DE            EC=1.-.-.-;
GN   Name=dfa4; OrderedLocusNames=all4444;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- FUNCTION: Mediates electron transfer from NADH to oxygen, reducing it
CC       to water. This modular protein has 3 redox cofactors, in other
CC       organisms the same activity requires 2 or 3 proteins (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC       Note=Binds 2 iron ions per subunit.;
CC   -!- MISCELLANEOUS: By homology with NorV in E.coli, may be involved in
CC       nitric oxide detoxification. {ECO:0000250}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the zinc metallo-
CC       hydrolase group 3 family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the flavodoxin
CC       reductase family. {ECO:0000305}.
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DR   EMBL; BA000019; BAB76143.1; -; Genomic_DNA.
DR   PIR; AD2361; AD2361.
DR   RefSeq; WP_010998577.1; NZ_RSCN01000054.1.
DR   AlphaFoldDB; Q8YNW7; -.
DR   SMR; Q8YNW7; -.
DR   STRING; 103690.17133580; -.
DR   EnsemblBacteria; BAB76143; BAB76143; BAB76143.
DR   KEGG; ana:all4444; -.
DR   eggNOG; COG0426; Bacteria.
DR   eggNOG; COG1853; Bacteria.
DR   OMA; PEAPIYC; -.
DR   OrthoDB; 1149616at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016646; F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor; IEA:UniProt.
DR   Gene3D; 2.30.110.10; -; 1.
DR   Gene3D; 3.40.50.360; -; 1.
DR   Gene3D; 3.60.15.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR008254; Flavodoxin/NO_synth.
DR   InterPro; IPR001226; Flavodoxin_CS.
DR   InterPro; IPR029039; Flavoprotein-like_sf.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   Pfam; PF00258; Flavodoxin_1; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
DR   SMART; SM00849; Lactamase_B; 1.
DR   SUPFAM; SSF52218; SSF52218; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
DR   PROSITE; PS00201; FLAVODOXIN; 1.
DR   PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE   3: Inferred from homology;
KW   Electron transport; Oxidoreductase; Reference proteome; Transport.
FT   CHAIN           1..575
FT                   /note="Putative diflavin flavoprotein A 4"
FT                   /id="PRO_0000216796"
FT   DOMAIN          263..405
FT                   /note="Flavodoxin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT   REGION          41..234
FT                   /note="Zinc metallo-hydrolase"
FT   REGION          429..575
FT                   /note="Flavodoxin-reductase-like"
SQ   SEQUENCE   575 AA;  63749 MW;  B94428B16882FB70 CRC64;
     MTIATSSRPR DVQVADIGEH TLILRSRTWE RLKFEVEYSR QRGTTANSYL IQADKKALID
     PPGESFTAIY LEQLAQYLDF TTLDYIILGH VNPNRRVTLQ ELLSKAPQAT LICSRPAANA
     LKTAFPEWES RIQAVRFEDI LDLGQGHQLT FVTAPTPRWP DGLFTYDSAT KILYTDKFFG
     AHICEDTLFD EDWKKLDAER HYYFDCLHAP QAKQVEAALD KVVMLGARSY APGHGPVVRY
     SLSRFTYDYR QWCQGQKSQD LNVALLYTSA YGNTGILANA IAQGLVQNDV NVQSVNCELA
     DTAEITRIVE ACDGIIIGSP TLGGHAPTQI QTALGIVLST AAKTKLAGVF GSYGWSGEAI
     DLIESKLKDA NYRLGFDTIR VRFSPTPEIL QQCQAAGATF AQTLKKNKKL RTPRQVIPEA
     KIDRTEQAVG RIIGSLCVVT TRDQESHKGI LTSWVSQATF NPPGIMMAIA QEQNADLMSH
     TGDQFVLNIL KEGRNVRRYF SRQSTLGDNP FANLKTKTAD NGCLILTEAL AYLECTVTNQ
     LECGDRLLIY AVVDKGEVLA NDGVTAVEHR KSGSH