DFO_ARATH
ID DFO_ARATH Reviewed; 233 AA.
AC Q8RX33; Q9LMJ9;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein DOUBLE-STRAND BREAK FORMATION {ECO:0000303|PubMed:22694475};
DE Short=AtDFO {ECO:0000303|PubMed:22694475};
DE Short=Protein DSB FORMATION {ECO:0000303|PubMed:22694475};
GN Name=DFO {ECO:0000303|PubMed:22694475};
GN OrderedLocusNames=At1g07060 {ECO:0000312|Araport:AT1G07060};
GN ORFNames=F10K1.23 {ECO:0000312|EMBL:AAF82214.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=22694475; DOI=10.1111/j.1365-313x.2012.05075.x;
RA Zhang C., Song Y., Cheng Z.-H., Wang Y.-X., Zhu J., Ma H., Xu L.,
RA Yang Z.-N.;
RT "The Arabidopsis thaliana DSB formation (AtDFO) gene is required for
RT meiotic double-strand break formation.";
RL Plant J. 72:271-281(2012).
RN [5]
RP INTERACTION WITH PRD1.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=28855712; DOI=10.1038/s41598-017-10270-9;
RA Tang Y., Yin Z., Zeng Y., Zhang Q., Chen L., He Y., Lu P., Ye D., Zhang X.;
RT "MTOPVIB interacts with AtPRD1 and plays important roles in formation of
RT meiotic DNA double-strand breaks in Arabidopsis.";
RL Sci. Rep. 7:10007-10007(2017).
CC -!- FUNCTION: Required for meiotic double-strand break (DSB) formation, the
CC initial event for meiotic recombination. {ECO:0000269|PubMed:22694475}.
CC -!- SUBUNIT: Interacts with PRD1; this interaction facilitates a binding to
CC PRD3. {ECO:0000269|PubMed:28855712}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RX33-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RX33-2; Sequence=VSP_060397;
CC -!- TISSUE SPECIFICITY: Specifically expressed in buds.
CC {ECO:0000269|PubMed:22694475}.
CC -!- DEVELOPMENTAL STAGE: In flowers, first detected at the early anther
CC development stages with stronger expression at stages 6 and 7
CC (PubMed:22694475). Also observed during ovule development with high
CC levels during meiosis (PubMed:22694475). {ECO:0000269|PubMed:22694475}.
CC -!- DISRUPTION PHENOTYPE: Reduced fertility upon self-pollination,
CC producing polyads with an abnormal number of microspores during pollen
CC formation (PubMed:22694475). Meiocytes are defective in homologous
CC chromosome synapsis and segregation (PubMed:22694475). Homologous
CC recombination is severely affected during meiotic prophase I
CC (PubMed:22694475). {ECO:0000269|PubMed:22694475}.
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DR EMBL; AC067971; AAF82214.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28073.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60224.1; -; Genomic_DNA.
DR EMBL; AY090924; AAM13919.1; -; mRNA.
DR PIR; D86205; D86205.
DR RefSeq; NP_001322524.1; NM_001331678.1. [Q8RX33-2]
DR RefSeq; NP_172187.1; NM_100580.2. [Q8RX33-1]
DR AlphaFoldDB; Q8RX33; -.
DR STRING; 3702.AT1G07060.1; -.
DR PaxDb; Q8RX33; -.
DR PRIDE; Q8RX33; -.
DR DNASU; 837217; -.
DR EnsemblPlants; AT1G07060.1; AT1G07060.1; AT1G07060. [Q8RX33-1]
DR EnsemblPlants; AT1G07060.2; AT1G07060.2; AT1G07060. [Q8RX33-2]
DR GeneID; 837217; -.
DR Gramene; AT1G07060.1; AT1G07060.1; AT1G07060. [Q8RX33-1]
DR Gramene; AT1G07060.2; AT1G07060.2; AT1G07060. [Q8RX33-2]
DR KEGG; ath:AT1G07060; -.
DR Araport; AT1G07060; -.
DR TAIR; locus:2007442; AT1G07060.
DR eggNOG; ENOG502RXSP; Eukaryota.
DR HOGENOM; CLU_098110_0_0_1; -.
DR InParanoid; Q8RX33; -.
DR OrthoDB; 1341529at2759; -.
DR PhylomeDB; Q8RX33; -.
DR PRO; PR:Q8RX33; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RX33; baseline and differential.
DR GO; GO:0051321; P:meiotic cell cycle; IGI:TAIR.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IGI:TAIR.
DR InterPro; IPR044969; DFO.
DR PANTHER; PTHR37176; PTHR37176; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Meiosis; Reference proteome.
FT CHAIN 1..233
FT /note="Protein DOUBLE-STRAND BREAK FORMATION"
FT /id="PRO_0000448440"
FT VAR_SEQ 212..233
FT /note="GIFIYIVSSLKFAVCNRLLTTF -> VQAQGAGYLRSKEHRIQSRQTLPTKN
FT PDCTGSNLFREGIKKRNERMLQDLRSIRMNRNLEPDVRCV (in isoform 2)"
FT /id="VSP_060397"
SQ SEQUENCE 233 AA; 26396 MW; F57DF2E04F2AD979 CRC64;
MRHNIKFKSK GTLKIRNTAQ ISLWKKCSDS MIADQTYLFI NRVQDRRFDE ESLRILELSL
VAMNVKSFLE VRSRLRDFMR SESVVIFGEL TGESMVAKLS VLEFFARAFA LLGDMESCLA
MRYEALNLRQ LKSPSCLWLG VSHSEWTKFA VQSMENGFPS IAGKASENAL LSLKKDSLIE
PKSEDNSDIL DAAEKVRRLR DSAASLTSSH SGIFIYIVSS LKFAVCNRLL TTF
