ID   DFRA_GERHY              Reviewed;         366 AA.
AC   P51105;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Dihydroflavonol 4-reductase;
DE            Short=DFR;
DE            EC=1.1.1.219 {ECO:0000250|UniProtKB:Q9XES5};
DE   AltName: Full=Dihydrokaempferol 4-reductase;
DE   AltName: Full=Flavanone 4-reductase;
DE            Short=FNR;
DE            EC=1.1.1.234 {ECO:0000250|UniProtKB:Q9XES5};
GN   Name=DFR;
OS   Gerbera hybrida (Daisy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Mutisioideae; Mutisieae;
OC   Gerbera.
OX   NCBI_TaxID=18101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Regina; TISSUE=Corolla;
RX   PubMed=8507822; DOI=10.1007/bf00014927;
RA   Helariutta Y., Elomaa P., Kotilainen M., Seppanen P., Teeri T.H.;
RT   "Cloning of cDNA coding for dihydroflavonol-4-reductase (DFR) and
RT   characterization of dfr expression in the corollas of Gerbera hybrida var.
RT   Regina (Compositae).";
RL   Plant Mol. Biol. 22:183-193(1993).
CC   -!- FUNCTION: Bifunctional enzyme involved in flavonoid metabolism.
CC       {ECO:0000250|UniProtKB:Q9XES5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-
CC         dihydroflavonol + H(+) + NADPH; Xref=Rhea:RHEA:54444,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:138176, ChEBI:CHEBI:138188; EC=1.1.1.219;
CC         Evidence={ECO:0000250|UniProtKB:Q9XES5};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + H(+) + NADPH;
CC         Xref=Rhea:RHEA:11228, ChEBI:CHEBI:15378, ChEBI:CHEBI:15605,
CC         ChEBI:CHEBI:15606, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.234; Evidence={ECO:0000250|UniProtKB:Q9XES5};
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. Dihydroflavonol-4-reductase subfamily. {ECO:0000305}.
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DR   EMBL; Z17221; CAA78930.1; -; mRNA.
DR   PIR; S35189; S35189.
DR   AlphaFoldDB; P51105; -.
DR   SMR; P51105; -.
DR   PRIDE; P51105; -.
DR   BRENDA; 1.1.1.219; 2420.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0045552; F:dihydrokaempferol 4-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047890; F:flavanone 4-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; NADP; Oxidoreductase.
FT   CHAIN           1..366
FT                   /note="Dihydroflavonol 4-reductase"
FT                   /id="PRO_0000215566"
FT   BINDING         45
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0A059TC02"
FT   BINDING         164
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:A0A059TC02"
SQ   SEQUENCE   366 AA;  41102 MW;  8B12719BD0D73165 CRC64;
     MEEDSPATVC VTGAAGFIGS WLVMRLLERG YVVHATVRDP GDLKKVKHLL ELPKAQTNLK
     LWKADLTQEG SFDEAIQGCH GVFHLATPMD FESKDPENEI IKPTIEGVLS IIRSCVKAKT
     VKKLVFTSSA GTVNGQEKQL HVYDESHWSD LDFIYSKKMT AWMYFVSKTL AEKAAWDATK
     GNNISFISII PTLVVGPFIT STFPPSLVTA LSLITGNEAH YSIIKQGQYV HLDDLCECHI
     YLYENPKAKG RYICSSHDAT IHQLAKIIKD KWPEYYIPTK FPGIDEELPI VSFSSKKLID
     TGFEFKYNLE DMFKGAIDTC REKGLLPYST IKNHINGNHV NGVHHYIKNN DDDHEKGLLC
     CSKEGQ