DGAL_HAEIN
ID DGAL_HAEIN Reviewed; 331 AA.
AC P44883;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=D-galactose-binding periplasmic protein;
DE Short=GBP;
DE AltName: Full=D-galactose/ D-glucose-binding protein;
DE Short=GGBP;
DE Flags: Precursor;
GN Name=mglB; OrderedLocusNames=HI_0822;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP PROTEIN SEQUENCE OF 25-29.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=10675023;
RX DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA Fountoulakis M.;
RT "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL Electrophoresis 21:411-429(2000).
CC -!- FUNCTION: This protein is involved in the active transport of galactose
CC and glucose. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- DOMAIN: The calcium-binding site is structurally similar to that of EF-
CC hand proteins, but is in two parts, with the last calcium ligand
CC provided by Glu-229. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC22481.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L42023; AAC22481.1; ALT_INIT; Genomic_DNA.
DR PIR; G64096; G64096.
DR RefSeq; NP_438982.2; NC_000907.1.
DR RefSeq; WP_010869062.1; NC_000907.1.
DR AlphaFoldDB; P44883; -.
DR SMR; P44883; -.
DR STRING; 71421.HI_0822; -.
DR EnsemblBacteria; AAC22481; AAC22481; HI_0822.
DR KEGG; hin:HI_0822; -.
DR PATRIC; fig|71421.8.peg.863; -.
DR eggNOG; COG1879; Bacteria.
DR HOGENOM; CLU_037628_3_1_6; -.
DR PhylomeDB; P44883; -.
DR BioCyc; HINF71421:G1GJ1-863-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR CDD; cd01539; PBP1_GGBP; 1.
DR InterPro; IPR044085; MglB-like_PBP1.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR025997; SBP_2_dom.
DR Pfam; PF13407; Peripla_BP_4; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Metal-binding; Periplasm;
KW Reference proteome; Signal; Sugar transport; Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:10675023"
FT CHAIN 25..331
FT /note="D-galactose-binding periplasmic protein"
FT /id="PRO_0000031724"
FT BINDING 38
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 38
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 115
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 115
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 158
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 160
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 162
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 164
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 166
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 176
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 176
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 178
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 178
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 182
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 182
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 229
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 235
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 235
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 259
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 259
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
FT BINDING 279
FT /ligand="beta-D-galactose"
FT /ligand_id="ChEBI:CHEBI:27667"
FT /evidence="ECO:0000250|UniProtKB:P23905"
FT BINDING 279
FT /ligand="beta-D-glucose"
FT /ligand_id="ChEBI:CHEBI:15903"
FT /evidence="ECO:0000250|UniProtKB:P0AEE5"
SQ SEQUENCE 331 AA; 35518 MW; D4A0E639D9FD22A2 CRC64;
MKKTAVLSTV AFAIALGSAS ASFAADNRIG VTIYKYDDNF MSLMRKEIDK EAKVVGGIKL
LMNDSQNAQS IQNDQVDILL SKGVKALAIN LVDPAAAPTI IGKAKSDNIP VVFFNKDPGA
KAIGSYEQAY YVGTDPKESG LIQGDLIAKQ WKANPALDLN KDGKIQFVLL KGEPGHPDAE
VRTKYVIEEL NAKGIQTEQL FIDTGMWDAA MAKDKVDAWL SSSKANDIEV IISNNDGMAL
GALEATKAHG KKLPIFGVDA LPEALQLISK GELAGTVLND SVNQGKAVVQ LSNNLAQGKS
ATEGTKWELK DRVVRIPYVG VDKDNLGDFL K
