DGAT2_RICCO
ID DGAT2_RICCO Reviewed; 340 AA.
AC A1A442; A7YJ37;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Diacylglycerol O-acyltransferase 2 {ECO:0000305};
DE Short=RcDGAT2 {ECO:0000303|PubMed:17084870};
DE EC=2.3.1.20 {ECO:0000269|PubMed:17084870};
GN Name=DGAT2 {ECO:0000303|PubMed:17084870};
GN ORFNames=RCOM_0613570 {ECO:0000312|EMBL:EEF33843.1};
OS Ricinus communis (Castor bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC Ricinus.
OX NCBI_TaxID=3988;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=17084870; DOI=10.1016/j.phytochem.2006.09.020;
RA Kroon J.T., Wei W., Simon W.J., Slabas A.R.;
RT "Identification and functional expression of a type 2 acyl-
RT CoA:diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds
RT which has high homology to the major triglyceride biosynthetic enzyme of
RT fungi and animals.";
RL Phytochemistry 67:2541-2549(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Burgal J., Shockey J., Lu C., Dyer J., Larson T., Graham I., Browse J.;
RT "Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2
RT drives dramatic increases in ricinoleate levels in seed oil.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Lin J.-H., McKeon T.;
RT "Isolation and sequence analysis of a novel cDNA encoding a putative
RT diacylglycerol acyltransferase (DGAT2) from Ricinus communis (castor
RT bean).";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Hale;
RX PubMed=20729833; DOI=10.1038/nbt.1674;
RA Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA Rabinowicz P.D.;
RT "Draft genome sequence of the oilseed species Ricinus communis.";
RL Nat. Biotechnol. 28:951-956(2010).
CC -!- FUNCTION: Involved in triacylglycerol (TAG) synthesis
CC (PubMed:17084870). Catalyzes the acylation of the sn-3 hydroxy group of
CC sn-1,2-diricinolein using ricinoleoyl-CoA (PubMed:17084870).
CC {ECO:0000269|PubMed:17084870}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000269|PubMed:17084870};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10869;
CC Evidence={ECO:0000269|PubMed:17084870};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(12R)-hydroxy-(9Z)-octadecenoyl-CoA + 1,2-di-(12R-hydroxy-9Z-
CC octadecenoyl)-sn-glycerol = 1,2,3-tri-(12R-hydroxy-9Z-octadecenoyl)-
CC glycerol + CoA; Xref=Rhea:RHEA:56536, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:139559, ChEBI:CHEBI:140471, ChEBI:CHEBI:140525;
CC Evidence={ECO:0000269|PubMed:17084870};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:56537;
CC Evidence={ECO:0000269|PubMed:17084870};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9ASU1}; Multi-pass membrane protein
CC {ECO:0000255}. Lipid droplet {ECO:0000250|UniProtKB:Q9ASU1}.
CC -!- DEVELOPMENTAL STAGE: Expressed in endosperm of developing seeds from 10
CC to 30 days after pollination, with a peak at 25 days.
CC {ECO:0000269|PubMed:17084870}.
CC -!- SIMILARITY: Belongs to the diacylglycerol acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; DQ923084; ABI83668.1; -; mRNA.
DR EMBL; EU391592; ACB30544.1; -; mRNA.
DR EMBL; AY916129; AAY16324.1; -; mRNA.
DR EMBL; EQ974096; EEF33843.1; -; Genomic_DNA.
DR RefSeq; NP_001310616.1; NM_001323687.1.
DR AlphaFoldDB; A1A442; -.
DR STRING; 3988.XP_002528531.1; -.
DR SwissLipids; SLP:000001918; -.
DR GeneID; 8258757; -.
DR KEGG; rcu:8258757; -.
DR eggNOG; KOG0831; Eukaryota.
DR InParanoid; A1A442; -.
DR OrthoDB; 1347007at2759; -.
DR BRENDA; 2.3.1.20; 1204.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000008311; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IDA:UniProtKB.
DR GO; GO:0006629; P:lipid metabolic process; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IDA:UniProtKB.
DR InterPro; IPR007130; DAGAT.
DR Pfam; PF03982; DAGAT; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Endoplasmic reticulum; Lipid biosynthesis; Lipid droplet;
KW Lipid metabolism; Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..340
FT /note="Diacylglycerol O-acyltransferase 2"
FT /id="PRO_0000449829"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 234
FT /note="G -> V (in Ref. 1; ABI83668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 38662 MW; A09FFC7A3E130B95 CRC64;
MGEEANHNNN NNNINSNDEK NEEKSNYTVV NSRELYPTNI FHALLALSIW IGSIHFNLFL
LFISYLFLSF PTFLLIVGFF VVLMFIPIDE HSKLGRRLCR YVCRHACSHF PVTLHVEDMN
AFHSDRAYVF GYEPHSVFPL GVSVLSDHFA VLPLPKMKVL ASNAVFRTPV LRHIWTWCGL
TSATKKNFTA LLASGYSCIV IPGGVQETFY MKHGSEIAFL KARRGFVRVA MEMGKPLVPV
FCFGQSNVYK WWKPDGELFM KIARAIKFSP IVFWGVLGSH LPLQRPMHVV VGKPIEVKQN
PQPTVEEVSE VQGQFVAALK DLFERHKARV GYADLTLEIL
