ID   3CP2_STRSQ              Reviewed;          21 AA.
AC   P85078;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 2.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Tricyclic peptide MS-271 {ECO:0000303|PubMed:8689231, ECO:0000303|PubMed:8689232};
DE   AltName: Full=Class I lasso peptide {ECO:0000305};
OS   Streptomyces sp.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1931;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, D-AMINO ACID AT TRP-21,
RP   DISULFIDE BONDS, AND CROSS-LINK.
RC   STRAIN=M-271 {ECO:0000269|PubMed:8689231};
RX   PubMed=8689231; DOI=10.1016/0968-0896(95)00175-1;
RA   Yano K., Toki S., Nakanishi S., Ochiai K., Ando K., Yoshida M., Matsuda Y.,
RA   Yamasaki M.;
RT   "MS-271, a novel inhibitor of calmodulin-activated myosin light chain
RT   kinase from Streptomyces sp. -- I. Isolation, structural determination and
RT   biological properties of MS-271.";
RL   Bioorg. Med. Chem. 4:115-120(1996).
RN   [2] {ECO:0000305}
RP   STRUCTURE BY NMR OF 1-21.
RX   PubMed=8689232; DOI=10.1016/0968-0896(95)00176-x;
RA   Katahira R., Yamasaki M., Matsuda Y., Yoshida M.;
RT   "MS-271, a novel inhibitor of calmodulin-activated myosin light chain
RT   kinase from Streptomyces sp. -- II. Solution structure of MS-271:
RT   characteristic features of the 'lasso' structure.";
RL   Bioorg. Med. Chem. 4:121-129(1996).
CC   -!- FUNCTION: Inhibits chicken myosin light chain kinase with an IC(50) of
CC       8 M. Does not inhibit bovine cAMP-dependent protein kinase or rat
CC       protein kinase C. Antibacterial activity against the Gram-positive
CC       bacteria B.subtilis, E.faecium and S.aureus. No antibacterial activity
CC       against the Gram-negative bacteria E.coli, K.pneumoniae, P.aeruginosa,
CC       P.vulgaris, S.sonnei and S.typhosa. No antifungal activity against
CC       C.albicans. {ECO:0000269|PubMed:8689231}.
CC   -!- MASS SPECTROMETRY: Mass=2168.8596; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:8689231};
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DR   AlphaFoldDB; P85078; -.
DR   GO; GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0006469; P:negative regulation of protein kinase activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; D-amino acid; Direct protein sequencing;
KW   Disulfide bond; Protein kinase inhibitor; Thioether bond.
FT   PEPTIDE         1..21
FT                   /note="Tricyclic peptide MS-271"
FT                   /evidence="ECO:0000269|PubMed:8689231"
FT                   /id="PRO_0000278145"
FT   MOD_RES         21
FT                   /note="D-tryptophan"
FT                   /evidence="ECO:0000269|PubMed:8689231"
FT   DISULFID        1..13
FT                   /evidence="ECO:0000269|PubMed:8689231"
FT   DISULFID        7..19
FT                   /evidence="ECO:0000269|PubMed:8689231"
FT   CROSSLNK        1..9
FT                   /note="3-cysteinyl-aspartic acid (Cys-Asp)"
FT                   /evidence="ECO:0000269|PubMed:8689231"
SQ   SEQUENCE   21 AA;  2186 MW;  EF82967204A4FF91 CRC64;
     CLGVGSCNDF AGCGYAIVCF W