ID   DGK1_ORYSJ              Reviewed;         499 AA.
AC   Q7XQT2; A0A0P0WF54;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Diacylglycerol kinase 1 {ECO:0000303|PubMed:30887532};
DE            Short=DAG kinase 1 {ECO:0000305};
DE            Short=OsDGK1 {ECO:0000303|PubMed:30887532};
DE            EC=2.7.1.107 {ECO:0000269|PubMed:30887532};
DE   AltName: Full=BTH-inducible diacylglycerol kinase 1 {ECO:0000303|PubMed:18679055};
DE            Short=OsBIDK1 {ECO:0000303|PubMed:18679055};
GN   Name=DGK1 {ECO:0000303|PubMed:30887532};
GN   Synonyms=BIDK1 {ECO:0000303|PubMed:18679055};
GN   OrderedLocusNames=Os04g0634700 {ECO:0000312|EMBL:BAS91203.1},
GN   LOC_Os04g54200 {ECO:0000305};
GN   ORFNames=OSJNBa0043L09.15 {ECO:0000312|EMBL:BAS91203.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=18679055;
RA   Zhang W., Chen J., Zhang H., Song F.;
RT   "Overexpression of a rice diacylglycerol kinase gene OsBIDK1 enhances
RT   disease resistance in transgenic tobacco.";
RL   Mol. Cells 26:258-264(2008).
RN   [7]
RP   FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=30887532; DOI=10.1111/nph.15801;
RA   Yuan S., Kim S.C., Deng X., Hong Y., Wang X.;
RT   "Diacylglycerol kinase and associated lipid mediators modulate rice root
RT   architecture.";
RL   New Phytol. 223:261-276(2019).
CC   -!- FUNCTION: Phosphorylates the second messenger diacylglycerol (DAG) to
CC       generate phosphatidic acid (PA), another important signaling molecule
CC       (PubMed:30887532). PA is required for plant development and responses
CC       to abiotic stress (Probable). May play a role in disease resistance
CC       responses to pathogen attack (Probable). Modulates root architecture by
CC       regulating the ratio of DAG and PA, which have opposite effect on the
CC       promotion or suppression of lateral roots vs seminal roots
CC       (PubMed:30887532). Suppresses lateral root number, but promotes seminal
CC       root and crown root thickness (PubMed:30887532).
CC       {ECO:0000269|PubMed:30887532, ECO:0000305|PubMed:18679055}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-
CC         phosphate + ADP + H(+); Xref=Rhea:RHEA:10272, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17815, ChEBI:CHEBI:30616, ChEBI:CHEBI:58608,
CC         ChEBI:CHEBI:456216; EC=2.7.1.107;
CC         Evidence={ECO:0000269|PubMed:30887532};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10273;
CC         Evidence={ECO:0000269|PubMed:30887532};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P23743}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in roots.
CC       {ECO:0000269|PubMed:30887532}.
CC   -!- INDUCTION: Induced by infection with the rice blast fungus Magnaporthe
CC       oryzae, and the inducer of plant defense responses benzothiadiazole
CC       (BTH). {ECO:0000269|PubMed:18679055}.
CC   -!- DISRUPTION PHENOTYPE: Increased number and density of lateral roots and
CC       decreased thickness of seminal roots. {ECO:0000269|PubMed:30887532}.
CC   -!- MISCELLANEOUS: Plants overexpressing DGK1 exhibit decreased density of
CC       lateral roots and increased thickness of seminal roots.
CC       {ECO:0000269|PubMed:30887532}.
CC   -!- SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family.
CC       {ECO:0000305}.
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DR   EMBL; AL606444; CAE02996.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF15915.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS91203.1; -; Genomic_DNA.
DR   EMBL; AK100906; BAG94821.1; -; mRNA.
DR   RefSeq; XP_015633855.1; XM_015778369.1.
DR   AlphaFoldDB; Q7XQT2; -.
DR   STRING; 4530.OS04T0634700-01; -.
DR   PaxDb; Q7XQT2; -.
DR   PRIDE; Q7XQT2; -.
DR   EnsemblPlants; Os04t0634700-01; Os04t0634700-01; Os04g0634700.
DR   GeneID; 4337126; -.
DR   Gramene; Os04t0634700-01; Os04t0634700-01; Os04g0634700.
DR   eggNOG; KOG1169; Eukaryota.
DR   HOGENOM; CLU_002706_46_2_1; -.
DR   InParanoid; Q7XQT2; -.
DR   OMA; INQSTYA; -.
DR   OrthoDB; 633642at2759; -.
DR   BRENDA; 2.7.1.107; 8948.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004143; F:diacylglycerol kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003951; F:NAD+ kinase activity; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; IEA:InterPro.
DR   Gene3D; 3.40.50.10330; -; 1.
DR   InterPro; IPR017438; ATP-NAD_kinase_N.
DR   InterPro; IPR037607; DGK.
DR   InterPro; IPR000756; Diacylglycerol_kin_accessory.
DR   InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR   InterPro; IPR016961; Diacylglycerol_kinase_pln.
DR   InterPro; IPR016064; NAD/diacylglycerol_kinase_sf.
DR   PANTHER; PTHR11255; PTHR11255; 1.
DR   Pfam; PF00609; DAGK_acc; 1.
DR   Pfam; PF00781; DAGK_cat; 1.
DR   PIRSF; PIRSF030829; Diacylglycerol_kinase_pln; 1.
DR   SMART; SM00045; DAGKa; 1.
DR   SMART; SM00046; DAGKc; 1.
DR   SUPFAM; SSF111331; SSF111331; 1.
DR   PROSITE; PS50146; DAGK; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Kinase; Nucleotide-binding; Plant defense; Reference proteome;
KW   Stress response; Transferase.
FT   CHAIN           1..499
FT                   /note="Diacylglycerol kinase 1"
FT                   /id="PRO_0000451305"
FT   DOMAIN          41..194
FT                   /note="DAGKc"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00783"
FT   REGION          442..479
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        442..456
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   499 AA;  55662 MW;  4941365934D4E84F CRC64;
     MDGHTNGTNG SCSKPCEPLT DYCIPDYILN PDSEQVLVDQ APCCPVVVFI NSRSGGQLGS
     SLIKTYRELL NKAQVFDLSE EAPEKVLHRL YCNFEKLKSN GDPIAFQIQS NLRLIVAGGD
     GTASWLLGVV SDLKLSHPPP IATVPLGTGN NLPFSFGWGK KNPTTDQEAV KSFLGQVKKA
     REMNIDSWHI IMRMRAPQEG PCEPIAPLEL PHSLHAFHRV SGSDSLNMEG YHTYRGGFWN
     YFSMGMDAQV SYEFHSERKR NPEKFKNQLT NQSTYAKLGL KQGWFAASLT HPSSRNIAQL
     AKVRIMKRPG GQWEELKIPR SIRSIVCLNL PSFSGGLNPW GTPGTRKVQD RDLTAPFVDD
     GLIEVVGFRD AWHGLVLLAP NGHGTRLAQA HRIRFEFHKG AAEHTFMRID GEPWKQPLPK
     DDDTVVVEIS HLRQVTMLAS DPCKSKSVND PSSPMCCSNH DDDERNSLED EDEWEEGRKK
     FGAADTFKFP DEVDVAHLS