ID   DGK2_LACJO              Reviewed;         224 AA.
AC   Q74HC2; Q59484; Q5FHS9;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Deoxyguanosine kinase;
DE            Short=DGK;
DE            Short=DGUO kinase;
DE            EC=2.7.1.113;
DE   AltName: Full=Deoxynucleoside kinase complex I F-component;
GN   OrderedLocusNames=LJ_1825;
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088;
RX   PubMed=7896798; DOI=10.1074/jbc.270.12.6602;
RA   Ma G.T., Hong Y.S., Ives D.H.;
RT   "Cloning and expression of the heterodimeric deoxyguanosine
RT   kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26.";
RL   J. Biol. Chem. 270:6595-6601(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533;
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-18.
RC   STRAIN=ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088;
RX   PubMed=8172906; DOI=10.1021/bi00183a041;
RA   Ikeda S., Ma G.T., Ives D.H.;
RT   "Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26:
RT   functional assignment of subunits using limited proteolysis controlled by
RT   end-product inhibitors.";
RL   Biochemistry 33:5328-5334(1994).
CC   -!- FUNCTION: DGK/DAK plays an essential role in generating the
CC       deoxyribonucleotide precursors, dGTP and dATP, for DNA metabolism.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2'-deoxyguanosine + ATP = ADP + dGMP + H(+);
CC         Xref=Rhea:RHEA:19201, ChEBI:CHEBI:15378, ChEBI:CHEBI:17172,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:57673, ChEBI:CHEBI:456216;
CC         EC=2.7.1.113;
CC   -!- SUBUNIT: Heterodimer of a deoxyadenosine (DAK) and a deoxyguanosine
CC       kinase (DGK).
CC   -!- SIMILARITY: Belongs to the DCK/DGK family. {ECO:0000305}.
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DR   EMBL; U01881; AAB09751.1; -; Genomic_DNA.
DR   EMBL; AE017198; AAS09770.1; -; Genomic_DNA.
DR   PIR; B56168; B56168.
DR   RefSeq; WP_011162602.1; NC_005362.1.
DR   AlphaFoldDB; Q74HC2; -.
DR   SMR; Q74HC2; -.
DR   STRING; 257314.LJ_1825; -.
DR   EnsemblBacteria; AAS09770; AAS09770; LJ_1825.
DR   KEGG; ljo:LJ_1825; -.
DR   PATRIC; fig|257314.6.peg.1826; -.
DR   eggNOG; COG1428; Bacteria.
DR   HOGENOM; CLU_030466_2_1_9; -.
DR   OMA; IHVRVSF; -.
DR   BRENDA; 2.7.1.113; 2873.
DR   SABIO-RK; Q74HC2; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004138; F:deoxyguanosine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd01673; dNK; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR002624; DCK/DGK.
DR   InterPro; IPR031314; DNK_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01712; dNK; 1.
DR   PIRSF; PIRSF000705; DNK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Direct protein sequencing; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8172906"
FT   CHAIN           2..224
FT                   /note="Deoxyguanosine kinase"
FT                   /id="PRO_0000239237"
FT   ACT_SITE        78
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         8..16
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         32
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         44
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         84
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        173
FT                   /note="E -> A (in Ref. 1; AAB09751)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        214
FT                   /note="F -> L (in Ref. 1; AAB09751)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   224 AA;  26406 MW;  8DD02003CF396CDD CRC64;
     MTVIVLSGPI GAGKSSLTGI LSKYLGTNPF YESVDDNPVL PLFYENPKKY AFLLQVYFLN
     TRFRSIKSAL TDDNNVLDRS IYEDALFFQM NADIGRATPE EVDTYYELLH NMMSELDRMP
     KKNPDLLVHI DVSYDTMLKR IQKRGRNYEQ LSYDPTLEDY YKRLLRYYKP WYEKYDYSPK
     MTIDGDKLDF MASEEDRQEV LNQIVAKLKE MGKFEDDWKP NLVK