DGK5_ARATH
ID DGK5_ARATH Reviewed; 509 AA.
AC Q9C5E5; Q8LE70; Q9SKS9;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Diacylglycerol kinase 5;
DE Short=AtDGK5;
DE Short=DAG kinase 5;
DE EC=2.7.1.107;
DE AltName: Full=Diglyceride kinase 5;
DE Short=DGK 5;
GN Name=DGK5; OrderedLocusNames=At2g20900; ORFNames=F5H14.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Phosphorylates the second messenger diacylglycerol (DAG) to
CC generate phosphatidic acid (PA), another important signaling molecule.
CC PA is required for plant development and responses to abiotic stress
CC and pathogen attack. May be involved in the accumulation of PA during
CC cold stress (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-
CC phosphate + ADP + H(+); Xref=Rhea:RHEA:10272, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17815, ChEBI:CHEBI:30616, ChEBI:CHEBI:58608,
CC ChEBI:CHEBI:456216; EC=2.7.1.107;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C5E5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C5E5-2; Sequence=VSP_046410, VSP_046411;
CC -!- SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase family.
CC {ECO:0000305}.
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DR EMBL; AC006234; AAD20931.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07093.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07094.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07095.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07096.1; -; Genomic_DNA.
DR EMBL; AF360300; AAK26010.1; -; mRNA.
DR EMBL; BT000983; AAN41383.1; -; mRNA.
DR EMBL; AK316877; BAH19585.1; -; mRNA.
DR EMBL; AK317470; BAH20135.1; -; mRNA.
DR EMBL; AY085589; AAM62810.1; -; mRNA.
DR PIR; G84594; G84594.
DR RefSeq; NP_001031381.1; NM_001036304.1. [Q9C5E5-1]
DR RefSeq; NP_565492.1; NM_127660.4. [Q9C5E5-2]
DR RefSeq; NP_850007.1; NM_179676.3. [Q9C5E5-1]
DR RefSeq; NP_973498.1; NM_201769.3. [Q9C5E5-2]
DR AlphaFoldDB; Q9C5E5; -.
DR BioGRID; 1977; 1.
DR IntAct; Q9C5E5; 1.
DR STRING; 3702.AT2G20900.1; -.
DR iPTMnet; Q9C5E5; -.
DR PaxDb; Q9C5E5; -.
DR PRIDE; Q9C5E5; -.
DR ProteomicsDB; 224215; -. [Q9C5E5-1]
DR DNASU; 816624; -.
DR EnsemblPlants; AT2G20900.1; AT2G20900.1; AT2G20900. [Q9C5E5-1]
DR EnsemblPlants; AT2G20900.2; AT2G20900.2; AT2G20900. [Q9C5E5-2]
DR EnsemblPlants; AT2G20900.3; AT2G20900.3; AT2G20900. [Q9C5E5-2]
DR EnsemblPlants; AT2G20900.4; AT2G20900.4; AT2G20900. [Q9C5E5-1]
DR GeneID; 816624; -.
DR Gramene; AT2G20900.1; AT2G20900.1; AT2G20900. [Q9C5E5-1]
DR Gramene; AT2G20900.2; AT2G20900.2; AT2G20900. [Q9C5E5-2]
DR Gramene; AT2G20900.3; AT2G20900.3; AT2G20900. [Q9C5E5-2]
DR Gramene; AT2G20900.4; AT2G20900.4; AT2G20900. [Q9C5E5-1]
DR KEGG; ath:AT2G20900; -.
DR Araport; AT2G20900; -.
DR TAIR; locus:2051343; AT2G20900.
DR eggNOG; KOG1169; Eukaryota.
DR InParanoid; Q9C5E5; -.
DR OMA; DSWHIML; -.
DR OrthoDB; 633642at2759; -.
DR PhylomeDB; Q9C5E5; -.
DR BioCyc; ARA:AT2G20900-MON; -.
DR PRO; PR:Q9C5E5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9C5E5; baseline and differential.
DR Genevisible; Q9C5E5; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004143; F:diacylglycerol kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0003951; F:NAD+ kinase activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; IEA:InterPro.
DR Gene3D; 3.40.50.10330; -; 1.
DR InterPro; IPR017438; ATP-NAD_kinase_N.
DR InterPro; IPR037607; DGK.
DR InterPro; IPR000756; Diacylglycerol_kin_accessory.
DR InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR InterPro; IPR016961; Diacylglycerol_kinase_pln.
DR InterPro; IPR016064; NAD/diacylglycerol_kinase_sf.
DR PANTHER; PTHR11255; PTHR11255; 1.
DR Pfam; PF00609; DAGK_acc; 1.
DR Pfam; PF00781; DAGK_cat; 1.
DR PIRSF; PIRSF030829; Diacylglycerol_kinase_pln; 1.
DR SMART; SM00045; DAGKa; 1.
DR SMART; SM00046; DAGKc; 1.
DR SUPFAM; SSF111331; SSF111331; 1.
DR PROSITE; PS50146; DAGK; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Plant defense; Reference proteome; Stress response; Transferase.
FT CHAIN 1..509
FT /note="Diacylglycerol kinase 5"
FT /id="PRO_0000422113"
FT DOMAIN 36..187
FT /note="DAGKc"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00783"
FT REGION 439..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 465..491
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 485..491
FT /note="GEHSNKK -> VDISQLS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640, ECO:0000303|Ref.5"
FT /id="VSP_046410"
FT VAR_SEQ 492..509
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640, ECO:0000303|Ref.5"
FT /id="VSP_046411"
FT CONFLICT 31
FT /note="E -> Q (in Ref. 5; AAM62810)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="F -> S (in Ref. 5; AAM62810)"
FT /evidence="ECO:0000305"
FT CONFLICT 102
FT /note="R -> G (in Ref. 5; AAM62810)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 57377 MW; F6F9A08630D327DA CRC64;
MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG GQLGGELILT
YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ IREKLKIIVA GGDGTAGWLL
GVVCDLKLSH PPPIATVPLG TGNNLPFAFG WGKKNPGTDR TAVESFLEQV LKAKVMKIDN
WHILMRMKTP KEGGSCDPVA PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM
DAQISYAFHS ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA
TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF VDDGLIEVVG
FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM RMDGEPWKQP LPLDDETVMV
EISHLGQVNM LATHDCRSRS VFDPSTPRHQ DGAEDYDDNE DDSVAEGEEF RKFGAADTFK
IPDEGEHSNK KGRASRRRNS NVHGWSHVL
