DGOA_RHIME
ID DGOA_RHIME Reviewed; 212 AA.
AC Q92RN8;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Probable 2-dehydro-3-deoxy-6-phosphogalactonate aldolase;
DE EC=4.1.2.21;
DE AltName: Full=2-oxo-3-deoxygalactonate 6-phosphate aldolase;
DE AltName: Full=6-phospho-2-dehydro-3-deoxygalactonate aldolase;
DE AltName: Full=6-phospho-2-keto-3-deoxygalactonate aldolase;
DE Short=KDPGal;
GN Name=dgoA; OrderedLocusNames=R00824; ORFNames=SMc00882;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=1021;
RX PubMed=22797764; DOI=10.1128/jb.00982-12;
RA Geddes B.A., Oresnik I.J.;
RT "Inability to catabolize galactose leads to increased ability to compete
RT for nodule occupancy in Sinorhizobium meliloti.";
RL J. Bacteriol. 194:5044-5053(2012).
CC -!- FUNCTION: Involved in the degradation of galactose via the DeLey-
CC Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol
CC cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) to pyruvate
CC and D-glyceraldehyde-3-phosphate (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-dehydro-3-deoxy-6-phospho-D-galactonate = D-glyceraldehyde
CC 3-phosphate + pyruvate; Xref=Rhea:RHEA:24464, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:58298, ChEBI:CHEBI:59776; EC=4.1.2.21;
CC -!- PATHWAY: Carbohydrate acid metabolism; D-galactonate degradation; D-
CC glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 3/3.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene fail to grow on galactose
CC as sole carbon source. {ECO:0000269|PubMed:22797764}.
CC -!- SIMILARITY: Belongs to the KHG/KDPG aldolase family. {ECO:0000305}.
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DR EMBL; AL591688; CAC45396.1; -; Genomic_DNA.
DR RefSeq; NP_384930.1; NC_003047.1.
DR RefSeq; WP_010968849.1; NC_003047.1.
DR AlphaFoldDB; Q92RN8; -.
DR SMR; Q92RN8; -.
DR STRING; 266834.SMc00882; -.
DR EnsemblBacteria; CAC45396; CAC45396; SMc00882.
DR GeneID; 61602290; -.
DR KEGG; sme:SMc00882; -.
DR PATRIC; fig|266834.11.peg.2214; -.
DR eggNOG; COG0800; Bacteria.
DR HOGENOM; CLU_077795_2_1_5; -.
DR OMA; HGPIPEV; -.
DR UniPathway; UPA00081; UER00520.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0008674; F:2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity; IEA:UniProtKB-EC.
DR GO; GO:0034194; P:D-galactonate catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00452; KDPG_aldolase; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR000887; Aldlse_KDPG_KHG.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR031338; KDPG/KHG_AS_2.
DR PANTHER; PTHR30246; PTHR30246; 1.
DR Pfam; PF01081; Aldolase; 1.
DR PROSITE; PS00160; ALDOLASE_KDPG_KHG_2; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..212
FT /note="Probable 2-dehydro-3-deoxy-6-phosphogalactonate
FT aldolase"
FT /id="PRO_0000428930"
FT ACT_SITE 41
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 130
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000250"
FT BINDING 18
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 70
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 130
FT /ligand="substrate"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 160
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 180..181
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 18
FT /note="Orients the nucleophilic substrate"
FT /evidence="ECO:0000250"
FT SITE 158
FT /note="Plays a major role in determining the
FT stereoselectivity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 212 AA; 21588 MW; EC344AEEE5300551 CRC64;
MDRIPLPPME RPLIAILRGL KPEEAEGVVG ALIETGFTAI EIPLNSPDPF RSIETAVKMA
PAGCLIGAGT VLTTAQVERL ADVGGRLMVS PNVEPAVIRL AATKGMVTMP GVFTPTEALA
AAAAGASGLK FFPASVLGPS GITAIRAVLP GDLEIAAVGG VSEVNFADYA AIGIRSFGLG
SSLYKPGMSA GDVRQRAIAT LAAYDAVYGG QQ
