DGOK_RHIME
ID DGOK_RHIME Reviewed; 306 AA.
AC Q92RN7;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Probable 2-dehydro-3-deoxygalactonokinase DgoK1;
DE EC=2.7.1.58;
DE AltName: Full=2-keto-3-deoxy-galactonokinase;
DE AltName: Full=2-oxo-3-deoxygalactonate kinase;
GN Name=dgoK1; OrderedLocusNames=R00825; ORFNames=SMc00881;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=1021;
RX PubMed=22797764; DOI=10.1128/jb.00982-12;
RA Geddes B.A., Oresnik I.J.;
RT "Inability to catabolize galactose leads to increased ability to compete
RT for nodule occupancy in Sinorhizobium meliloti.";
RL J. Bacteriol. 194:5044-5053(2012).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
RG New York Structural Genomics Research Consortium (NYSGRC);
RT "Crystal structure of a putative 2-dehydro-3-deoxygalactonokinase protein
RT from Sinorhizobium meliloti.";
RL Submitted (JUL-2011) to the PDB data bank.
CC -!- FUNCTION: Involved in the degradation of galactose via the DeLey-
CC Doudoroff pathway. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-dehydro-3-deoxy-D-galactonate + ATP = 2-dehydro-3-deoxy-6-
CC phospho-D-galactonate + ADP + H(+); Xref=Rhea:RHEA:16525,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57989,
CC ChEBI:CHEBI:58298, ChEBI:CHEBI:456216; EC=2.7.1.58;
CC -!- PATHWAY: Carbohydrate acid metabolism; D-galactonate degradation; D-
CC glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 2/3.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene fail to grow on galactose
CC as sole carbon source. {ECO:0000269|PubMed:22797764}.
CC -!- SIMILARITY: Belongs to the DgoK family. {ECO:0000305}.
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DR EMBL; AL591688; CAC45397.1; -; Genomic_DNA.
DR RefSeq; NP_384931.1; NC_003047.1.
DR RefSeq; WP_010968850.1; NC_003047.1.
DR PDB; 3T69; X-ray; 2.55 A; A/B=1-306.
DR PDBsum; 3T69; -.
DR AlphaFoldDB; Q92RN7; -.
DR SMR; Q92RN7; -.
DR STRING; 266834.SMc00881; -.
DR PRIDE; Q92RN7; -.
DR EnsemblBacteria; CAC45397; CAC45397; SMc00881.
DR GeneID; 61602291; -.
DR KEGG; sme:SMc00881; -.
DR PATRIC; fig|266834.11.peg.2215; -.
DR eggNOG; COG3734; Bacteria.
DR HOGENOM; CLU_058005_2_0_5; -.
DR OMA; HFDTFMT; -.
DR UniPathway; UPA00081; UER00519.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0008671; F:2-dehydro-3-deoxygalactonokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0034194; P:D-galactonate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.420.300; -; 1.
DR Gene3D; 3.30.420.310; -; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR007729; DGOK.
DR InterPro; IPR042257; DGOK_C.
DR InterPro; IPR042258; DGOK_N.
DR Pfam; PF05035; DGOK; 1.
DR SUPFAM; SSF53067; SSF53067; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..306
FT /note="Probable 2-dehydro-3-deoxygalactonokinase DgoK1"
FT /id="PRO_0000428931"
FT STRAND 7..12
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 17..22
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 28..35
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 38..44
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 46..56
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 70..72
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 76..78
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 82..88
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 91..94
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 100..104
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 111..114
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 121..125
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 126..134
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 142..146
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 148..157
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 160..167
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 169..179
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 183..186
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 197..208
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 213..227
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 231..251
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 259..264
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 266..278
FT /evidence="ECO:0007829|PDB:3T69"
FT STRAND 282..287
FT /evidence="ECO:0007829|PDB:3T69"
FT HELIX 288..303
FT /evidence="ECO:0007829|PDB:3T69"
SQ SEQUENCE 306 AA; 31733 MW; ACF24F42B776FA6B CRC64;
MTTAGYYAAV DWGTSSFRLW IIGEDGAVLA ERRSAEGMTT AAKTGFHTIL DGHLAAVSAP
AHLPIIICGM AGARQGWKEA GYIETPAALA EIAGRATAIP DVDRDIRILP GLAQRDRRHP
DVMRGEETQL LGAAAHLGAG SHLVCMPGTH SKWVRLADDR VEGFSTFMTG ELFDTIARHT
ILSHAVAEAD TFAAGSAAFT DAVSRTRENP ALATNLLFSV RAGQLLHGTA AADARAQLSG
TLIGLEIAGA LAGSGSVDGV CLVGSGGLGT LYRTALESQG LNVRAVDADE AVRAGLSAAA
RAIWPL
