ID   DGP12_ARATH             Reviewed;         499 AA.
AC   Q9LK70; F4J2Q2;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Putative protease Do-like 12, mitochondrial;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=DEGP12; OrderedLocusNames=At3g16550; ORFNames=MDC8.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11299370; DOI=10.1104/pp.125.4.1912;
RA   Adam Z., Adamska I., Nakabayashi K., Ostersetzer O., Haussuhl K.,
RA   Manuell A., Zheng B., Vallon O., Rodermel S.R., Shinozaki K., Clarke A.K.;
RT   "Chloroplast and mitochondrial proteases in Arabidopsis. A proposed
RT   nomenclature.";
RL   Plant Physiol. 125:1912-1918(2001).
CC   -!- FUNCTION: Putative serine protease.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR   EMBL; AP000373; BAB01154.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75834.2; -; Genomic_DNA.
DR   RefSeq; NP_566552.2; NM_112527.2.
DR   AlphaFoldDB; Q9LK70; -.
DR   SMR; Q9LK70; -.
DR   STRING; 3702.AT3G16550.1; -.
DR   MEROPS; S01.A03; -.
DR   PaxDb; Q9LK70; -.
DR   PRIDE; Q9LK70; -.
DR   EnsemblPlants; AT3G16550.1; AT3G16550.1; AT3G16550.
DR   GeneID; 820903; -.
DR   Gramene; AT3G16550.1; AT3G16550.1; AT3G16550.
DR   KEGG; ath:AT3G16550; -.
DR   Araport; AT3G16550; -.
DR   eggNOG; KOG1320; Eukaryota.
DR   HOGENOM; CLU_020120_10_2_1; -.
DR   InParanoid; Q9LK70; -.
DR   OMA; THTERCY; -.
DR   OrthoDB; 594175at2759; -.
DR   PhylomeDB; Q9LK70; -.
DR   PRO; PR:Q9LK70; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LK70; differential.
DR   Genevisible; Q9LK70; AT.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR041517; DEGP_PDZ.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF13180; PDZ_2; 1.
DR   Pfam; PF17815; PDZ_3; 2.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Mitochondrion; Protease; Reference proteome; Serine protease;
KW   Transit peptide.
FT   TRANSIT         1..24
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..499
FT                   /note="Putative protease Do-like 12, mitochondrial"
FT                   /id="PRO_0000045836"
FT   DOMAIN          272..356
FT                   /note="PDZ"
FT   REGION          94..259
FT                   /note="Serine protease"
FT   ACT_SITE        110
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        144
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        222
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   499 AA;  56188 MW;  016D4BA02CA69BE1 CRC64;
     MLFRSCVGMV SRYSRALLPT ITISSRIATI VLPFALTRGR KIHTMSKDEE WWKKIRKSPP
     VDELMLESVV EVFTDSTKYS KVKPWQTLNQ ESYGGSGFAI AGKKILTNAH VVEGMNDHIF
     VHVKRHGSQV KYKAKVQKIA HECDLAILEI DSDEFWKGMN PLEFGDIPPL NEIVYVVGYP
     KAGETICVTK GVVTGVKTGN YLRSSTKLLT IHIDATTYGG NSGGPVITGD KVLGVLFQIL
     GDKKSTGVVI PTPIIRHFIT GAEESSHNAV FGSLVLSCQS MKNAQIRNHF KMSPETTGIL
     INKINSSSGA HKILRKDDII LAIDGVPVLS EMRRISFNHF ISMKKPDENI LVKVLRKGKE
     HEYNISLKPV KPHIQVQQYY NLPSYYIFGG FVFVPLTKSY IDDKYYKITD EQHVIISQVM
     PDDINKGYSN FKDLQVEKVN GVKVKNLKHL RELIEGCFSK DLRLDLENDK VMVLNYESAK
     KATFEILERH NIKSAWASE