ADA2B_MOUSE
ID ADA2B_MOUSE Reviewed; 453 AA.
AC P30545; F8VQ23;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 02-JUN-2021, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Alpha-2B adrenergic receptor;
DE AltName: Full=Alpha-2B adrenoreceptor;
DE Short=Alpha-2B adrenoceptor;
DE Short=Alpha-2BAR;
GN Name=Adra2b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DBA/2J; TISSUE=Liver;
RX PubMed=1336396; DOI=10.1016/0167-4781(92)90126-k;
RA Chen W.-M., Chang A.C., Shie B.J., Chang Y.-H., Chang N.-C.A.;
RT "Molecular cloning and characterization of a mouse alpha 2C2 adrenoceptor
RT subtype gene.";
RL Biochim. Biophys. Acta 1171:219-223(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1354956; DOI=10.1016/s0006-291x(05)81544-x;
RA Chruscinski A.J., Link R.E., Daunt D.A., Barsh G.S., Kobilka B.K.;
RT "Cloning and expression of the mouse homolog of the human alpha 2-C2
RT adrenergic receptor.";
RL Biochem. Biophys. Res. Commun. 186:1280-1287(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Alpha-2 adrenergic receptors mediate the catecholamine-
CC induced inhibition of adenylate cyclase through the action of G
CC proteins.
CC -!- SUBUNIT: Interacts with RAB26. Interacts with PPP1R9B. Interacts with
CC GGA1, GGA2 and GGA3. {ECO:0000250|UniProtKB:P18089}.
CC -!- INTERACTION:
CC P30545; Q14232: EIF2B1; Xeno; NbExp=2; IntAct=EBI-491084, EBI-491065;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P18089};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:P18089}.
CC Note=Interaction with RAB26, GGA1, GGA2 and GGA3 mediates transport
CC from the Golgi to the cell membrane. {ECO:0000250|UniProtKB:P18089}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Adrenergic receptor subfamily. ADRA2B sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA37131.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M94583; AAA73895.1; -; Genomic_DNA.
DR EMBL; L00979; AAA37131.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL731836; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S28221; S28221.
DR RefSeq; NP_033763.2; NM_009633.3.
DR AlphaFoldDB; P30545; -.
DR SMR; P30545; -.
DR IntAct; P30545; 1.
DR STRING; 10090.ENSMUSP00000071798; -.
DR BindingDB; P30545; -.
DR ChEMBL; CHEMBL2405; -.
DR DrugCentral; P30545; -.
DR iPTMnet; P30545; -.
DR PhosphoSitePlus; P30545; -.
DR MaxQB; P30545; -.
DR PaxDb; P30545; -.
DR PRIDE; P30545; -.
DR Antibodypedia; 72874; 131 antibodies from 26 providers.
DR DNASU; 11552; -.
DR Ensembl; ENSMUST00000071902; ENSMUSP00000071798; ENSMUSG00000058620.
DR GeneID; 11552; -.
DR KEGG; mmu:11552; -.
DR CTD; 151; -.
DR MGI; MGI:87935; Adra2b.
DR VEuPathDB; HostDB:ENSMUSG00000058620; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000161915; -.
DR InParanoid; P30545; -.
DR OMA; CEPQAVP; -.
DR OrthoDB; 737211at2759; -.
DR PhylomeDB; P30545; -.
DR TreeFam; TF316350; -.
DR Reactome; R-MMU-390696; Adrenoceptors.
DR Reactome; R-MMU-392023; Adrenaline signalling through Alpha-2 adrenergic receptor.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-418597; G alpha (z) signalling events.
DR BioGRID-ORCS; 11552; 5 hits in 74 CRISPR screens.
DR PRO; PR:P30545; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P30545; protein.
DR Bgee; ENSMUSG00000058620; Expressed in placenta labyrinth and 64 other tissues.
DR GO; GO:0009986; C:cell surface; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0004935; F:adrenergic receptor activity; IMP:MGI.
DR GO; GO:0004938; F:alpha2-adrenergic receptor activity; IDA:MGI.
DR GO; GO:0051379; F:epinephrine binding; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0032148; P:activation of protein kinase B activity; ISO:MGI.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0071875; P:adrenergic receptor signaling pathway; ISO:MGI.
DR GO; GO:0001525; P:angiogenesis; IMP:MGI.
DR GO; GO:0007565; P:female pregnancy; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0000165; P:MAPK cascade; IMP:MGI.
DR GO; GO:0030168; P:platelet activation; IEA:InterPro.
DR GO; GO:0045777; P:positive regulation of blood pressure; ISO:MGI.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:MGI.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR GO; GO:0070474; P:positive regulation of uterine smooth muscle contraction; ISO:MGI.
DR GO; GO:0035624; P:receptor transactivation; ISO:MGI.
DR GO; GO:0003056; P:regulation of vascular associated smooth muscle contraction; ISO:MGI.
DR InterPro; IPR002233; ADR_fam.
DR InterPro; IPR000207; ADRA2B_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01103; ADRENERGICR.
DR PRINTS; PR00559; ADRENRGCA2BR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Lipoprotein;
KW Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..453
FT /note="Alpha-2B adrenergic receptor"
FT /id="PRO_0000069096"
FT TOPO_DOM 1..17
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 18..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 43..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 55..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 81..90
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 91..113
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 114..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 136..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 159..174
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 175..198
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 199..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 376..399
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 400..408
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 409..432
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 433..453
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 214..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..271
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 97
FT /note="Implicated in ligand binding"
FT /evidence="ECO:0000250"
FT SITE 181
FT /note="Implicated in catechol agonist binding"
FT /evidence="ECO:0000250"
FT SITE 185
FT /note="Implicated in catechol agonist binding"
FT /evidence="ECO:0000250"
FT LIPID 445
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT DISULFID 90..169
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 202
FT /note="V -> L (in Ref. 1; AAA73895)"
FT /evidence="ECO:0000305"
FT CONFLICT 227
FT /note="H -> RPG (in Ref. 1; AAA73895)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 453 AA; 50443 MW; BB3FFA3D7B1D4B0F CRC64;
MSGPAMVHQE PYSVQATAAI ASAITFLILF TIFGNALVIL AVLTSRSLRA PQNLFLVSLA
AADILVATLI IPFSLANELL GYWYFWRAWC EVYLALDVLF CTSSIVHLCA ISLDRYWAVS
RALEYNSKRT PRRIKCIILT VWLIAAVISL PPLIYKGDQR PEPHGLPQCE LNQEAWYILA
SSIGSFFAPC LIMILVYLRI YVIAKRSHCR GLGAKRGSGE GESKKPHPAA GGVPASAKVP
TLVSPLSSVG EANGHPKPPR EKEEGETPED PEARALPPNW SALPRSVQDQ KKGTSGATAE
KGAEEDEEEV EECEPQTLPA SPASVFNPPL QQPQTSRVLA TLRGQVLLSK NVGVASGQWW
RRRTQLSREK RFTFVLAVVI GVFVVCWFPF FFSYSLGAIC PQHCKVPHGL FQFFFWIGYC
NSSLNPVIYT IFNQDFRRAF RRILCRQWTQ TGW
