DGR2_YEAST
ID DGR2_YEAST Reviewed; 852 AA.
AC P32330; D6VXG9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=2-deoxy-glucose resistant protein 2;
GN Name=DGR2; OrderedLocusNames=YKL121W; ORFNames=YKL525;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1514329; DOI=10.1002/yea.320080410;
RA Colleaux L., Richard G.-F., Thierry A., Dujon B.;
RT "Sequence of a segment of yeast chromosome XI identifies a new
RT mitochondrial carrier, a new member of the G protein family, and a protein
RT with the PAAKK motif of the H1 histones.";
RL Yeast 8:325-336(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=19004802; DOI=10.1073/pnas.0803090105;
RA Ralser M., Wamelink M.M., Struys E.A., Joppich C., Krobitsch S., Jakobs C.,
RA Lehrach H.;
RT "A catabolic block does not sufficiently explain how 2-deoxy-D-glucose
RT inhibits cell growth.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:17807-17811(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- DISRUPTION PHENOTYPE: Increases cellular tolerance to 2-deoxy-glucose.
CC {ECO:0000269|PubMed:19004802}.
CC -!- MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the WD repeat DGR2 family. {ECO:0000305}.
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DR EMBL; S44213; AAB23072.2; -; Genomic_DNA.
DR EMBL; Z28121; CAA81962.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09039.1; -; Genomic_DNA.
DR PIR; S25359; S25359.
DR RefSeq; NP_012801.1; NM_001179687.1.
DR AlphaFoldDB; P32330; -.
DR BioGRID; 34014; 131.
DR IntAct; P32330; 1.
DR STRING; 4932.YKL121W; -.
DR iPTMnet; P32330; -.
DR MaxQB; P32330; -.
DR PaxDb; P32330; -.
DR PRIDE; P32330; -.
DR EnsemblFungi; YKL121W_mRNA; YKL121W; YKL121W.
DR GeneID; 853738; -.
DR KEGG; sce:YKL121W; -.
DR SGD; S000001604; DGR2.
DR VEuPathDB; FungiDB:YKL121W; -.
DR eggNOG; KOG0283; Eukaryota.
DR GeneTree; ENSGT00940000157557; -.
DR HOGENOM; CLU_008245_0_0_1; -.
DR InParanoid; P32330; -.
DR OMA; WKVINSP; -.
DR BioCyc; YEAST:G3O-31904-MON; -.
DR PRO; PR:P32330; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P32330; protein.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR040324; WDR44/Dgr2.
DR PANTHER; PTHR14221; PTHR14221; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..852
FT /note="2-deoxy-glucose resistant protein 2"
FT /id="PRO_0000051479"
FT REPEAT 171..210
FT /note="WD 1"
FT REPEAT 278..316
FT /note="WD 2"
FT REPEAT 318..358
FT /note="WD 3"
FT REPEAT 426..471
FT /note="WD 4"
FT REPEAT 476..515
FT /note="WD 5"
FT REPEAT 651..689
FT /note="WD 6"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 723..748
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 731..746
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 716
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 852 AA; 96022 MW; A673BAF9AF22B608 CRC64;
MFKSKTSTLS YDETPNSNEG DRNATPVNPK EKSQTKHLNI PGDRSRHSSI ADSKRSSSRY
DGGYSADIIP AQLRFIDNID YGTRLRKTLH RNSVVSNGYN KLSENDRWYF DLFDRKYFEN
YLEEPTYIKI FKKKEGLEQF DRMFLAQELK IPDVYKSTTY QGEPAVANSE LFKNSICCCT
FSHDGKYMVI GCKDGSLHLW KVINSPVKRS EMGRSEKSVS ASRANSLKIQ RHLASISSHN
GSISSNDLKP SDQFEGPSKQ LHLYAPVFYS DVFRVFMEHA LDILDANWSK NGFLITASMD
KTAKLWHPER KYSLKTFVHP DFVTSAIFFP NDDRFIITGC LDHRCRLWSI LDNEVSYAFD
CKDLITSLTL SPPGGEYTII GTFNGYIYVL LTHGLKFVSS FHVSDKSTQG TTKNSFHPSS
EYGKVQHGPR ITGLQCFFSK VDKNLRLIVT TNDSKIQIFD LNEKKPLELF KGFQSGSSRH
RGQFLMMKNE PVVFTGSDDH WFYTWKMQSF NLSAEMNCTA PHRKKRLSGS MSLKGLLRIV
SNKSTNDECL TETSNQSSSH TFTNSSKNVL QTQTVGSQAI KNNHYISFHA HNSPVTCASI
APDVAIKNLS LSNDLIFELT SQYFKEMGQN YSESKETCDN KPNHPVTETG GFSSNLSNVV
NNVGTILITT DSQGLIRVFR TDILPEIRKK IIEKFHEYNL FHLEAAGKIN NHNNDSILEN
RMDERSSTED NEFSTTPPSN THNSRPSHDF CELHPNNSPV ISGMPSRASA IFKNSIFNKS
NGSFISLKSR SESTSSTVFG PHDIPRVSTT YPKLKCDVCN GSNFECASKN PIAGGDSGFT
CADCGTILNN FR
